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Showing: 5181 - 5200 of 14973 results

  2. lentiCRISPRv2-sgRNAs-del-ZNF91-mcherry

    Plasmid
    #202822
    Purpose
    Lentiviral vector modified to express two sgRNAs that delete exon 1 within the ZNF91 gene with EF-1apha for Cas9 and mcherry expression
    Depositor
    Matthew Anderson
    Article
    Nadler et al Commun Biol. 2023 Mar 30;6(1):347. doi:
    Insert
    two sgRNAs that delete exon 1 within the ZNF91 gene
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6 - two
    Available since
    July 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    202822-plasmid-map-sequence-id-399648

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  10. sgRNA(MS2)_zeo insert c-MYC

    Plasmid
    #164636
    Purpose
    sgRNA(MS2)_zeo backbone with cloned target sequence for human c-MYC. The insert sequence is: 5-CACCGTTCCCCCACGCCCTCTGCTT-3´ . This sgRNA achieves an upregulation of MYC in combination with dCAS9-VP64
    Depositor
    Günter Schneider
    Insert
    target sequence for c-Myc activation (MYC Human)
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6 and EF1A
    Available since
    May 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    164636-plasmid-map-sequence-id-326094

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  11. pHiFi Cas9-2×sgRNA (empty, donor)

    Plasmid
    #162277
    Purpose
    All-in-one vector for CRISPR/Cas9-mediated homology-independent knock-in system. The plasmid contains BPNLS-HiFi Cas9-BPNLS and two sgRNA expression cassettes.
    Depositor
    Kazuhisa Nakayama
    Article
    Fujisawa et al Biol Open. 2021 Aug 27. pii: 271969. doi
    Insert
    HiFi Cas9
    Use
    CRISPR
    Tags
    BPNLS and FLAG tag
    Expression
    Mammalian
    Mutation
    SpCas9 (R691A)
    Promoter
    Available since
    Aug. 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    162277-plasmid-map-sequence-id-319794

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  12. pDD122 (Peft-3::Cas9 + ttTi5605 sgRNA)

    Plasmid
    #47550
    Purpose
    Cas9 + sgRNA plasmid that is targeted to a genomic site near the ttTi5605 Mos1 insertion allele
    Depositor
    Bob Goldstein
    Article
    Dickinson et al Nat Methods. 2013 Sep 1. doi: 10.1038/nm
    Inserts
    Cas9
    ttTi5605 sgRNA
    Use
    CRISPR
    Tags
    HA and NLS
    Expression
    Worm
    Mutation
    Codon optimized and with synthetic introns for C.…
    Promoter
    U6 and eef-1A.1 (eft-3)
    Available since
    Sept. 9, 2013
    Availability
    Academic Institutions and Nonprofits only
    47550-plasmid-map-sequence-id-67865

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  13. pShCAST(Cas12k-TnsC)-sgRNA_entry (CJT28)

    Plasmid
    #181789
    Purpose
    Expresses 3-component ShCAST containing a Cas12k-TnsC fusion. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).
    Depositor
    Benjamin Kleinstiver
    Article
    Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038
    Insert
    ShTnsB, ShTniQ, ShCas12k-ShTnsC
    Use
    Tags
    Expression
    Bacterial
    Mutation
    nAniI = K227M, F80K, L232K
    Promoter
    Lac and J23119
    Available since
    March 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    181789-plasmid-map-sequence-id-354816

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  17. pKSB-618 U6-sgRNA2/3 GGAA AACA

    Plasmid
    #173674
    Purpose
    To clone and express a gRNA in Anopheles
    Depositor
    Eric Marois
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Insect
    Mutation
    Promoter
    Available since
    Sept. 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    173674-plasmid-map-sequence-id-342962

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Showing: 5181 - 5200 of 14973 results