-
Plasmid#154903PurposeExpresses codon optimized catalytically inactive PHGDH in mammalian cellsDepositorInsertPhosphoglycerate dehydrogenase (PHGDH Human)
UseLentiviralTagsExpressionMammalianMutationThree mutations: D175N, R236K, H283APromoterTREAvailable sinceJuly 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pαH-S-GSAS-2P-OMICRON
Plasmid#180593PurposeMammalian cell expression of SARS-CoV-2 Spike protein variant OMICRON with furin side replaced by GSAS and with 2 Proline substitution at 986 and 987DepositorInsertSpike (S-GSAS-2P-OMICRON) (S Severe acute respiratory syndrome coronavirus 2)
UseTagsHRV3C cleavage site, 8X His tag, 2X Strep-Tag IIExpressionMammalianMutationEctodomain (AAs 1-1208); 682-685 (furin site = RR…PromoterAvailable sinceJan. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSH424-ssr
Plasmid#198026PurposeExpresses the gene codying for the Ssr recombinase from Pseudomonas putida DOT-T1EDepositorInsertT0 terminator - Sm resistance cassette
UseTagsExpressionBacterialMutationPromoterSmR promoterAvailable sinceOct. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
-
-
pMT1093 human L1 EN- (ORF2p E43S, D145N) ORF2p-3xFlag (ORFeus-Hs, CMV promoter) in pCEP4 Puro (derivative of pMT646)
Plasmid#213028PurposeExpresses endonuclease dead (EN- ORF2p E43S, D145N) full length human LINE-1 in human cells (codon optimized ORFeus-Hs), with a C-terminal 3xFlag tag on ORF2DepositorInsertHuman LINE-1 (ORFeus-Hs codon optimized sequence, EN- E43S D145N) with ORF2-3xFlag
UseTags3xFlag (ORF2p)ExpressionMammalianMutationE43S D145N double mutant EN- endonuclease catalyt…PromoterCMVAvailable sinceMay 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMT647 human L1 ORF2p-3xFlag only (ORFeus-Hs, CMV promoter, monocistronic construct) in pCEP4 Puro (derivative of pMT646)
Plasmid#213029PurposeExpresses human LINE-1 ORF2 only in human cells (codon optimized ORFeus-Hs), with a C-terminal 3xFlag tag on ORF2DepositorInsertHuman LINE-1 ORF2p only (monocistronic, codon optimized ORFeus-Hs sequence) with ORF2-3xFlag
UseTags3xFlagExpressionMammalianMutationPromoterCMVAvailable sinceMay 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRT006.3 L1 dual-luciferase AI retrotransposition reporter
Plasmid#213031PurposeBi-directional luciferase antisense intron (firefly fluc AI) human LINE-1 retrotransposition reporter (ORFeus-Hs sequence) for sleeping beauty integrationDepositorInsertTet-On Human LINE-1 (ORFeus-Hs) Firefly + Renilla Luciferase Antisense Intron dual retrotransposition marker
UseLuciferaseTagsFirefly luciferase antisense intron (3' UTR)ExpressionMammalianMutationPromoterpTRE bidirectional tet-onAvailable sinceJuly 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEF005-sgRNA-shuffle-in
Plasmid#104441PurposeProvide and shuffle a cassette of AtU6:sgRNA-transRNA into SM-destination vectors (pRW006 and pRW004) with golden gate cloning strategy. Work together with pEF004.DepositorInsertsgRNA-transRNA transcription by U6 promoter
UseCRISPRTagsExpressionBacterial and PlantMutationPromoterAvailable sinceJan. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
Strain: C321.ΔA
Bacterial Strain#68306PurposeGenomically recoded strain of E. coli (no genomic TAG)DepositorBacterial ResistanceBleocin (zeocin)Available sinceSept. 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEF004-sgRNA-shuffle-in
Plasmid#104440PurposeProvide and shuffle a cassette of AtU6:sgRNA-transRNA into SM-destination vectors (pRW006 and pRW004) with golden gate cloning strategy. Work together with pEF005DepositorInsertsgRNA-transRNA transcription by U6 promoter
UseCRISPRTagsExpressionBacterial and PlantMutationPromoterU6 promoterAvailable sinceDec. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
C321.∆A.opt
Bacterial Strain#87359PurposeThis is C321.∆A with additional mutations engineered by Kuznetsov et al. to improve doubling time.DepositorBacterial ResistanceGentamicinSpeciesSyntheticAvailable sinceMay 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
DinoIII-gfp
Plasmid#128141PurposeDinoflagelate backbone for gfp expressionDepositorInsertGreen fluorescence protein
UseTagsExpressionBacterialMutationPromoterDinoflagellate potential promotersAvailable sinceApril 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
DinoIII-arrO
Plasmid#128142PurposeDinoflagellate backbone for rifampin resistant codon optimized geneDepositorInsertRifampin ADP-ribosylating transferase
UseTagsExpressionBacterialMutationPromoterDinolagellate potential promotersAvailable sinceApril 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
DinoIII
Plasmid#128100PurposeDinoflagellate expression vector backbone. XbaI and BglII restriction sites for gene insertion.DepositorInsertRNA complex & upstream/downstream of LHC
UseTagsExpressionBacterialMutationPromoterInserts are potential promotersAvailable sinceApril 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
DinoIII-arrO-N
Plasmid#128143PurposeDinoflagellate backbone with rifampicin resistant gene optimized for Oxyrrhis marinaDepositorInsertRifampin ADP-ribosylating transferase + potential promoter
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCK354
Plasmid#129698PurposeFor insulated expression in Synechocystis 6803. As pCK306 (rhaBAD rhamnose-inducible promoter, YFP, an E. coli-Synechocystis shuttle vector, chromosomal-integration sites) + terminator ECK120010799DepositorInsertsPrhaBAD
yfp
rhaS
Terminator ECK120010799
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEA02
Plasmid#172193PurposeSuicide vector carrying the site attL of the SSR system of pSAM2. This plasmid is integrated into the genome of Actinobacteria by HR when carrying a DNA fragment identical to a region of the genome.DepositorInsertattL
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEA03
Plasmid#172194PurposeSuicide vector carrying the site attR of the SSR system of pSAM2. This plasmid is integrated into the genome of Actinobacteria by HR when carrying a DNA fragment identical to a region of the genome.DepositorInsertattR
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCK351
Plasmid#129696PurposeFor insulated expression in Synechocystis 6803. As pCK306 (rhaBAD rhamnose-inducible promoter, YFP, an E. coli-Synechocystis shuttle vector, chromosomal-integration sites) + terminator ECK120034435DepositorInsertsPrhaBAD
yfp
rhaS
Terminator ECK120034435
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 2, 2019AvailabilityAcademic Institutions and Nonprofits only