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pDF0584 huDisCas7-11 S1006-GGGS-D1221 U6-NT guide

Plasmid

#186993

Purpose

AAV transgene plasmid for Cas7-11-S1006-GGGS-D1221, with U6 promoter-driven expression of non-targeting crRNA guide

Depositor

Jonathan Gootenberg, Omar Abudayyeh

Article

Kato et al Cell. 2022 May 25. pii: S0092-8674(22)00

Insert

cas7-11-s1006-gggs-d122, non-targeting crRNA guide

Use

AAV

Tags

Expression

Mutation

s1006-gggs-d1221 deletion

Promoter

Available since

Sept. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pEMS2284

Plasmid

#158419

Purpose

AAV plasmid with Ple349 (PDE6H MiniPromoter) driving expression of EmGFP. Contains WPRE.

Depositor

Elizabeth Simpson

Article

Korecki et al Gene Ther. 2021 Feb 2. pii: 10.1038/s414

Insert

Ple349 (PDE6H Human)

Use

AAV

Tags

Expression

Mutation

Promoter

Ple349

Available since

Feb. 4, 2021

Availability

Academic Institutions and Nonprofits only

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pEMS2214

Plasmid

#158417

Purpose

AAV plasmid with Ple265 (PCP2 MiniPromoter) driving expression of EmGFP. Contains WPRE.

Depositor

Elizabeth Simpson

Article

Korecki et al Gene Ther. 2021 Feb 2. pii: 10.1038/s414

Insert

Ple265 (PCP2 Human)

Use

AAV

Tags

Expression

Mutation

Promoter

Ple265

Available since

Feb. 4, 2021

Availability

Academic Institutions and Nonprofits only

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pZac2.1 U6-SaDMDR7-U6-SaDMDL2

Plasmid

#78603

Purpose

AAV vector containing gRNAs (for SaCas9) targeting Dmd introns 22 and 23

Depositor

Amy Wagers

Article

Tabebordbar et al Science. 2016 Jan 22;351(6271):407-11. d

Insert

U6-SaDMDR7-U6-SaDMDL2

Use

AAV and CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Oct. 27, 2017

Availability

Academic Institutions and Nonprofits only
pZac2.1 SV40-CMV-SaCas9-3xNLS

Plasmid

#78601

Purpose

AAV vector containing SaCas9

Depositor

Amy Wagers

Article

Tabebordbar et al Science. 2016 Jan 22;351(6271):407-11. d

Insert

SV40-CMV-SaCas9-3xNLS

Use

AAV and CRISPR

Tags

HA tag

Expression

Mammalian

Mutation

Promoter

SV40, CMV promoters

Available since

Dec. 2, 2017

Availability

Academic Institutions and Nonprofits only
pEMS2021

Plasmid

#49128

Purpose

AAV plasmid with DCX (Ple302) promoter driving expression of iCre.

Depositor

Elizabeth Simpson

Article

Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

Insert

ssAAV-Ple302-iCre

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

DCX

Available since

May 10, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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pEMS2271

Plasmid

#158418

Purpose

AAV plasmid with Ple341 (PCP2 MiniPromoter) driving expression of EmGFP. Contains WPRE.

Depositor

Elizabeth Simpson

Article

Korecki et al Gene Ther. 2021 Feb 2. pii: 10.1038/s414

Insert

Ple341 (PCP2 Human)

Use

AAV

Tags

Expression

Mutation

Promoter

Ple341

Available since

Feb. 4, 2021

Availability

Academic Institutions and Nonprofits only

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pDF0582 huDisCas7-11 R1045-GGGS-R1122 U6-NT guide

Plasmid

#186991

Purpose

AAV transgene plasmid for Cas7-11-R1045-GGGS-R1122, with U6 promoter-driven expression of non-targeting crRNA guide

Depositor

Jonathan Gootenberg, Omar Abudayyeh

Article

Kato et al Cell. 2022 May 25. pii: S0092-8674(22)00

Insert

cas7-11-r1045-gggs-r1122, non-targeting crRNA guide

Use

AAV

Tags

Expression

Mutation

r1045-gggs-r1122 deletion

Promoter

Available since

Sept. 6, 2022

Availability

Academic Institutions and Nonprofits only

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pEMS1995

Plasmid

#49125

Purpose

AAV plasmid with GPR88 (Ple94) promoter driving expression of iCre.

Depositor

Elizabeth Simpson

Article

Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

Insert

ssAAV-Ple94-iCre

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

GPR88

Available since

May 10, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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pEMS1997

Plasmid

#49127

Purpose

AAV plasmid with SLC6A4 (Ple198) promoter driving expression of iCre.

Depositor

Elizabeth Simpson

Article

Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

Insert

ssAAV-Ple198-iCre

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

SLC6A4

Available since

May 7, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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pEMS2143

Plasmid

#104356

Purpose

AAV plasmid with smCBA promoter driving expression of emerald GFP (EmGFP) reporter. Contains WPRE.

Depositor

Elizabeth Simpson

Article

Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

Insert

ssAAV-smCBA-EmGFP-WPRE

Use

AAV

Tags

Expression

Mutation

Promoter

smCBA

Available since

July 10, 2019

Availability

Academic Institutions and Nonprofits only

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pEMS2159

Plasmid

#70120

Purpose

Promoterless AAV plasmid with MCS to insert promoter for expression of iCre. Contains WPRE.

Depositor

Elizabeth Simpson

Article

Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

Insert

ssAAV-MCS-iCre-WPRE

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Promoterless

Available since

May 12, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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pEMS2031

Plasmid

#49134

Purpose

AAV plasmid with OLIG1 (Ple305) promoter driving expression of iCre.

Depositor

Elizabeth Simpson

Article

Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

Insert

ssAAV-Ple305-iCre

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

OLIG1

Available since

May 10, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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pZac2.1 U6-SaAi9L-U6-SaAi9R

Plasmid

#78604

Purpose

AAV vector containing gRNAs (for SaCas9) targeting Ai9 stop cassette

Depositor

Amy Wagers

Article

Tabebordbar et al Science. 2016 Jan 22;351(6271):407-11. d

Insert

gRNAs for SaCas9 targeting Ai9 locus

Use

AAV and CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Dec. 14, 2017

Availability

Academic Institutions and Nonprofits only
pEMS1996

Plasmid

#49126

Purpose

AAV plasmid with UGT8 (Ple267) promoter driving expression of iCre.

Depositor

Elizabeth Simpson

Article

Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

Insert

ssAAV-Ple267-iCre

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

UGT8

Available since

May 10, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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pEMS1977

Plasmid

#49109

Purpose

AAV plasmid with C8ORF46 (Ple251) promoter driving expression of iCre.

Depositor

Elizabeth Simpson

Article

Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

Insert

ssAAV-Ple251-icre

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

C8ORF46

Available since

May 10, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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pEMS2157

Plasmid

#70118

Purpose

Promoterless AAV plasmid with MCS to insert promoter for expression of EmGFP. Contains WPRE.

Depositor

Elizabeth Simpson

Article

Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

Insert

ssAAV-MCS-EmGFP-WPRE

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Promoterless

Available since

May 12, 2016

Availability

Academic Institutions and Nonprofits only

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pEMS2260

Plasmid

#158423

Purpose

AAV plasmid with Ple331 (PAX6 MiniPromoter) driving expression of EmGFP. Contains WPRE.

Depositor

Elizabeth Simpson

Article

Korecki et al Gene Ther. 2021 Feb 2. pii: 10.1038/s414

Insert

Ple331 (PAX6 Human)

Use

AAV

Tags

Expression

Mutation

Promoter

Ple331

Available since

Feb. 4, 2021

Availability

Academic Institutions and Nonprofits only

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pEMS1980

Plasmid

#49111

Purpose

Promoterless AAV plasmid with MCS to insert promoter for expression of iCre.

Depositor

Elizabeth Simpson

Article

Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

Insert

ssAAV-MCS-iCre

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Promoterless

Available since

May 10, 2016

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pEMS1993

Plasmid

#49123

Purpose

AAV plasmid with OLIG1 (Ple304) promoter driving expression of iCre.

Depositor

Elizabeth Simpson

Article

Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

Insert

ssAAV-Ple304-iCre

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

OLIG1

Available since

May 6, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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