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His6-Halo-TEV-Akt2
(Plasmid #87606)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 87606 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCS2+8
  • Backbone size w/o insert (bp) 4095
  • Total vector size (bp) 6595
  • Vector type
    Mammalian Expression ; Zebrafish (Danio rerio) expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    AKT 2
  • Alt name
    Protein Kinase B-beta
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2500
  • GenBank ID
    NM_001626.5
  • Entrez Gene
    AKT2 (a.k.a. HIHGHH, PKBB, PKBBETA, PRKBB, RAC-BETA)
  • Tag / Fusion Protein
    • His-Halo-Tev (N terminal on insert)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Note: There are three additional amino acid residues, WKK, appended to the C-terminus of the Akt2 insert. These residues do not affect protein function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    His6-Halo-TEV-Akt2 was a gift from Yimon Aye (Addgene plasmid # 87606 ; http://n2t.net/addgene:87606 ; RRID:Addgene_87606)
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