Addgene

We narrowed to 37,012 results for: Nov

Showing: 521 - 540 of 37012 results

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pOSIP-KH-RBS2-dCas9

Plasmid

#182740

Purpose

Integrative plasmid at the HK022 site in E. coli carrying dCas9 under the control of a Ptet promoter

Depositor

David Bikard

Article

Rousset et al PLoS Genet. 2018 Nov 7;14(11):e1007749.

Insert

dCas9

Use

CRISPR; Integrative vector

Tags

Expression

Mutation

Promoter

Ptet

Available since

May 16, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-Syn-pAceR

Plasmid

#195521

Purpose

Red fluorescent, positive response-polarity voltage indicator under the control of synapsin promoter

Depositor

Vincent Pieribone

Article

Kannan et al Science. 2022 Nov 4;378(6619):eabm8797.

Insert

pAceR

Use

AAV

Tags

Expression

Mammalian

Mutation

VARNAM 78E, 81D, 92N; SI linker

Promoter

Synapsin

Available since

Jan. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-Syn-VARNAM2

Plasmid

#195520

Purpose

Red fluorescent, negative response-polarity voltage indicator under the control of synapsin promoter

Depositor

Vincent Pieribone

Article

Kannan et al Science. 2022 Nov 4;378(6619):eabm8797.

Insert

VARNAM2

Use

AAV

Tags

Expression

Mammalian

Mutation

VARNAM SI linker

Promoter

Synapsin

Available since

Jan. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3-AMPKsub-YPet-NES

Plasmid

#84635

Purpose

Encodes C-terminal (substrate) fragment of bimolecular AMPK/BRSK activity reporter (bimABKAR); cytosol targeted; use in conjunction with pcDNA3-Cerulean-FHA1-NES

Depositor

Jin Zhang

Article

Depry et al Chem Biol. 2015 Nov 19;22(11):1470-9. do

Insert

AMPKsub-YPet-NES

Use

Tags

6xHis, Nuclear export signal (NES), T7 tag (gene …

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 27, 2018

Availability

Academic Institutions and Nonprofits only

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pSJ1679 (pRS315-NOP1pr-GFP11-PUS1)

Plasmid

#86414

Purpose

nuclear split GFP11 reporter without mCherry

Depositor

Sue Jaspersen

Article

Smoyer et al J Cell Biol. 2016 Nov 21;215(4):575-590.

Insert

NOP1pr-GFP11-PUS1

Use

Tags

GFP11 and mCherry

Expression

Yeast

Mutation

Promoter

NOP1pr

Available since

Feb. 14, 2017

Availability

Academic Institutions and Nonprofits only

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p2T-CAG-MCS-P2A-GFP-PuroR

Plasmid

#107186

Purpose

Base plasmid for cloning gene duplication alleles with eGFP reporter.

Depositor

Richard Sherwood

Article

Shen et al Nature. 2018 Nov;563(7733):646-651. doi:

Type

Empty backbone

Use

Tags

Expression

Mutation

Promoter

Available since

Dec. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-CaMKII-VARNAM2

Plasmid

#195527

Purpose

Red fluorescent, negative response-polarity voltage indicator under the control of CaMKII promoter

Depositor

Vincent Pieribone

Article

Kannan et al Science. 2022 Nov 4;378(6619):eabm8797.

Insert

VARNAM2

Use

AAV

Tags

Expression

Mammalian

Mutation

VARNAM SI linker

Promoter

CaMKII

Available since

Jan. 31, 2023

Availability

Academic Institutions and Nonprofits only

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pCF287_EFS-TiCas9-P2A-Puro

Plasmid

#211646

Purpose

EFS-TiCas9-P2A-Puro. Tamoxifen-inducible SpyCas9.

Depositor

Christof Fellmann

Article

Tan et al Cell Rep. 2023 Nov 28;42(11):113339. doi

Insert

Tamoxifen-inducible SpyCas9

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 1, 2024

Availability

Academic Institutions and Nonprofits only

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p3E-2xFYVE_hrs

Plasmid

#67676

Purpose

Multisite gateway entry clone for adding C-terminal fusions of 2xFYVE domain from hrs

Depositor

Rob Parton

Article

Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

Insert

2xFYVEhrs

Use

Gateway multisite 3' entry clone

Tags

Expression

Mutation

Promoter

Available since

Dec. 2, 2015

Availability

Academic Institutions and Nonprofits only

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pAAV_SynP_DIO_tAKARα _WPRE

Plasmid

#196675

Purpose

AAV plasmid for mammalian Cre-dependent expression of tAKARα (genetically encoded sensor for protein kinase A).

Depositor

Haining Zhong

Article

Ma et al Nature. 2022 Nov;611(7937):762-768. doi:

Insert

tAKARα

Use

AAV and Cre/Lox

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 21, 2023

Availability

Academic Institutions and Nonprofits only

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human MTAP

Plasmid

#64077

Purpose

Expression of human 5'-methylthioadenosine phosphorylase

Depositor

Vern Schramm

Article

Guan et al Biochemistry. 2011 Nov 29;50(47):10408-1

Insert

5'-methylthioadenosine phosphorylase (MTAP Synthetic, Human)

Use

Tags

Histidine tag and TEV

Expression

Bacterial

Mutation

Promoter

Available since

July 27, 2015

Availability

Academic Institutions and Nonprofits only
pCMV-dMASS_1

Plasmid

#209764

Purpose

Expression of dMASS_1 in mammalian cells under CMV promoter

Depositor

Andre Berndt

Article

Rappleye et al ACS Sens. 2023 Nov 24;8(11):4233-4244. d

Insert

dMASS_1

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG--uMASS_LF

Plasmid

#209763

Purpose

Expression of uMASS_LF (loss-of-function) in mammalian cells under CAG promoter

Depositor

Andre Berndt

Article

Rappleye et al ACS Sens. 2023 Nov 24;8(11):4233-4244. d

Insert

uMASS_LF

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG-uMASS_1

Plasmid

#209762

Purpose

Expression of uMASS_1 in mammalian cells under CAG promoter

Depositor

Andre Berndt

Article

Rappleye et al ACS Sens. 2023 Nov 24;8(11):4233-4244. d

Insert

uMASS_1

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 13, 2023

Availability

Academic Institutions and Nonprofits only

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hisSUMO-CTCF(F1-F9)

Plasmid

#102859

Purpose

Express DNA binding domain of mouse CTCF in E. coli

Depositor

Gary Stormo

Article

Zuo et al Sci Adv. 2017 Nov 17;3(11):eaao1799. doi

Insert

CTCF (Ctcf Mouse)

Use

Tags

hisSUMO

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 22, 2017

Availability

Academic Institutions and Nonprofits only

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MTK2_005

Plasmid

#123700

Purpose

Encodes pCAG as a Type 2 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

pCAG

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pME-APEX2-NS

Plasmid

#67669

Purpose

Gateway entry clone containing APEX2 with no stop codon

Depositor

Rob Parton

Article

Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

Insert

APEX2

Use

Gateway multisite middle entry clone

Tags

Expression

Mutation

Promoter

Available since

Nov. 30, 2015

Availability

Academic Institutions and Nonprofits only

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pET22b-T5-FB-E25TAG-F6C

Plasmid

#176546

Purpose

Expresses IgG affinity protein FB(F6C) with a TAG stop codon at E25 position

Depositor

Han Xiao

Article

Yu et al Bioconjug Chem. 2018 Nov 21;29(11):3522-

Insert

IgG affinity protein

Use

Tags

His tag

Expression

Bacterial

Mutation

change phenylalanine 6 to cysteine; change glutam…

Promoter

T5

Available since

Dec. 8, 2021

Availability

Academic Institutions and Nonprofits only

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MTK3a_007

Plasmid

#123760

Purpose

Encodes Peroxisome Targeting Sequence as a Type 3a part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

PTS1

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pME-AKT-PH

Plasmid

#67674

Purpose

Gateway entry clone containing AKT-PH with no stop codon

Depositor

Rob Parton

Article

Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

Insert

AKT-PH

Use

Gateway multisite middle entry clone

Tags

Expression

Mutation

Promoter

Available since

Nov. 30, 2015

Availability

Academic Institutions and Nonprofits only

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