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Plasmid#63833PurposeThis plasmid is designed to validate the biophysical model of translational couplingDepositorInsertCFP, mRFP1 and GFPmut3b
UseTagsExpressionBacterialMutationThe intergenic distance between each gene is -4 n…PromoterJ23100Available sinceFeb. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTd-4-15-13-9
Plasmid#63834PurposeThis plasmid is designed to validate the biophysical model of translational couplingDepositorInsertCFP, mRFP1 and GFPmut3b
UseTagsExpressionBacterialMutationThe intergenic distance between each gene is -4 n…PromoterJ23100Available sinceFeb. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTd-4-15-13-11
Plasmid#63836PurposeThis plasmid is designed to validate the biophysical model of translational couplingDepositorInsertCFP, mRFP1 and GFPmut3b
UseTagsExpressionBacterialMutationThe intergenic distance between each gene is -4 n…PromoterJ23100Available sinceFeb. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pXY029 (mEos2-MTSBs)
Plasmid#72652PurposeInducible expression of mEos2-MTSBs in bacteriaDepositorInsertmEos2-MTSBs
UseTagsmEos2ExpressionBacterialMutationPromoterT5-lacAvailable sinceFeb. 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pES004 (pTet-qacR 5)
Plasmid#69033PurposeqacR 5 downstream of a Tet promoterDepositorInsertqacR (qacR Synthetic)
UseTagsHis6 tagExpressionBacterialMutationW61Y, E90Q, F102S, M116Q, Y119L, E120QPromoterpTetAvailable sinceDec. 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pES003 (pTet-qacR 2)
Plasmid#69032PurposeqacR 2 downstream of a Tet promoterDepositorInsertqacR (qacR Synthetic)
UseTagsHis6 tagExpressionBacterialMutationE57Q, E58L, W61Y, E90Q, I99Q, M116Q, L119Y, E120Q…PromoterpTetAvailable sinceSept. 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
pJBL-RNAINS4-C24A
Plasmid#69462PurposeRNA-IN S4 C24A variant 5' UTR sequence controlling expression of superfolder GFP (UTR sequence from Mutalik et al., Nat. Chem. Biol., 2012)DepositorInsertRNA-IN S4 with C24A mutation
UseSynthetic BiologyTagsExpressionBacterialMutationC24A and mutations for S4 variant (from wt)PromoterJ23119 and J23119 (sigma 70)Available sinceSept. 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pJBL-RNAINS4-A25C
Plasmid#69461PurposeRNA-IN S4 A25C variant 5' UTR sequence controlling expression of superfolder GFP (UTR sequence from Mutalik et al., Nat. Chem. Biol., 2012)DepositorInsertRNA-IN S4 with A25C mutation
UseSynthetic BiologyTagsExpressionBacterialMutationA25C and mutations for S4 variant (from wt)PromoterJ23119 and J23119 (sigma 70)Available sinceSept. 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pJBL-RNAINS4-C24AA25C
Plasmid#69463PurposeRNA-IN S4 C24AA25C variant 5' UTR sequence controlling expression of superfolder GFP (UTR sequence from Mutalik et al., Nat. Chem. Biol., 2012)DepositorInsertRNA-IN S4 with C24A, A25C mutations
UseSynthetic BiologyTagsExpressionBacterialMutationC24A, A25C, and mutations for S4 variant (from wt)PromoterJ23119 and J23119 (sigma 70)Available sinceSept. 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCVD403
Plasmid#63740PurposeContains the LT gene probe (HincII fragment) cloned on BamHI linkers into pACYC184DepositorInsertLT gene probe
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBEG R1-ChlorR-R3 #BGV148
Plasmid#48942PurposeContains the Gateway selection cassette (ccdB ChlorR) flanked by AttR1 and AttR3 sites for the creation of Destination vectorsDepositorTypeEmpty backboneUseGateway cloning vectorTagsExpressionMutationPromoterAvailable sinceJune 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAC-EsaR-I70V/D91G/V220A
Plasmid#47647PurposepAC-EsaR with a208g, a272g, c659t mutations in esaRDepositorInsertesaR-I70V/D91G/V220A
UseSynthetic BiologyTagsExpressionBacterialMutationChanged Isoleucine 70 to Valine, Aspartic acid 91…PromoterPlacAvailable sinceSept. 6, 2013AvailabilityAcademic Institutions and Nonprofits only -
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pET21-MRPS11
Plasmid#31343DepositorInsertMRPS11 Mature form (MRPS11 Human)
UseTagsHisExpressionBacterialMutationMature for after removal of the predicted mitocho…PromoterAvailable sinceAug. 24, 2011AvailabilityAcademic Institutions and Nonprofits only -
pET21-MRPL13
Plasmid#31344DepositorInsertmitochondrial ribosomal protein L13 mature form (MRPL13 Human)
UseTagsHisExpressionBacterialMutationMature form after removal of the predicted mitoch…PromoterAvailable sinceAug. 24, 2011AvailabilityAcademic Institutions and Nonprofits only -
pEMY01AB-PRO
Plasmid#127714PurposeIntegrative yeast promoter characterization plasmid. Clone in with BpiI. Has ChrXV homology upstream and an mVenus fragment downstream.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pEMY01CD-TER
Plasmid#127751PurposeIntegrative yeast terminator characterization plasmid. Clone in with BpiI. Has mVenus fragment upstream and a KanMX fragment downstream.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pJK430
Plasmid#155377PurposePphlF_A2NT in dCRv1, 2x cascade + [dCas9 + PA4-mVenus]DepositorInsertsdCas9 (bacteria)
PA4-mVenus
PphlF_A2NT in dCas9 sgRNA oscillator v1
UseCRISPRTagsExpressionBacterialMutationD10A H840A (catalytically inactive)PromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pJK520
Plasmid#155386PurposePphlF-TA1 crRNAs, 2x cascade. dCas12a (D917A) + PA4-mVenusDepositorInsertsdCas12a (F. novicida)
PA4-mVenus
PphlF-TA1 in dCas12a oscillator crRNAs
UseCRISPRTagsExpressionBacterialMutationD917A (nuclease-deactivating)PromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pGBKT7-GW
Plasmid#61703PurposeGateway compatible Y2H vector - BDDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceMay 4, 2015AvailabilityAcademic Institutions and Nonprofits only