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Addgene
Showing: 521 - 540 of 613 results

  1. pBI-MCS-EYFP

    Plasmid
    #58855
    Purpose
    The tet-responsive reporter pBI-MCS-EGFP (Addgene plasmid 16542) was modified to express YFP instead of GFP
    Depositor
    Joshua Leonard
    Article
    Daringer et al ACS Synth Biol. 2014 Mar 11.
    Insert
    enhanced yellow fluorescent protein
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Oct. 27, 2014
    Availability
    Academic Institutions and Nonprofits only
    58855-plasmid-map-sequence-id-98989

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  2. pcDNA3-Lyn-GR-AKAR3

    Plasmid
    #173016
    Purpose
    Plasma membrane-targeted variant of improved green-red FRET-based PKA activity reporter.
    Depositor
    Jin Zhang
    Article
    Mo et al Nat Commun. 2020 Apr 15;11(1):1848. doi:
    Insert
    Lyn-GR-AKAR3
    Use
    Tags
    EGFP, N-terminal targeting sequence from Lyn kina…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Dec. 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    173016-plasmid-map-sequence-id-347656

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  9. pRRLsin-SV40 T antigen-IRES-mCherry

    Plasmid
    #58993
    Purpose
    lentiviral expression of SV40 large T antigen and mCherry red fluorescent reporter
    Depositor
    Snorri Thorgeirsson
    Article
    Holczbauer et al Gastroenterology. 2013 Jul;145(1):221-31
    Inserts
    CMV enhancer-chicken beta actin promoter
    SV40 large T antigen
    IRES-mCherry red fluorescent protein
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    EcoRV site in MCS converted to BamHI site
    Promoter
    CMV enhancer-chicken beta actin
    Available since
    Sept. 29, 2014
    Availability
    Academic Institutions and Nonprofits only
    58993-plasmid-map-sequence-id-457463

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  10. pCVL TLR 3.1 (Sce target) Ef1a iRFP Puro

    Plasmid
    #50417
    Purpose
    Fluorescent reporter for DNA cleavage/repair in mammalian cells expressing iRFP with selectable puromycin marker.
    Depositor
    Andrew Scharenberg
    Inserts
    eGFP with I-Sce I TS
    +3 mCherry
    Use
    Lentiviral
    Tags
    T2A dislinker and iRFP
    Expression
    Mammalian
    Mutation
    embedded I-Sce I TS from 163-185
    Promoter
    SFFV
    Available since
    Jan. 14, 2014
    Availability
    Academic Institutions and Nonprofits only
    50417-plasmid-map-sequence-id-75349

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  13. BTK-APEX2-P2A-mKate2

    Plasmid
    #67653
    Purpose
    Mammalian expression vector driving BTK-APEX2 (Human BTK-PH domain, [marks PI(3,4,5)P3] fused to EM peroxidase marker). Also contains bicistronially expressed cytoplasmic red fluorescent reporter.
    Depositor
    Rob Parton
    Article
    Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:
    Insert
    BTK-PH-APEX2
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Dec. 7, 2015
    Availability
    Academic Institutions and Nonprofits only
    67653-plasmid-map-sequence-id-116993

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  15. pVCS1

    Plasmid
    #231615
    Purpose
    Cumate and vanillate inducible toggle switch reported by mKate and/or sfGFP fluorescence signals; has RBS1 at R1 site and RBS1 at R2 site.
    Depositor
    Hans Christopher Bernstein
    Article
    Chan et al bioRxiv 2024.07.20.604438
    Insert
    Pcym-VanR-mKate2-Pvan-CymR-sfGFP
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Pvan/Pcym
    Available since
    March 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    231615-plasmid-map-sequence-id-464094

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  16. pVCS9

    Plasmid
    #231623
    Purpose
    Cumate and vanillate inducible toggle switch reported by mKate and/or sfGFP fluorescence signals; has RBS3 at R1 site and RBS3 at R2 site.
    Depositor
    Hans Christopher Bernstein
    Article
    Chan et al bioRxiv 2024.07.20.604438
    Insert
    Pcym-VanR-mKate2-Pvan-CymR-sfGFP
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Pvan/Pcym
    Available since
    March 19, 2025
    Availability
    Academic Institutions and Nonprofits only
    231623-plasmid-map-sequence-id-464087

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  17. pVCS8

    Plasmid
    #231622
    Purpose
    Cumate and vanillate inducible toggle switch reported by mKate and/or sfGFP fluorescence signals; has RBS3 at R1 site and RBS2 at R2 site.
    Depositor
    Hans Christopher Bernstein
    Article
    Chan et al bioRxiv 2024.07.20.604438
    Insert
    Pcym-VanR-mKate2-Pvan-CymR-sfGFP
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Pvan/Pcym
    Available since
    March 19, 2025
    Availability
    Academic Institutions and Nonprofits only
    231622-plasmid-map-sequence-id-464071

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  18. pVCS6

    Plasmid
    #231620
    Purpose
    Cumate and vanillate inducible toggle switch reported by mKate and/or sfGFP fluorescence signals; has RBS2 at R1 site and RBS3 at R2 site.
    Depositor
    Hans Christopher Bernstein
    Article
    Chan et al bioRxiv 2024.07.20.604438
    Insert
    Pcym-VanR-mKate2-Pvan-CymR-sfGFP
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Pvan/Pcym
    Available since
    March 19, 2025
    Availability
    Academic Institutions and Nonprofits only
    231620-plasmid-map-sequence-id-464093

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  19. pVCS5

    Plasmid
    #231619
    Purpose
    Cumate and vanillate inducible toggle switch reported by mKate and/or sfGFP fluorescence signals; has RBS2 at R1 site and RBS2 at R2 site.
    Depositor
    Hans Christopher Bernstein
    Article
    Chan et al bioRxiv 2024.07.20.604438
    Insert
    Pcym-VanR-mKate2-Pvan-CymR-sfGFP
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Pvan/Pcym
    Available since
    March 19, 2025
    Availability
    Academic Institutions and Nonprofits only
    231619-plasmid-map-sequence-id-464091

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  20. pVCS4

    Plasmid
    #231618
    Purpose
    Cumate and vanillate inducible toggle switch reported by mKate and/or sfGFP fluorescence signals; has RBS2 at R1 site and RBS1 at R2 site.
    Depositor
    Hans Christopher Bernstein
    Article
    Chan et al bioRxiv 2024.07.20.604438
    Insert
    Pcym-VanR-mKate2-Pvan-CymR-sfGFP
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Pvan/Pcym
    Available since
    March 19, 2025
    Availability
    Academic Institutions and Nonprofits only
    231618-plasmid-map-sequence-id-464053

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Showing: 521 - 540 of 613 results