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Plasmid#68046PurposeUbiquitously expressed mitochondrial Tomm-7 tagged wtih oxCerulean-oxVenus (dFP, or double fluorescent protein) for monitoring mitophagy.DepositorInsertpEft-3::Cerulean-Venus::Tomm-7
UseTagsExpressionWormMutationPromoterAvailable sinceAug. 31, 2015AvailabilityAcademic Institutions and Nonprofits only -
RhoC FLARE.sc mCer, mVenus - CA
Plasmid#65424PurposeRhoC CA biosensor single chain; mCerulean-tagged ROCK-RBD, mVenus-tagged RhoC Q63LDepositorInsertRBD, mCerulean, mVenus, RhoC
UseTagsExpressionBacterial, Insect, and Mamm…MutationT153M in mVenus, Q63L in RhoCPromoterAvailable sinceAug. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
RhoC FLARE.sc mCer, mVenus - DN
Plasmid#65423PurposeRhoC DN biosensor single chain; mCerulean-tagged ROCK-RBD, mVenus-tagged RhoC T19NDepositorInsertRBD, mCerulean, mVenus, RhoC
UseTagsExpressionBacterial, Insect, and Mamm…MutationT153M in mVenus, T19N in RhoCPromoterAvailable sinceAug. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
Venus1-Y705F/S727A-STAT3
Plasmid#123176PurposeExpresses Y705F/S727A STAT3 double mutant fused to the 1-157 fragment of Venus in mammalian cellsDepositorInsertSTAT3 tagged with Venus1 (aa 1-157) (STAT3 Human)
UseTagsVenus1 (aa 1-158) for bimolecular fluorescence co…ExpressionMammalianMutationDouble Y705F and S727A substitutionsPromoterAvailable sinceAug. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 Flag-MBD1-14-mVenusC
Plasmid#191393PurposeMBD domain of MBD1 fused to C-terminal part of split mVenusDepositorInsertpcDNA3 Flag-MBD1-14-mVenusC
UseTags3xFLAGExpressionMammalianMutationPromoterCMVAvailable sinceDec. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHDR_NANOS3-T2A-mVenus-PuroTK
Plasmid#222903PurposeHomology directed repair template for knocking in mVenus reporter to NANOS3.DepositorInsertmVenus
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJuly 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
hMLKL NB(1-140)-2xFv-Venus
Plasmid#106076Purposeexpression in mammalian cellsDepositorInserthuman MLKL NB(1-140)-2xFv-Venus (MLKL Human)
UseRetroviral; Tet-on retrovirusTagsVenusExpressionMammalianMutationamino acids 1-140PromoterDox-inducibleAvailable sinceMarch 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
CycA-Venus-Flag (1305)
Plasmid#39853DepositorInsertCyclin A (CCNA2 Human)
UseTagsFlag and VenusExpressionMammalianMutationPromoterAvailable sinceOct. 31, 2012AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-STIM1-Venus-173-C
Plasmid#87619PurposeFull-length STIM1 fused to C-terminal Venus (YFP) fragment; for BiFC.DepositorInsertSTIM1-Venus173-C (STIM1 Human, Synthetic)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTriEx-NTOM20-mVenus-Zdk3
Plasmid#81012PurposeExpression of Zdk1 anchored to mitochondriaDepositorInsertZdk3
UseTags6x His tag, FLAG, NTOM20, and mVenusExpressionBacterial, Insect, and Mamm…MutationPromoterAvailable sinceSept. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pUC19-link-mVenus-FLAG:Kan
Plasmid#211830PurposePlasmid template that encodes the fluorescent protein mVenus with a FLAG tag next to kanamycin resistance gene. This template was used to copy out mVenus-FLAG-Kan for genetic tagging.DepositorInsertmVenus-FLAG-Kan
UseTagsExpressionBacterialMutationNAPromoterAvailable sinceJan. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV.CAG-FLEX.iAChSnFR-Venus-NULL
Plasmid#137960PurposeExpresses FLEXed iAChSnFR (non-binding yellow version) under CAG promoterDepositorArticleInsertiAChSnFR (non-binding yellow variant)
UseAAV and Cre/LoxTagsExpressionMammalianMutationY140A binding site mutationPromoterCAGAvailable sinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
CIp10 - YemVenus (OPT High)
Plasmid#163122PurposeExpresses in Candida albicansDepositorInsertYemVenus OPT High
UseCandida albicans expressionTagsExpressionMutationPromoterAvailable sinceJan. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
Venus-7aa-ActA-pEGFP-C1
Plasmid#177405PurposeTo express Venus fused to mitochondria outer membrane targeting sequence "ActA". Venus faces the cytosol.DepositorInsertActA tail anchor (actA Listeria monocytogenes)
UseTagsVenusExpressionMammalianMutationPromoterCMVAvailable sinceOct. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTriEx-mVenus-Zdk1-VAV2 DH/PH/C1
Plasmid#81133Purposemammalian expression of Zdk1 tagged VAV2 DH/PH/C1 domainDepositorInsertsUseTagsmVenusExpressionBacterial, Insect, and Mamm…MutationPromoterAvailable sinceAug. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Venus-173-N-ORAI1
Plasmid#87618PurposeN-terminal Venus (YFP) fragment fused to full-length ORAI1; for BiFC.DepositorInsertVenus173-N-ORAI1 (ORAI1 Human, Synthetic)
UseTags6xHis, T7 tag (gene 10 leader), and Xpress(TM) ta…ExpressionMammalianMutationPromoterCMVAvailable sinceAug. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTriEx-mVenus-PA-Rac1-T17N
Plasmid#22017PurposeExpression of photoactivatable Rac1 (dominant-negative mutant)DepositorInsertPA-Rac1-T17N (RAC1 Human, Avena sativa (oat))
UseTags6X His and mVenusExpressionBacterial, Insect, and Mamm…MutationRac1 starts at I4 and contains mutation T17N.PromoterCMV, p10Available sinceSept. 29, 2009AvailabilityAcademic Institutions and Nonprofits only -
pEft-3::oxCerulean-oxVenus::Lgg-1
Plasmid#68040PurposeLgg-1 tagged with oxCerulean-oxVenus (dFP, or double fluorescent protein), expressed ubiquitously, for monitoring autophagyDepositorInsertpEft-3::oxCerulean-oxVenus::Lgg-1
UseTagsExpressionWormMutationPromoterAvailable sinceAug. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
betaTrCP mVenus mutant reporter
Plasmid#192459PurposeFluorescent reporter for beta-TrCP with a non-degradable mutation. Used as a control.DepositorInsertCdC25B
UseLentiviralTagsSV40 NLS and mVenusExpressionMammalianMutationaa198-338 of CDC25B. betaTrCP degron sequence (aa…PromoterEF1alphaAvailable sinceOct. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTriex-mVenus-PI-RhoA(Q63L)
Plasmid#86978PurposePI(DM): Photo-activatable/light sensitiveDepositorInsertsUseTags6xHis and mVenusExpressionBacterial, Insect, and Mamm…MutationQ63LPromoterCMVAvailable sinceFeb. 24, 2017AvailabilityAcademic Institutions and Nonprofits only