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Plasmid#140200PurposeCloning vector for LbCas12a crRNAs ( Step 1 of the 2-step cloning alternative). Full PgpdA sequence is reconstituted when amplifying the expression cassette for cloning in a fungal vector.DepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyTagsdLbCas12a crRNA scaffoldExpressionMutationPromoterPgpdAAvailable sinceSept. 2, 2020AvailabilityAcademic Institutions and Nonprofits only
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1352wUV2 F1bbs kan GCGTGGNNN
Plasmid#18753DepositorInsert3 tandem zinc fingers
UseTagsExpressionBacterialMutationA Kan cassette (1090bp) is inserted between two b…PromoterAvailable sinceJuly 11, 2008AvailabilityAcademic Institutions and Nonprofits only -
pGL4.13/GUG-FFLuc-3XFLAG
Plasmid#127334PurposeExpress 3xFLAG tagged firefly luciferase (FFLuc) from GUG start codonDepositorInsertFirefly luciferase
UseTags3xFLAGExpressionMammalianMutationPromoterSV40Available sinceAug. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMaCTag-Z29
Plasmid#120072PurposeTemplate for C-terminal PCR tagging of mammalian genes (Zeocin selection) with Strep-IIDepositorInsertStrep-II
UsePcr templateTagsStrep-IIExpressionMutationPromoterNoneAvailable sinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
p413GAL1-Zipp.-L-IFP-F[2] (Yeast)
Plasmid#52893PurposePlasmid+insert encoding GCN4-leucine zipper fused to IFP-F[2] by the linker (GGGGS) X 2. Zipper-L-IFP-F[2] (XbaI/XhoI). Zipper is (XbaI /ClaI). Plasmid confers ampicillin resistance to DH5α.DepositorInsertZipp.-L-IFP-F[2]
UseTagsL-IFP-F[2]ExpressionYeastMutationPromoterGAL1Available sinceJune 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
SEPT11 sh1 shRNA
Plasmid#122020PurposeKnock Down of Septin 11. It targetes Septin 11 mRNA. It knocks down all known splice variants of Sept 11 mRNAs.DepositorInsertSEPT11 shRNA (Sept11 Rat)
UseRNAiTagsExpressionMutationPromoterAvailable sinceFeb. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCPP6450
Plasmid#128731PurposePhytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) Gateway donor cloneDepositorInsertHopAD1
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFastBac1-OSF-TRIM5alpha(rhesus)
Plasmid#79033PurposeBaculoviral entry vector to produce Bac-to-bac baculovirusesDepositorInsertOSF-TRIM5alpha(rhesus)
UseTagsOneSTrEP-FLAGExpressionInsectMutationPromoterpolyhedrinAvailable sinceJan. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMRE-Tn5-164
Plasmid#118544PurposeminiTn5 plasmid to deliver constitutively expressed fluorescent protein genes in bacteriaDepositorInsertmOrange2
UseMinitn5 delivery vectorTagsExpressionBacterialMutationPromoterAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
p3XFlag-CMV10-Flag-mMcl-1 KR
Plasmid#32979DepositorInsertMcl-1 (Mcl1 Mouse)
UseTags3XFlagExpressionMammalianMutationAll lys changed to ArgPromoterCMVAvailable sinceMarch 15, 2012AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-CAML-Myc-His
Plasmid#22295DepositorInsertcalcium modulating ligand (CAMLG Human)
UseTagsMyc-HisExpressionMammalianMutationPromoterAvailable sinceDec. 2, 2009AvailabilityAcademic Institutions and Nonprofits only -
pMRE-Tn5-166
Plasmid#118546PurposeminiTn5 plasmid to deliver constitutively expressed fluorescent protein genes in bacteriaDepositorInsertmCardinal
UseMinitn5 delivery vectorTagsExpressionBacterialMutationPromoterAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
mVenus-NES-SspB-Tiam-DHPH-P2A-iLIDcaax
Plasmid#173865PurposeMammalian expression of mVenus-NES-SspB-Tiam-DHPH-P2A-iLIDcaax (opto-Rac1)DepositorInsertmVenus-NES-SspB-Tiam-DHPH-P2A-iLIDcaax
UseTagsC-terminal CAAX-box and mVenusExpressionMammalianMutationPromoterCMVAvailable sinceSept. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pNH-TrxT-G-L11
Plasmid#127831PurposeProduction of recombinant human ribosomal protein L11DepositorInsertRPL11 (RPL11 Human)
UseTagsHis6 - Thioredoxin - TEVExpressionBacterialMutationPromoterT7Available sinceAug. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pIS1-wt RhoA 3'UTR
Plasmid#26089DepositorInsertwt RhoA 3'UTR (RHOA Human)
UseLuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 20, 2010AvailabilityAcademic Institutions and Nonprofits only -
pGL2-Skp2 promoter-luciferase
Plasmid#81119PurposeReporter activity of SKP2 gene promoterDepositorInsertSKP2 (Skp2 Mouse)
UseLuciferaseTagsluciferaseExpressionMutationcontains the region from 1_kb upstream of transcr…PromoterSKP2Available sinceSept. 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
mOrange2-Vinculin-23
Plasmid#57978PurposeLocalization: Focal Adhesions, Excitation: 549, Emission: 565DepositorInsertVinculin (VCL Human)
UseTagsmOrange2ExpressionMammalianMutationV234L in vinculinPromoterCMVAvailable sinceOct. 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSB165 - pL1R3_pMAS::rLUC-I::tMAS
Plasmid#123198Purposebinary plant vector for transient expression of a Renilla luciferase (rLUC, with intron)DepositorInsertpMAS::rLUC-I::tMAS
UseLuciferaseTagsExpressionPlantMutationPromoterAvailable sinceMay 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMV-HA-mIFITM3-K83A
Plasmid#58401PurposeExpresses murine IFITM3-K83A with an N-terminal HA tag in mammalian cellsDepositorInsertIFITM3 (Ifitm3 Mouse)
UseTagsHAExpressionMammalianMutationLysine 83 to AlaninePromoterCMV IEAvailable sinceSept. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCMV-HA-mIFITM3-K24A
Plasmid#58400PurposeExpresses murine IFITM3-K24A with an N-terminal HA tag in mammalian cellsDepositorInsertIFITM3 (Ifitm3 Mouse)
UseTagsHAExpressionMammalianMutationLysine 24 to AlaninePromoterCMV IEAvailable sinceSept. 4, 2014AvailabilityAcademic Institutions and Nonprofits only