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Plasmid#194557PurposeMammalian Expression of mEGFP-HMGB1 Mutant (K184Rfs*44) Fusion Protein, "R > A" variantDepositorInsertHMGB1 (HMGB1 Human)
UseTagsmEGFPExpressionMammalianMutationR > A substitutions after Lys185PromoterAvailable sinceFeb. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRK5-mEGFP-HMGB1-MUT-Patchless
Plasmid#194553PurposeMammalian Expression of mEGFP-HMGB1 Mutant (K184Rfs*44) Fusion Protein, "Patchless" variantDepositorInsertHMGB1 (HMGB1 Human)
UseTagsmEGFPExpressionMammalianMutationK184Rfs*44, truncation by stop codon after Lys209PromoterAvailable sinceFeb. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pKT-IDH1(R132H)-IRES-Katushka
Plasmid#124257PurposeExpresses IDH1 with a R132H mutation and katushka fluorescent reporter. Construct has inverted repeats to be used in Sleeping Beauty transposon system.DepositorInsertIsocitrate dehydrogenase 1 (R132H) (IDH1 Human)
UseTagsExpressionMammalianMutationArginine 132 is mutated to Histidine. This mutati…PromoterAvailable sinceSept. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_ spike_del19
Plasmid#155297PurposeExpression of spike with C-term deletion of 19 aa, used to generate high efficiency SARS-CoV-2-pseudotyped lentiviral particlesDepositorInsertSARS-Cov2 spike_deleted (S SARS-Cov2)
UseGeneration of sars-cov-2-pseudotyped lentiviral p…TagsExpressionMutationDeletion in C-term 19 aa of spikePromoterCMVAvailable sinceAug. 5, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pTKA001
Plasmid#164577PurposeExpresses SARS-CoV-2 nsp7 from a pET46 backbone for IPTG-inducible bacterial expressionDepositorInsertnsp7 (ORF1ab )
UseTags6xHis, Ek, TEVExpressionBacterialMutationPromoterT7 PromoterAvailable sinceJan. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTKA002
Plasmid#164578PurposeExpresses SARS-CoV-2 nsp8 from a pET46 backbone for IPTG-inducible bacterial expressionDepositorInsertnsp8
UseTags6xHis, Ek, TEVExpressionBacterialMutationPromoterT7 PromoterAvailable sinceFeb. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
CRISPR-Cas9 Gateway System for Physcomitrella patens
Plasmid Kit#1000000151PurposeCRISPR-Cas9 Gateway System is a collection of 24 plasmids providing a modular, efficient CRISPR/Cas9 editing system for accurate and customizable repair in Physcomitrella patens.DepositorAvailable sinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTER_MDC1 (422-2)
Plasmid#22191DepositorInsertsh Mediator of DNA damage checkpoint 1
UseRNAiTagsExpressionMammalianMutationPromoterAvailable sinceJan. 8, 2010AvailabilityAcademic Institutions and Nonprofits only -
pENTR/pTER shMDC1 (441-1)
Plasmid#22192DepositorInsertsh Mediator of DNA damage checkpoint 1
UseRNAi; Entry vectorTagsExpressionMutationPromoterAvailable sinceDec. 17, 2009AvailabilityAcademic Institutions and Nonprofits only -
pQCXIP-F-luc
Plasmid#73058PurposeRetroviral expression of firefly luciferase for in vivo imagingDepositorInsertFirefly luciferase
UseRetroviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLenti X1 Zeo/pTER shMDC1 (673-1)
Plasmid#22193DepositorInsertsh Mediator of DNA damage checkpoint 1
UseLentiviral and RNAiTagsExpressionMutationPromoterAvailable sinceDec. 18, 2009AvailabilityAcademic Institutions and Nonprofits only -
pLenti X2 Zeo/pTER shMDC1 (764-1)
Plasmid#22194DepositorInsertshMediator of DNA damage checkpoint 1
UseLentiviral and RNAiTagsExpressionMutationPromoterAvailable sinceDec. 18, 2009AvailabilityAcademic Institutions and Nonprofits only -
cfSBFP2-N
Plasmid#110633PurposeCys-free blue fluorescent protein for extracellular imaging. SBFP2 variant (C48S/C70M). Molecular brightness is almost comparable to SBFP2.DepositorInsertcfSBFP2
UseTagsExpressionMutationCys48Ser/Cys70MetPromoterAvailable sinceApril 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV-mPlum-IFP fusion
Plasmid#49768PurposeFor near infrared in vivo optical imagingDepositorInsertmPlum-IFP
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 19, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLenti X2 Neo/pTER shMDC1 (w24-2)
Plasmid#22195DepositorInsertshMediator of DNA damage checkpoint 1
UseLentiviral and RNAiTagsExpressionMutationPromoterAvailable sinceDec. 15, 2009AvailabilityAcademic Institutions and Nonprofits only -
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cfSYFP2-N
Plasmid#110631PurposeCys-free yellow fluorescent protein for extracellular imaging. SYFP2 variant (C48S/C70M). Molecular brightness is almost comparable to SYFP2.DepositorInsertcfSYFP2-N
UseTagsMulti-cloning sitesExpressionMammalianMutationCys48Ser and Cys70/MetPromoterAvailable sinceApril 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
Stx3-Dendra2
Plasmid#82637PurposeExpresses fluorescent hybrid of syntaxin-3 for PALM imagingDepositorInsertSyntaxin-3 (Stx3 Rat)
UseTagsDendra2ExpressionMammalianMutationPromoterHCMVAvailable sinceSept. 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
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