-
Plasmid#86666PurposepCAGGS/ES-M21EnvA-VSVg-WPRE, used to package pseudotyped lentivirusDepositorInsertCANE-LV envelope
UseLentiviral, Mouse Targeting, and Synthetic Biolog…TagsExpressionMammalianMutationPromoterCAGAvailable sinceFeb. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
CAG-Cremyc-2A-GFP
Plasmid#116879PurposeExpress Cre enzyme in mammalian cells with floxed genes to achieve somatic knockout. GFP is simultaneously expressed for convenient tracking the transfected cells.DepositorInsertCremyc-2A-GFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCAG-H2B-mRuby3-IRES-eGtACR1-ST
Plasmid#108960PurposeAnion channelrhodopsin GtACR1 fused to ER export signal and targeted to the neuronal soma and proximal dendrites under the control of internal ribosome entry sequenceDepositorInserteGtACR1-ST
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
T/brachyury(2A-Dre)
Plasmid#140761PurposeTargeting vector to T/brachyury gene locus for induction of P2A-fused Dre, with PGK-EM7-puroR drug resistant cassette flanked by FRT(F3) sequence.DepositorInsertDre
UseMouse TargetingTagsExpressionMutationPromoterEndogenous T/brachyuryAvailable sinceJune 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAT-9222
Plasmid#124222PurposeCloning plasmid for EGxFP assay with self-targeting gRNA-sDepositorInsertself-targeting gRNA between EGFP halves
UseCRISPRTagsExpressionMammalianMutationPromoterCAGAvailable sinceNov. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
PRDM9(KRAB-SSXRD-PR/SET-ZF1-dC)-Cas9
Plasmid#184464PurposePlasmid encoding PRDM9-Cas9 fusion under CMV promoterDepositorInsertPRDM9(KRAB-SSXRD-PR/SET-ZF1-dC)-Cas9 (PRDM9 Human)
UseCRISPRTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceApril 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCAG-ChroME2f-mRuby2-ST
Plasmid#170160PurposeCation channelrhodopsin ChroME2f fused to mRuby2 fluorophore and targeted to the neuronal soma and proximal dendritesDepositorInsertChroME2f-mRuby2-ST
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
ROSA26(MultiFPsΔPuro)
Plasmid#140759PurposeTargeting vector to Gt(ROSA)26 locus for conditional expression of distinct FPs (Venus, mCherry, and mCerulean) responding to each site-specific-recombinase activity (Cre, Dre, and phiC31o).DepositorInsertspuromycin-N-acetyltransferase
Venus
mCherry
mCerulean
UseMouse TargetingTagsExpressionMutationPromoterCAGGSAvailable sinceJune 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
PRDM9dC(KRAB-SSXRD-PR/SET-dC)-Cas9
Plasmid#186699PurposePlasmid encoding PRDM9dC-Cas9 fusion under CMV promoterDepositorInsertPRDM9dC(KRAB-SSXRD-PR/SET-dC)-Cas9 (PRDM9 Human)
UseCRISPRTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceJuly 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
Fyn-Fyn Biosensor
Plasmid#124655PurposeFRET biosensor. Fyn-tagged biosensor targeting to membraneDepositorInsertSynthetic gene containing ECFP-SH2 domain-substrate-linker-YPet
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
myc-hRAD18
Plasmid#68827PurposeExpresses c-myc-tagged human RAD18DepositorInsertc-myc tagged human RAD18 (RAD18 Human)
UseTagsc-mycExpressionMammalianMutationPromoterCAGAvailable sinceOct. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCX-2A-2A
Plasmid#74670Purposebased on pCX-C1 with F2A1/F2A2 insertedDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterpCAGGSAvailable sinceFeb. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBW2656_pCAG-PV1-nMag-L1-CTD-ENTRY-BGHpA
Plasmid#108849PurposeInsert protein fragment into KpnI/EcoRI to fuse to nMag tag on N-terminusDepositorTypeEmpty backboneUseSynthetic BiologyTagsnMagExpressionMammalianMutationPromoterAvailable sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBW2655_pCAG-PV1-NTD-ENTRY-L1-pMag-NLS-BGHpA
Plasmid#108848PurposeInsert protein fragment into MluI/BspEI to fuse to pMag/NLS tags on C-terminusDepositorTypeEmpty backboneUseSynthetic BiologyTagspMag,NLSExpressionMammalianMutationPromoterAvailable sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBW2653_pCAG-PV1-NTD-ENTRY-L1-nMag-NLS-BGHpA
Plasmid#108846PurposeInsert protein fragment into MluI/BspEI to fuse to nMag/NLS tags on C-terminusDepositorTypeEmpty backboneUseSynthetic BiologyTagsnMag,NLSExpressionMammalianMutationPromoterAvailable sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBW2067_pCAG-PV1-GAI-L1-CTD-ENTRY-BGHpA
Plasmid#108845PurposeInsert protein fragment into KpnI/EcoRI to fuse to GAI tag on N-terminusDepositorTypeEmpty backboneUseSynthetic BiologyTagsGAIExpressionMammalianMutationPromoterAvailable sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBW2065_pCAG-PV1-GID1-L1-CTD-ENTRY-BGHpA
Plasmid#108843PurposeInsert protein fragment into KpnI/EcoRI to fuse to GID1 tag on N-terminusDepositorTypeEmpty backboneUseSynthetic BiologyTagsGID1ExpressionMammalianMutationPromoterAvailable sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBW1309_pCAG-PV1-FKBP-L1-CTD-ENTRY-BGHpA
Plasmid#108837PurposeInsert protein fragment into KpnI/EcoRI to fuse to FKBP tag on N-terminusDepositorTypeEmpty backboneUseSynthetic BiologyTagsFKBPExpressionMammalianMutationPromoterAvailable sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBW1312_pCAG-PV1-NTD-ENTRY-L1-ABI-NLS-BGHpA
Plasmid#108840PurposeInsert protein fragment into MluI/BspEI to fuse to ABI/NLS tags on C-terminusDepositorTypeEmpty backboneUseSynthetic BiologyTagsABI,NLSExpressionMammalianMutationPromoterAvailable sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBW1313_pCAG-PV1-PYL-L1-CTD-ENTRY-BGHpA
Plasmid#108841PurposeInsert protein fragment into KpnI/EcoRI to fuse to PYL tag on N-terminusDepositorTypeEmpty backboneUseSynthetic BiologyTagsPYLExpressionMammalianMutationPromoterAvailable sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only