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Plasmid#209077PurposeNgn2 mediated cortical neuron induction, dox induction is includedDepositorInsertsUseTagsExpressionMammalianMutationPromoterTRE4GAvailable sinceDec. 5, 2023AvailabilityAcademic Institutions and Nonprofits only
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pETDuet-1 Hepatitis C Virus NS3/NS4A
Plasmid#61696Purposeinducible dual expression of NS3 protease along with its cofactor NS4ADepositorInsertsNS4A
NS3
UseTagshisExpressionBacterialMutationPromoterT7Available sinceApril 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
FLHKI-pGFPN3
Plasmid#21917DepositorInsertFull length human HKI in pGFP-N3 (HK1 Human)
UseTagsGFPExpressionMammalianMutationThis is full length human HKI in pGFP-N3PromoterAvailable sinceSept. 17, 2009AvailabilityAcademic Institutions and Nonprofits only -
pU6-Sp-gRNA-CCR5_DF_B584b_attB
Plasmid#182148PurposeTo insert attB attachment site at CCR5 in human cells via twinPEDepositorInsertCCR5-B584b-attB pegRNA
UseTagsExpressionMammalianMutationPromoterhU6Available sinceApril 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
T7-VEE-OCT4-t2a-KLF4-e2a-SOX2-17-IRES-GLIS1
Plasmid#193356PurposeVEE-OKS*iG (self-replicating RNA vector expressing human OCT4 + KLF4 + super-SOX + GLIS1 + PuroR) for generation of integration-free iPSCsDepositorUseVenezuelan equine encephalitis (vee) virus rna re…TagsExpressionMammalianMutationSOX2-17 is engineered highly cooperative chimeric…PromoterAvailable sinceDec. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pU6-Sp-gRNA-CCR5_DF_A509b_attB
Plasmid#182147PurposeTo insert attB attachment site at CCR5 in human cells via twinPEDepositorInsertCCR5-A509b-attB pegRNA
UseTagsExpressionMammalianMutationPromoterhU6Available sinceMarch 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
AAV-CamKIIa-jGCaMP8s-WPRE (AAV Retrograde)
Viral Prep#176752-AAVrgPurposeReady-to-use AAV Retrograde particles produced from AAV-CamKIIa-jGCaMP8s-WPRE (#176752). In addition to the viral particles, you will also receive purified AAV-CamKIIa-jGCaMP8s-WPRE plasmid DNA. CamKIIa-driven expression of calcium sensor GCaMP8s. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.DepositorPromoterCamKIIalphaTagsNoneAvailable sinceJuly 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDonor_ShCAST_kanR
Plasmid#127924PurposeKanamycin resistance donor for ShCASTDepositorInsertKanR, R6K origin of replication
UseTagsExpressionMutationPromoterAvailable sinceAug. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEGFP rbFOX2
Plasmid#63086PurposeMammalian expression of EGFP tagged FOX2DepositorInsertRBFOX2 (RBFOX2 Human)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceApril 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pJJiaDEST-APEX2-GAL3
Plasmid#149518PurposeExpress APEX2-Galectin-3 in mammalian cellsDepositorInsertLGALS3 (LGALS3 Human)
UseTagsAPEX2ExpressionMammalianMutationPromoterCMVAvailable sinceAug. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pVoPo-04
Plasmid#187839PurposeSuicide vector to generate markerless gene deletions in Fusobacterium nucleatumDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28a hMDH1v3 (+TEV)
Plasmid#204229PurposeBacterial expression of human cytosolic malate dehydrogenase isoform 1 transcript variant 3 (hMDH1v3); Use in CURES (integration of research into undergraduate teaching labs).InserthMDH1v3 (MDH1 Human)
UseTags6X HisExpressionBacterialMutationInserted a G after ATG to maintain kozak sitePromoterLacAvailable sinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHelper_ShCAST_sgRNA
Plasmid#127921PurposeExpresses ShCAST and an empty sgRNA scaffold. New targets can be added using Golden Gate assembly (LguI sites).DepositorInsertShTnsB, ShTnsC, ShTniQ, ShCas12k, sgRNA scaffold for guide cloning (LguI sites)
UseTagsExpressionMutationPromoterAvailable sinceJuly 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
miABE
Plasmid#205412PurposeVector encoding human codon-optimized enhanced-activity nOgeuIscB fusion with TadA8e driven by CAG promoter, optimized _RNA (_RNA*) driven by hU6, and mCherry driven by CMV promoterDepositorInsertpU6-_RNA*-pCAG-bpNLS-TadA8e(V106W)-32aa-OgeuIscB*(D61A)-GS-bpNLS-GS-TadA8e(V106W)-bpNLS-bGH polyA-pCMV-mCherry-AmpR
UseTagsExpressionMammalianMutationD61A+E85R+H369R+S387R+S457RPromoterhU6, CAG, CMVAvailable sinceDec. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDisCoTune
Plasmid#173713PurposePromotes expression of disulfide-rich protein in E. coli T7 expression system. The plasmid encodes expression of the folding factors Erv1p and hPDI controlled by the Ptac promoter.DepositorInsertsUseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
UPB-colon (UPB4)
Plasmid#180786PurposeUnique Projection Barcode (UPB4)-Colon for Projection-seqDepositorInserthM3Dq ( partial )
UseAAVTagsExpressionMammalianMutationPromoterCAGAvailable sinceAug. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
N-WASP(CRIB)-mScarlet-I-N-WASP(CRIB)
Plasmid#191451PurposeVisualizing endogenous & active Cdc42DepositorInsertN-WASP
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-MS2-HA-HsStaufen2-T1_V
Plasmid#147741PurposeMammalian Expression of HsStaufen2-T1DepositorInsertHsStaufen2-T1 (STAU2 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHR_Gal4UAS_tBFP_PGK_mCitrine
Plasmid#188386PurposeLentiviral vector - tBFP reporter for Gal4-based SNIPRs with a constitutive mCitrine;DepositorInsertGal4UAS_tBFP_PGK_mCitrine
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMarch 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCE-hOCT3/4
Plasmid#41813PurposeNon-integrating (episomal) expression of human OCT3/4DepositorInsertPOU5F1 (POU5F1 Human)
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceApril 9, 2013AvailabilityAcademic Institutions and Nonprofits only