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pKG187
Plasmid#63077PurposeRetroviral expression of EGFP-TEV-S-tag FAAP24 for protein localization and affinity purification (LAP)DepositorInsertFAAP24, IMAGE clone 3659284 (FAAP24 Human)
UseRetroviralTagsEGFP and TEV-SExpressionMammalianMutationPromoterAvailable sinceJan. 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pOpen-Endonuclease VIII E. coli
Plasmid#165561PurposeBifunctional DNA glycosylase with DNA N-glycosylase and AP lyase activities; The N-glycosylase activity releases damaged pyrimidines, including thymine glycol and uracil glycol. The AP lyase activity cleaves DNA phosphodiester backbone at AP sites via beta and delta-elimination, creating a 1 nucleotide DNA gap with 5' and 3' phosphate termini.DepositorInsertEndonuclease VIII E. coli
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 16, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pIC84
Plasmid#63062PurposeRetroviral expression of EGFP-TEV-S-tag hCENP-H for protein localization and affinity purification (LAP)DepositorInserthCENP-H, IMAGE clone 3451767 (CENPH Human)
UseRetroviralTagsEGFP and TEV-SExpressionMammalianMutationPromoterAvailable sinceMarch 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
pIC146
Plasmid#63069PurposeRetroviral expression of EGFP-TEV-S-tag CENP-P for protein localization and affinity purification (LAP)DepositorInsertCENP-P, IMAGE clone 5554572 (CENPP Human)
UseRetroviralTagsEGFP and TEV-SExpressionMammalianMutationPromoterAvailable sinceMarch 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
R777-E030 Hs.CNKSR2-nostop
Plasmid#70314PurposeGateway ORF clone of human CNKSR2 [NM_001168647.1] without stop codon (for C-terminal fusions)DepositorInsertCNKSR2 (CNKSR2 Human)
UseGateway entry cloneTagsExpressionMutationPromoterAvailable sinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEB018
Plasmid#215568PurposeFuses insert of interest to an HA-tag, stop codon and S. commune Hom2 terminator, with flanks directing the construct to S. commune targeted integration locusDepositorInsert3 x Hemagglutinin (HA) Tag / Stop codon / Intron / Hom2-terminator
UseFungal homologous recombination plasmidTagsExpressionMutationPromoterAvailable sinceMay 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
F17-pAc5.1 EGFP Vasa 1-200
Plasmid#206484Purposevasa 1-200 expression for Schneider cell imagingDepositorInsertVasa 1-200
UseTagsEGFPExpressionInsectMutationPromoterAvailable sinceMay 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAW8_3HA
Plasmid#110233PurposeCre/Lox C-terminal tagging construct encoding 3 copies of the influenza virus hemagglutunin (HA) epitopeDepositorTypeEmpty backboneUseCre/LoxTagsExpressionMutationPromoterAvailable sinceAug. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
hC73S-pEGFP-C1
Plasmid#111540PurposeExpresses human KCNB1 C73S in mammalian cells for imagingDepositorInserthuman C73S-Kv2.1 (KCNB1 Human)
UseTagsEGFPExpressionMammalianMutationC73SPromoterCMVAvailable sinceJuly 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
hC73D-pEGFP-N1
Plasmid#111534PurposeExpresses human KCNB1 C73D in mammalian cells for imagingDepositorInserthuman C73D-Kv2.1 (KCNB1 Human)
UseTagsEGFPExpressionMammalianMutationC73DPromoterCMVAvailable sinceJuly 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
hC73A-pEGFP-C1
Plasmid#111539PurposeExpresses human KCNB1 C73A in mammalian cells for imagingDepositorInserthuman C73A-Kv2.1 (KCNB1 Human)
UseTagsEGFPExpressionMammalianMutationC73APromoterCMVAvailable sinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
hC73W-pEGFP-N1
Plasmid#111537PurposeExpresses human KCNB1 C73W in mammalian cells for imagingDepositorInserthuman C73W-Kv2.1 (KCNB1 Human)
UseTagsEGFPExpressionMammalianMutationC73WPromoterCMVAvailable sinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
hC73S-pEGFP-N1
Plasmid#111536PurposeExpresses human KCNB1 C73S in mammalian cells for imagingDepositorInserthuman C73S-Kv2.1 (KCNB1 Human)
UseTagsEGFPExpressionMammalianMutationC73SPromoterCMVAvailable sinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
hC73M-pEGFP-N1
Plasmid#111535PurposeExpresses human KCNB1 C73M in mammalian cells for imagingDepositorInserthuman C73M-Kv2.1 (KCNB1 Human)
UseTagsEGFPExpressionMammalianMutationC73MPromoterCMVAvailable sinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCG-Hc∆18
Plasmid#84817PurposeMeasles virus hemagglutinin expressing plasmidDepositorInsertMeasles virus H glycoprotein
UseTagsExpressionMammalianMutationPromoterCMV IEAvailable sinceFeb. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
GW1CMV-Perceval
Plasmid#21737Purposecan be used to monitor the ATP:ADP ratio during live-cell imagingDepositorInsertPerceval (fluorescent ATP:ADP sensor)
UseTags5'UTRExpressionMammalianMutationimproved Kozak sequencePromoterAvailable sinceAug. 5, 2009AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CMV-Myr-Pink Flamindo
Plasmid#204669PurposeMammalian expression of membrane-bound Pink Flamindo for cAMP imagingDepositorArticleInsertMyr-Pink Flamindo
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pIC144
Plasmid#63067PurposeRetroviral expression of EGFP-TEV-S-tag CENP-K for protein localization and affinity purification (LAP)DepositorInsertCENP-K, IMAGE clone 3511065 (CENPK Human)
UseRetroviralTagsEGFP and TEV-SExpressionMammalianMutationPromoterAvailable sinceJan. 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
APEX2 VLP
Plasmid#182211PurposeYeast integrative plasmid for expressing Murine polyomavirus deltaVP1 (GAL1 promoter) and APEX2 tagged with VP2C (GAL10 promoter).DepositorInsertsVP2C-APEX2
Murine polyomavirus deltaVP1 (NLS deletion mutant)
UseSynthetic Biology; Tem imagingTagsExpressionYeastMutationPromoterGAL1 and GAL10Available sinceMarch 25, 2022AvailabilityAcademic Institutions and Nonprofits only
