Addgene

We narrowed to 11,151 results for: cel.2

Showing: 521 - 540 of 11151 results

Results per page:

Sort results by:

psiCHECK-2-Rhoc-wt_3'UTR

Plasmid

#31884

Depositor

Robert Blelloch

Article

Subramanyam et al Nat Biotechnol. 2011 May;29(5):443-8. Ep

Insert

Rhoc wild-type 3'UTR (RHOC Human)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Mutation

Promoter

SV40

Available since

Sept. 14, 2011

Availability

Academic Institutions and Nonprofits only
pCDNA3-SREBP-2 (A1134-1135)

Plasmid

#204201

Purpose

Mammalian expression of SREBP-2

Depositor

Allan Weissman

Article

Yang et al J Biol Chem. 2023 Oct 12;299(12):105346.

Insert

SREBP-2 (SREBF2 Human)

Use

Tags

T7x3

Expression

Mammalian

Mutation

Gly to Ala 1134 to 1135

Promoter

Available since

April 8, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
CMV5.1-SARS-CoV-2 Nsp3

Plasmid

#204205

Purpose

Mammalian expression of SARS-CoV-2 Nsp3

Depositor

Allan Weissman

Article

Yang et al J Biol Chem. 2023 Oct 12;299(12):105346.

Insert

SARS-CoV-2 Nsp3 (Wuhan-Hu-1; https://www.addgene.org/141257/) (ORF1ab )

Use

Tags

FLAGx3

Expression

Mammalian

Mutation

none, from obtained clone

Promoter

Available since

April 8, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-SARS-CoV-2-Nsp3PLpro

Plasmid

#204208

Purpose

Mammalian expression of SARS-CoV-2 Nsp3

Depositor

Allan Weissman

Article

Yang et al J Biol Chem. 2023 Oct 12;299(12):105346.

Insert

SARS-CoV-2 Nsp3 (Wuhan-Hu-1; https://www.addgene.org/141257/) (ORF1ab )

Use

Tags

FLAGx1

Expression

Mammalian

Mutation

PLpro of Nsp3

Promoter

Available since

April 8, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-SARS-CoV-2 Nsp3TM

Plasmid

#204206

Purpose

Mammalian expression of SARS-CoV-2 Nsp3

Depositor

Allan Weissman

Article

Yang et al J Biol Chem. 2023 Oct 12;299(12):105346.

Insert

SARS-CoV-2 Nsp3 (Wuhan-Hu-1; https://www.addgene.org/141257/) (ORF1ab )

Use

Tags

FLAGx1

Expression

Mammalian

Mutation

none, from obtained clone

Promoter

Available since

April 8, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
psiCHECK-2-TGFBR2-wt_3'UTR

Plasmid

#31882

Depositor

Robert Blelloch

Article

Subramanyam et al Nat Biotechnol. 2011 May;29(5):443-8. Ep

Insert

TGFBR2 wild-type 3'UTR (TGFBR2 Human)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Mutation

Promoter

SV40

Available since

Sept. 14, 2011

Availability

Academic Institutions and Nonprofits only
psiCHECK-2-Rhoc-m_3'UTR

Plasmid

#31885

Depositor

Robert Blelloch

Article

Subramanyam et al Nat Biotechnol. 2011 May;29(5):443-8. Ep

Insert

Rhoc mutant 3'UTR (RHOC Human)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Mutation

miR-302b, 372 7-mer seed match mutated from AGCAC…

Promoter

SV40

Available since

Sept. 14, 2011

Availability

Academic Institutions and Nonprofits only
pXW117As(RS-E11-RinA)2

Plasmid

#123146

Purpose

Arsenic sensor with three-layered amplifier cascade. Need to work with plasmid pXWPrinA-gfp2. pSB4A3 carrying J117-30arsR-t-ParsR-30hrpR-30hrpS-t-PhrpLE-31ECF11(ASV)-t-Pecf11-33rinA-t

Depositor

Baojun Wang

Article

Wan et al Nat Chem Biol. 2019 May;15(5):540-548. d

Insert

J117-30arsR-t-ParsR-30hrpR-30hrpS-t-PhrpLE-31ECF11(ASV)-t-Pecf11-33rinA-t

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

J117, ParsR, PhrpLE, Pecf11

Available since

May 9, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pXW109Hg(RS-RinA-E11)2

Plasmid

#123151

Purpose

Mercury sensor with three-layered amplifier cascade. Need to work with plasmid pXWP11-gfp2. pSB4A3 carrying J109-32merR-t-PmerT-30hrpR-30hrpS-t-PhrpLE-30RinA(ASV)-t-PrinA-33ECF11-t

Depositor

Baojun Wang

Article

Wan et al Nat Chem Biol. 2019 May;15(5):540-548. d

Insert

J109-32merR-t-PmerT-30hrpR-30hrpS-t-PhrpLE-30RinA(ASV)-t-PrinA-33ECF11-t

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

J109, PmerT, PhrpLE, PrinA

Available since

May 9, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-SARS-CoV-2 Nsp3TMC111A

Plasmid

#204207

Purpose

Mammalian expression of SARS-CoV-2 Nsp3

Depositor

Allan Weissman

Article

Yang et al J Biol Chem. 2023 Oct 12;299(12):105346.

Insert

SARS-CoV-2 Nsp3 (Wuhan-Hu-1; https://www.addgene.org/141257/) (ORF1ab )

Use

Tags

FLAGx1

Expression

Mammalian

Mutation

Cys to Ala in active site of PLpro

Promoter

Available since

April 8, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
psiCHECK-2-TGFBR2-m3_3'UTR

Plasmid

#31883

Depositor

Robert Blelloch

Article

Subramanyam et al Nat Biotechnol. 2011 May;29(5):443-8. Ep

Insert

TGFBR2 mutant 3'UTR (TGFBR2 Human)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Mutation

Third miR-302b, 372 7-mer seed match mutated from…

Promoter

SV40

Available since

Sept. 14, 2011

Availability

Academic Institutions and Nonprofits only
GFP-Bcl-2-K17R-K22R

Plasmid

#220294

Purpose

mammalian expression of Bcl-2-K17R-K22R tagged with GFP

Depositor

Xinlai Cheng

Article

Wang et al Cell Reports Physical Science, Volume 5,

Insert

Bcl2 (BCL2 Human)

Use

Tags

Emerald GFP

Expression

Mammalian

Mutation

K17R-K22R

Promoter

CMV

Available since

June 17, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pLEX307-SARS-CoV-2-3CL

Plasmid

#160278

Purpose

Allows for constituitive mammalian expression of the SARS-CoV-2 3CL protease via transfection or lentivirus

Depositor

Alejandro Chavez

Article

Resnick et al J Virol. 2021 Jun 24;95(14):e0237420. do

Insert

3CL Protease

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Oct. 6, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pXL003-ishRNA-beta-catenin-2

Plasmid

#36299

Depositor

Sean Palecek

Article

Lian et al Proc Natl Acad Sci U S A. 2012 May 29.

Insert

shRNA of beta-catenin-2 (CTNNB1 Human)

Use

Lentiviral and RNAi

Tags

IRES-RFP-Puro

Expression

Mammalian

Mutation

Promoter

TetO-H1

Available since

Aug. 7, 2012

Availability

Academic Institutions and Nonprofits only
pGEX SHP-2(N)-SH2

Plasmid

#46498

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

SHP-2(N) (PTPN11 Human)

Use

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 1-104

Promoter

Tac

Available since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX SHP-2(NC)-SH2

Plasmid

#46499

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

SHP-2(NC) (PTPN11 Human)

Use

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 1-222

Promoter

Tac

Available since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX SHP-2(C)-SH2

Plasmid

#46497

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

SHP-2(C) (PTPN11 Human)

Use

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 101-222

Promoter

Tac

Available since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
ABHD2-3'UTR-Del-2

Plasmid

#190096

Purpose

examine the activity of ABHD2 3'UTR

Depositor

Bowen Liu

Article

Liu et al Commun Biol. 2022 Aug 13;5(1):816. doi:

Insert

ABHD2 3' UTR delete-2 (ABHD2 Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

deleted the base pairs 7122-7129 refer to NM_0070…

Promoter

Available since

Nov. 9, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
kiCAP-AAV-BI-hTFR1-2

Plasmid

#218797

Purpose

RepCap for AAV production

Depositor

Ben Deverman

Article

Huang et al Science. 2024 Jun 14;384(6701):1220-1227

Insert

AAV Rep-Cap BI-hTFR1-2

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 16, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
161+80pCalb2/mutCREB(-2)-like

Plasmid

#66751

Purpose

luciferase reporter for Calb2 promoter (-161to +80, mutant)

Depositor

Emanuela Felley-Bosco, Rolf Stahel

Article

Kresoja-Rakic et al Oncotarget. 2016 Feb 1. doi: 10.18632/on

Insert

Calb2 promoter (-161bp +80) (CALB2 Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

GCGC (-2) to TAGA

Promoter

Calb2

Available since

Jan. 19, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge