Addgene

We narrowed to 34,897 results for: kan

Showing: 5341 - 5360 of 34897 results

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Chr1q_Cassette-Integration_gRNA

Plasmid

#195126

Purpose

gRNA in a third generation Cas9 vector with GFP, targeting location downstream of Chr1q_centromere-targeting_gRNA for positive-negative selection cassette integration

Depositor

Jason Sheltzer

Article

Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

Insert

Chr1q gRNA

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1_Cre

Plasmid

#201198

Purpose

AAV vector for cre expression under EF1 promoter

Depositor

Tetsuo Yamamori

Article

Watakabe et al Neuron. 2023 May 9:S0896-6273(23)00338-0

Inserts

cre
EF1alpha promoter

Use

AAV

Tags

Expression

Bacterial and Mammalian

Mutation

nuclear targeting cre

Promoter

N/A and hEF1alpha promoter

Available since

July 14, 2023

Availability

Academic Institutions and Nonprofits only

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pCDNA5/tom20-pbira(79-c)-ha

Plasmid

#154456

Purpose

expresses tom20-pbira(79-c)-ha/biochemical assay

Depositor

Hyun-Woo Rhee

Article

Kwak et al Proc Natl Acad Sci U S A. 2020 May 15. p

Insert

tom20-pbira(79-c)-ha (TOMM20 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV-F

Available since

Aug. 24, 2020

Availability

Academic Institutions and Nonprofits only

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Dom neg CstF64

Plasmid

#127250

Purpose

Expresses the Dominant Negative CstF64 in mammalian cells; DN blocks polyadenylation

Depositor

Christine Milcarek

Article

Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

Insert

CstF64 delta 282 (CSTF2 Human)

Use

Other

Tags

Expression

Mammalian

Mutation

insert @SanD1:GTCCAGGCGCCTACCCATACGACGTCCCAGACTAC…

Promoter

pEF1a

Available since

July 15, 2019

Availability

Academic Institutions and Nonprofits only

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PLX_307 FDX1

Plasmid

#184495

Purpose

Lentivirus expression for FDX1

Depositor

Peter Tsvetkov, Todd Golub

Article

Tsvetkov et al Science. 2022 Mar 18;375(6586):1254-1261

Insert

FDX1 (FDX1 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

June 28, 2022

Availability

Academic Institutions and Nonprofits only

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pHR-HOXD13-IDR-mCherry-CRY2

Plasmid

#145258

Purpose

Mammalian Expression of Opto HOXD13 IDR

Depositor

Denes Hnisz

Article

Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

Insert

HOXD13wt-IDR (HOXD13 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 22, 2020

Availability

Academic Institutions and Nonprofits only

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pGem-nHyPer:cAPX

Plasmid

#84736

Purpose

Simultaneous expresion of stromal HyPer2 and cytosolic Ascorbate peroxidase (APX) in plant cells

Depositor

Philip Mullineaux

Article

Exposito-Rodriguez et al Nat Commun. 2017 Jun 29;8(1):49. doi: 10

Inserts

APX1 (APX1 Mustard Weed)
HyPer2 targeted to nucleus

Use

Tags

NLS-SV40

Expression

Plant

Mutation

Promoter

pFMV/p35S

Available since

June 30, 2017

Availability

Academic Institutions and Nonprofits only

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pTEF:ATP

Plasmid

#92179

Purpose

HO-TEF1p-ATeam1.03-KanMX4-HO. Codon optimized for yeast version of the ATeam1.03 ATP FRET sensor (originally from Imamura et al. PNAS, 2009 ), expressed via the TEF1 promoter. HO locus integration.

Depositor

Matthias Heinemann

Article

Papagiannakis et al Mol Cell. 2017 Jan 19;65(2):285-295. doi

Insert

ATeam1.03 codon optimized for yeast (Saccharomyces cerevisiae)

Use

Integration and expression into yeast.

Tags

Yeast codon optimized mseCFP (donor) and cp173-mV…

Expression

Yeast

Mutation

Promoter

TEF1p

Available since

June 26, 2017

Availability

Academic Institutions and Nonprofits only

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pCIG AKT3 E17K

Plasmid

#73050

Purpose

Human AKT3 E17K

Depositor

Joseph Gleeson

Article

Baek et al Nat Med. 2015 Dec;21(12):1445-54. doi: 1

Insert

AKT3 E17K (AKT3 Human)

Use

Tags

Expression

Mammalian

Mutation

E17K

Promoter

Available since

April 11, 2016

Availability

Academic Institutions and Nonprofits only

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pAAVTRE3_Clover

Plasmid

#135179

Purpose

AAV expression of TRE(TRE3G)-driven, Clover expression for fluorescent labling

Depositor

Tetsuo Yamamori

Article

Watakabe et al Neuron. 2023 May 9:S0896-6273(23)00338-0

Inserts

Clover
TRE3G

Use

AAV

Tags

Expression

Mammalian

Mutation

a variant of GFP

Promoter

TRE3G

Available since

Jan. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3/hArf5(WT)-mCherry

Plasmid

#79421

Purpose

Expresses C-terminally mCherry-tagged Arf5(WT) in mammalian cells

Depositor

Kazuhisa Nakayama

Article

Makyio et al EMBO J. 2012 May 30;31(11):2590-603. doi

Insert

Arf5 (ARF5 Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

Available since

April 11, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3/hArf3(WT)-mCherry

Plasmid

#79420

Purpose

Expresses C-terminally mCherry-tagged Arf3(WT) in mammalian cells

Depositor

Kazuhisa Nakayama

Article

Makyio et al EMBO J. 2012 May 30;31(11):2590-603. doi

Insert

Arf3 (ARF3 Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

Available since

April 11, 2017

Availability

Academic Institutions and Nonprofits only

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SHMT2-RES

Plasmid

#72880

Purpose

overexpression

Depositor

David Sabatini

Article

Kim et al Nature. 2015 Apr 16;520(7547):363-7. doi

Insert

SHMT2 (SHMT2 Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

silent mutations in site corresponding to shSHMT2…

Promoter

Available since

Feb. 2, 2016

Availability

Academic Institutions and Nonprofits only
P-Ef1a-RPL22-3XHA-P2A-EGFP-T2A-Neo

Plasmid

#170319

Purpose

Expresses HA and EGFP tagged RPL22, neomycin resistance

Depositor

Hemali Phatnani

Article

Gregory et al Cells. 2020 Jun 5;9(6). pii: cells906140

Insert

RPL22 (RPL22 Human)

Use

Lentiviral

Tags

Expression

Mutation

None

Promoter

Available since

May 17, 2021

Availability

Academic Institutions and Nonprofits only

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pSAM2_mCherry_Nkx2.5

Plasmid

#72689

Purpose

Doxycyline regulated expression of Nkx2.5 in mammalian cells

Depositor

Timothy Kamp

Article

Lalit et al Cell Stem Cell. 2016 Mar 3;18(3):354-67.

Insert

Nkx 2.5 (Nkx2-5 Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV-Doxycycline

Available since

Aug. 28, 2017

Availability

Academic Institutions and Nonprofits only

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pCIG AKT3 K177M

Plasmid

#73051

Purpose

Human AKT3 K177M

Depositor

Joseph Gleeson

Article

Baek et al Nat Med. 2015 Dec;21(12):1445-54. doi: 1

Insert

AKT3 K177M (AKT3 Human)

Use

Tags

Expression

Mammalian

Mutation

K177M

Promoter

Available since

March 11, 2016

Availability

Academic Institutions and Nonprofits only

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pEMS2144

Plasmid

#104357

Purpose

AAV plasmid with smCBA promoter driving expression of emerald GFP (EmGFP) reporter

Depositor

Elizabeth Simpson

Article

de Leeuw et al Mol Brain. 2016 May 10;9(1):52. doi: 10.

Insert

ssAAV-smCBA-EmGFP

Use

AAV

Tags

Expression

Mutation

Promoter

smCBA

Available since

July 10, 2019

Availability

Academic Institutions and Nonprofits only

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pCIG AKT3 S472A

Plasmid

#73052

Purpose

Human AKT3 S472A

Depositor

Joseph Gleeson

Article

Baek et al Nat Med. 2015 Dec;21(12):1445-54. doi: 1

Insert

AKT3 S472A (AKT3 Human)

Use

Tags

Expression

Mammalian

Mutation

S472A

Promoter

Available since

March 18, 2016

Availability

Academic Institutions and Nonprofits only

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pLKO_HOXB13_#2

Plasmid

#70094

Purpose

Expression of shRNA to human HOXB13, puromycin selection

Depositor

William Hahn

Article

Pomerantz et al Nat Genet. 2015 Nov;47(11):1346-51. doi:

Insert

HOXB13 (HOXB13 Human)

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
pLKO_HOXB13_#1

Plasmid

#70093

Purpose

Expression of shRNA to human HOXB13, puromycin selection

Depositor

William Hahn

Article

Pomerantz et al Nat Genet. 2015 Nov;47(11):1346-51. doi:

Insert

HOXB13 (HOXB13 Human)

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only