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Plasmid#149141PurposeYeast Expression plasmid for SARS-CoV-2 ORF7bDepositorInsertSARS-CoV-2 ORF7b (ORF7b Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable thrombin;StrepIIExpressionYeastMutationwtPromoterpGAL1Available sinceMay 14, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
BB1_L_12_syn_BsaI
Plasmid#89914PurposeLinker to construct BB1 from PCR product with FS1 and FS2 (in with BsaI, out with BbsI)DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAT775
Plasmid#68914PurposeNanoLuc expressed constiutively from PAM1 and rpiL* RBS, pNBU2 backbone, AmpRDepositorInsertNanoLuc
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
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YIp TRP1-MET17
Plasmid#122914PurposeTRP1-MET17 integrating (YIp) vector for stable genomic integration in the BY4741 background.DepositorTypeEmpty backboneUseYeast recombination cloningTagsExpressionYeastMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pColE1_sgRNA_NT_AmpR
Plasmid#119144PurposeEncodes non-targeting sgRNADepositorInsertnon-targeting sgRNA
UseCrisprTagsExpressionMutationPromoterAvailable sinceDec. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pColE1_sgRNA_pos6_AmpR
Plasmid#119149PurposeEncodes sgRNA targeting position 6 in pColE1_70a_deGFP_KanRDepositorInsertsgRNA targeting position 6 in pColE1_70a_deGFP_KanR
UseCrisprTagsExpressionMutationPromoterAvailable sinceNov. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
U6.3>Control.gRNA.f+e
Plasmid#99140PurposeControl gRNA under the regulation of an optimized chicken specific U6 promoterDepositorInsertControl gRNA
UseCRISPRTagsExpressionMutationPromoterChicken U6.3Available sinceAug. 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBS-SKII-3XHA-fSNAP-Hygromycin
Plasmid#188914PurposeIntegration cassette to create C-terminal fusions for fluorescent taggingDepositorInsert3XHA-fSNAP-Hygromycin
UseTagsExpressionYeastMutationPromoterAvailable sinceSept. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDB007(+)
Plasmid#99147PurposeAccessory plasmid for PACE of MjTyrRS variantsDepositorInsertPT7 [SD8] gIII, luxAB; PproK tyrT(Opt, CUA)
UseTagsExpressionBacterialMutationPromoterPT7 and PproKAvailable sinceNov. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pmNeonGreenHO-3xFLAG-G
Plasmid#127914Purposea mammalian expression backbone vector for humanized mNeonGreen-3xFlagDepositorInserthumanized mNeonGreen
UseTagsmNeonGreen-3xFlagExpressionMammalianMutationPromoterAvailable sinceAug. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJJH1295
Plasmid#36914DepositorInsert3x mCherry
UseTagsExpressionBacterial, Mammalian, and Y…MutationPromoterAvailable sinceAug. 15, 2012AvailabilityAcademic Institutions and Nonprofits only -
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pColE1_sgRNA_pos3_AmpR
Plasmid#119146PurposeEncodes sgRNA targeting position 3 in pColE1_70a_deGFP_KanRDepositorInsertsgRNA targeting position 3 in pColE1_70a_deGFP_KanR
UseCrisprTagsExpressionMutationPromoterAvailable sinceDec. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pColE1_sgRNA_pos5_AmpR
Plasmid#119148PurposeEncodes sgRNA targeting position 5 in pColE1_70a_deGFP_KanRDepositorInsertsgRNA targeting position 5 in pColE1_70a_deGFP_KanR
UseCrisprTagsExpressionMutationPromoterAvailable sinceNov. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pColE1_sgRNA_pos2_AmpR
Plasmid#119145PurposeEncodes sgRNA targeting position 2 in pColE1_70a_deGFP_KanRDepositorInsertsgRNA targeting position 2 in pColE1_70a_deGFP_KanR
UseCrisprTagsExpressionMutationPromoterAvailable sinceNov. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4046426
Plasmid#149140PurposeYeast Expression plasmid for SARS-CoV-2 ORF7bDepositorInsertSARS-CoV-2 ORF7b (ORF7b Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable thrombin;3xFLAGExpressionYeastMutationSaccharomyces cerevisiae recode 1PromoterpTEF1Available sinceMay 14, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
BASIC_SEVA_16.10
Plasmid#178914PurposeBASIC DNA assembly vectorDepositorInsertmScarlet counter-selection cassette
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 7, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
pBEL1810
Plasmid#139914PurposeExpression plasmid for LetB mutant L358NDepositorInsertLetB
UseTagsExpressionBacterialMutationLetB(43-877; L358N)-6xHisPromoterAvailable sinceAug. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4046555
Plasmid#149146PurposeYeast Expression plasmid for SARS-CoV-2 nsp3DepositorInsertSARS-CoV-2 nsp3 (ORF1ab Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable thrombin;StrepIIExpressionYeastMutationSaccharomyces cerevisiae recode 1PromoterpTEF1Available sinceMay 14, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits