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Plasmid#186291PurposeGateway entry vector for sgRNA (target 1: MpRTN1). Transient expression of sgRNA (Target 1: MpRTN1) for MpRTN1 in plant cellsDepositorInsertsgRNA-Target1
UseCRISPRTagsExpressionMutationPromoterAvailable sinceNov. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
PALI1_PIR_pFB1
Plasmid#191249PurposeExpresses PALI1 from insect cellsDepositorInsertLCoR
UseTagsMBPExpressionInsectMutationNAPromoterPolyhedrinAvailable sinceNov. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
LacZ_pHIVEGFP
Plasmid#191251PurposeExpresses LacZ in mammalian cells (used as a control)DepositorInsertLacZ
UseTagsEGFPExpressionMammalianMutationPromoterEF1alphaAvailable sinceNov. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
E42_gControl_dTet_IRES_mTurquoise2
Plasmid#189799PurposeRetroviral delivery of control guide RNADepositorInsertLuciferase gRNA
UseRetroviralTagsExpressionMutationPromoterAvailable sinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pYTK001_P2At(2nd)
Plasmid#183087Purpose2A peptide P2A cloned in yeast MoClo entry vector (pYTK001) for use as the second 2A peptide in tri-cistronic and third 2A peptide in quad-cistronic construct assemblyDepositorInsertP2A
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceOct. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
FnR8-10
Plasmid#182569PurposeHuman fibronectin fragment, type-III repeats 8-10, R1379A mutantDepositorInsertHuman fibronectin fragment, type-III repeats 8-10
UseTagsHis and TEV cleavage site after His tagExpressionBacterialMutationR1379APromoterAvailable sinceOct. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
hp-66%GC-40bp
Plasmid#169200PurposeForce-activating hairpin for 3'-to-5' helicaseDepositorInsert66%GC-40bp-hairpin
UseUnspecifiedTagsExpressionMutationPromoterAvailable sinceOct. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
hp-75%GC-20bp
Plasmid#169201PurposeForce-activating hairpin for 3'-to-5' helicaseDepositorInsert75%GC-20bp-hairpin
UseUnspecifiedTagsExpressionMutationPromoterAvailable sinceOct. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
hp-75%GC-40bp
Plasmid#169202PurposeForce-activating hairpin for 3'-to-5' helicaseDepositorInsert75%GC-40bp-hairpin
UseUnspecifiedTagsExpressionMutationPromoterAvailable sinceOct. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
hp-100%GC-20bp
Plasmid#169203PurposeForce-activating hairpin for 3'-to-5' helicaseDepositorInsert100%GC-20bp-hairpin
UseUnspecifiedTagsExpressionMutationPromoterAvailable sinceOct. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
hp-50%GC-Left-half-120bp
Plasmid#169204PurposeForce-activating hairpin for 3'-to-5' helicaseDepositorInsert50%GC-Left-half-120bp-hairpin
UseUnspecifiedTagsExpressionMutationPromoterAvailable sinceOct. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
hp-50%GC-Right-half-120bp
Plasmid#169205PurposeForce-activating hairpin for 3'-to-5' helicaseDepositorInsert50%GC-Right-half-120bp-hairpin
UseUnspecifiedTagsExpressionMutationPromoterAvailable sinceOct. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
hp-50-0-50%GC-Left-half-120bp
Plasmid#169206PurposeForce-activating hairpin for 3'-to-5' helicaseDepositorInsert50-0-50%GC-Left-half-120bp-hairpin
UseUnspecifiedTagsExpressionMutationPromoterAvailable sinceOct. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
hp-50-0-50%GC-Right-half-120bp
Plasmid#169207Purpose50-0-50%GC-Right-half-120bp-hairpinDepositorInsert50-0-50%GC-Right-half-120bp-hairpin
UseUnspecifiedTagsExpressionMutationPromoterAvailable sinceOct. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
hp-50%GC-20bp
Plasmid#169196PurposeForce-activating hairpin for 3'-to-5' helicaseDepositorInsert50%GC-20bp-hairpin
UseUnspecifiedTagsExpressionMutationPromoterAvailable sinceOct. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
hp-50%GC-40bp
Plasmid#169197PurposeForce-activating hairpin for 3'-to-5' helicaseDepositorInsert50%GC-40bp-hairpin
UseUnspecifiedTagsExpressionMutationPromoterAvailable sinceOct. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
hp-50%GC-40bp-Revbindsite
Plasmid#169198PurposeForce-activating hairpin for 5'-to-3' helicaseDepositorInsert50%GC-40bp-hairpin-binding-site-reversed
UseUnspecifiedTagsExpressionMutationPromoterAvailable sinceOct. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
hp-66%GC-20bp
Plasmid#169199PurposeForce-activating hairpin for 3'-to-5' helicaseDepositorInsert66%GC-20bp-hairpin
UseUnspecifiedTagsExpressionMutationPromoterAvailable sinceOct. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCRISPR_full-site
Plasmid#186277PurposeReporter plasmid for full-site selection assayDepositorInsertThiomicrospira leader repeat and modified chloramphenicol selection cassette (removed RBS and start codon))
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMpGE010-sgRNA-Target1 (MpRTN1)
Plasmid#186295PurposeBinary vector for CRISPR/Cas9 (target 1: MpRTN1) in plants (for Agrobacterium-mediated genetic transformation)DepositorInsertsgRNA-Target1
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceSept. 26, 2022AvailabilityAcademic Institutions and Nonprofits only