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Bacterial Strain#182778PurposeRecoded E. coli MG1655 strain with UAG termination function removed (RF1 is deleted); T7RNAP cassette genomically inserted; ompT, rne, and lon inactivatedDepositorBacterial ResistanceSpectinomycinAvailable sinceApril 18, 2023AvailabilityAcademic Institutions and Nonprofits only
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pDY1052 PASTEv3 pCMV-SpCas9-XTEN3-RT(L139P)-(GGS)6-BxbINT
Plasmid#179105PurposepCMV-SpCas9-XTEN3-RT(L139P)-(GGS)6-BxbINTDepositorInsertpCMV-SpCas9-XTEN3-RT(L139P)-(GGS)6-BxbINT
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1α-F-FLEX-mNaChBac-T2A-tdTomato (AAV8)
Viral Prep#60658-AAV8PurposeReady-to-use AAV8 particles produced from pAAV-EF1α-F-FLEX-mNaChBac-T2A-tdTomato (#60658). In addition to the viral particles, you will also receive purified pAAV-EF1α-F-FLEX-mNaChBac-T2A-tdTomato plasmid DNA. CAG-driven, Flp-dependent expression of the voltage-gated Na+ channel mNaChBac-T2A-tdTomato. These AAV preparations are suitable purity for injection into animals.DepositorPromoterEF1αTagsNoneAvailable sinceOct. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pB-CAGGS-dCas9-KRAB-MeCP2
Plasmid#110824PurposePiggyBac compatible plasmid expressing dCas9-KRAB-MeCP2DepositorInsertdCas9-KRAB-MeCP2
UseTagsExpressionMammalianMutationCas9 mutations to make dCas9=D10A+D839A+H840A+N86…PromoterCAGGSAvailable sinceJuly 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDx_mScarlet3
Plasmid#189754PurposeDual expression vector for bacteria and mammalian cells producing mScarlet3 red fluorescent proteinDepositorInsertmScarlet3
UseTags6xHisExpressionBacterial and MammalianMutationPromoterCMV & RhaAvailable sinceMarch 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pICH41331::pU6cm-pegR::EURb7
Plasmid#227695PurposeGolden Gate level 0 acceptor vector for cloning altered epegRNA: altered epegRNA being driven by U6 composite promoter (pU6cm) and terminated by a dual terminator (EURb7).DepositorInsertAltered epegRNA backbone with cloning sites for gRNA and RTT.
UseCRISPRTagsExpressionPlantMutationPromoterU6 compositeAvailable sinceNov. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pENN.AAV.CamKII.HI.GFP-Cre.WPRE.SV40 (AAV9)
Viral Prep#105551-AAV9PurposeReady-to-use AAV9 particles produced from pENN.AAV.CamKII.HI.GFP-Cre.WPRE.SV40 (#105551). In addition to the viral particles, you will also receive purified pENN.AAV.CamKII.HI.GFP-Cre.WPRE.SV40 plasmid DNA. Expression of GFP-Cre from CamKII promoter. These AAV preparations are suitable purity for injection into animals.DepositorPromoterCaMKIITagsGFPAvailable sinceAug. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
SRE reporter vector_559
Plasmid#82686PurposeCloning vector containing serum response element (SRE) followed by an AscI restriction siteDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
VAMP2-pHmScarlet
Plasmid#166890PurposeFor vesicle exocytosis visualization using pHmScarletDepositorInsertVAMP2 (Vamp2 Mouse)
UseTagspHmScarletExpressionMammalianMutationPromoterCMVAvailable sinceApril 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
HDAC1 Flag
Plasmid#13820PurposeMammalian expression of human histone deacetylase 1 with flag tagDepositorInsertHDAC1 (HDAC1 Human)
UseTagsflagExpressionMammalianMutationPromoterAvailable sinceFeb. 19, 2007AvailabilityAcademic Institutions and Nonprofits only -
pENN.AAV.hSyn.Cre.WPRE.hGH (AAV9)
Viral Prep#105553-AAV9PurposeReady-to-use AAV9 particles produced from pENN.AAV.hSyn.Cre.WPRE.hGH (#105553). In addition to the viral particles, you will also receive purified pENN.AAV.hSyn.Cre.WPRE.hGH plasmid DNA. hSyn-driven Cre expression. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagsNoneAvailable sinceJuly 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-fDIO-hM3D(Gq)-mCherry-WPREpA (AAV8)
Viral Prep#154868-AAV8PurposeReady-to-use AAV8 particles produced from pAAV-hSyn-fDIO-hM3D(Gq)-mCherry-WPREpA (#154868). In addition to the viral particles, you will also receive purified pAAV-hSyn-fDIO-hM3D(Gq)-mCherry-WPREpA plasmid DNA. Syn-driven, Flp-dependent hM3D(Gq) receptor with an mCherry reporter for CNO-induced neuronal activation. These AAV preparations are suitable purity for injection into animals.DepositorPromoterHuman Synapsin-1TagsmCherry (Flp-dependent)Available sinceAug. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
EGFP-alphasynuclein-A53T
Plasmid#40823DepositorInsertSNCA (SNCA Human)
UseTagsEGFPExpressionMammalianMutationA53T (aggregates more rapidly than the wild-type …PromoterCMVAvailable sinceMay 8, 2013AvailabilityAcademic Institutions and Nonprofits only -
pMXs-puro GFP-p62
Plasmid#38277DepositorInsertSequestosome-1 (Sqstm1 Mouse)
UseRetroviralTagsEGFPExpressionMammalianMutationPromoterAvailable sinceAug. 17, 2012AvailabilityAcademic Institutions and Nonprofits only -
pDae079 (eMutaT7[transition])
Plasmid#187622PurposeExpresses eMutaT7[TadA-8e], eMutaT7[PmCDA1] and UGI for mutagenizing genes downstream from a T7 promoter in e.coliDepositorInsertsTadA-8e-T7RNAP
PmCDA1-T7RNAP
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-Cre-P2A-dTomato (AAV Retrograde)
Viral Prep#107738-AAVrgPurposeReady-to-use AAV Retrograde particles produced from pAAV-hSyn-Cre-P2A-dTomato (#107738). In addition to the viral particles, you will also receive purified pAAV-hSyn-Cre-P2A-dTomato plasmid DNA. hSyn-driven expression of Cre and dTomato (physically separate). These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagsdTomato (physically separate, not a fusion protein)Available sinceJune 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCAG-luciferase
Plasmid#55764Purposeluciferase reporter for in vivo bioluminescence imagingDepositorInsertCMV enhancer/chicken beta actin promoter
UseTagsExpressionMammalianMutationPromoterCMV enhancer/chicken beta actin promoterAvailable sinceSept. 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-DIO-ChR2(H134R)-eYFP (AAV PHP.eB)
Viral Prep#127090-PHPeBPurposeReady-to-use AAV PHP.eB particles produced from pAAV-CAG-DIO-ChR2(H134R)-eYFP (#127090). In addition to the viral particles, you will also receive purified pAAV-CAG-DIO-ChR2(H134R)-eYFP plasmid DNA. CAG-driven, Cre-dependent expression of humanized channelrhodopsin H134R mutant fused to EYFP, for optogenetic activation. These AAV were produced with the PHPeB serotype, which permits efficient transduction of the central nervous system. These AAV preparations are suitable purity for injection into animals.DepositorPromoterCAGTagsEYFP (Cre-dependent)Available sinceJuly 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pT3-EF1A-MYC-IRES-lucOS
Plasmid#129776PurposeTransposon based vector that expresses human MYC followed by IRES and firefly luciferase and the antigens SIYRYYGL (SIY), SIINFEKL (SIN, OVA257-264), and OVA323-339DepositorInserthuman MYC (MYC Human)
UseTagsOS: SIYRYYGL (SIY), SIINFEKL (SIN, OVA257-264), a…ExpressionMammalianMutationPromoterEF1AAvailable sinceFeb. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
tFucci(CA)5
Plasmid#153521PurposeNew color variant of Fucci (Fluorescent ubiquitination-based cell cycle indicator) : Fucci(CA)5.DepositorInserttFucci(CA)5.
UseTagsAzaleaB5-hCdt1(1/100)Cy(-)_P2A_h2-3-hGem(1/110)ExpressionBacterial and MammalianMutationPromoterCAGAvailable sinceAug. 20, 2020AvailabilityAcademic Institutions and Nonprofits only