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Plasmid#223948PurposeGateway cloning of genes of interest with an N-terminal mCherry fusion; generation of stable cells with doxycycline inducible expressionDepositorTypeEmpty backboneUseTagsmCherryExpressionMammalianMutationPromoterAvailable sinceOct. 31, 2024AvailabilityAcademic Institutions and Nonprofits only
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pcDNA5-FRT-TOgw-N-mEGFP
Plasmid#223949PurposeGateway cloning of genes of interest with an N-terminal mEGFP fusion; generation of stable cells with doxycycline inducible expressionDepositorTypeEmpty backboneUseTagsmEGFPExpressionMammalianMutationPromoterAvailable sinceOct. 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBP-R_dummy-RBS
Plasmid#204081PurposeType 0 RBS partDepositorInsertDummy sequence
UseTagsExpressionBacterialMutationNonePromoterAvailable sinceJune 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBP-C_ccdB
Plasmid#204114PurposeType 0 CDS partDepositorInsertToxic DNA gyrase inhibitor ccdB
UseTagsExpressionBacterialMutationNonePromoterAvailable sinceJan. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBP-C_mTagBFP2
Plasmid#204109PurposeType 0 CDS partDepositorInsertmTagBFP2 blue fluorescent protein
UseTagsExpressionBacterialMutationNonePromoterAvailable sinceJan. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBP-C_dummy-CDS
Plasmid#204108PurposeType 0 CDS partDepositorInsertDummy sequence
UseTagsExpressionBacterialMutationNonePromoterAvailable sinceJan. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBP-P_dummy-promoter
Plasmid#204069PurposeType 0 promoter partDepositorInsertDummy sequence
UseTagsExpressionBacterialMutationNonePromoterAvailable sinceJan. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGL2_213
Plasmid#210697PurposeEven ORI-marker screen destination vector; contains sfYFP dropout for barcoded-ORI insertionDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL2_212
Plasmid#210696PurposeEven ORI-marker screen destination vector; contains sfYFP dropout for barcoded-ORI insertionDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL2_211
Plasmid#210695PurposeEven ORI-marker screen destination vector; contains sfYFP dropout for barcoded-ORI insertionDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBP-C_NanoLuc
Plasmid#204112PurposeType 0 CDS partDepositorInsertNanoLuc luciferase
UseTagsExpressionBacterialMutationNonePromoterAvailable sinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBP-T_dummy-term
Plasmid#204115PurposeType 0 terminator partDepositorInsertDummy sequence
UseTagsExpressionBacterialMutationNonePromoterAvailable sinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBP-T_T7-term
Plasmid#204124PurposeType 0 terminator partDepositorInsertT7 terminator
UseTagsExpressionBacterialMutationNonePromoterAvailable sinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBP-C_sfGFP
Plasmid#204111PurposeType 0 CDS partDepositorInsertsfGFP green fluorescent protein
UseTagsExpressionBacterialMutationNonePromoterAvailable sinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDEST-Spe4-Kozak-emiRFP703-RfA
Plasmid#186376PurposeDestination vector for the expression of a fluorescent fusion of N-ter emiRFP703 and a protein of interest into embryos, by mRNA microinjectionDepositorTypeEmpty backboneUseGateway destination vectorTagsExpressionMutationPromoterAvailable sinceJune 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDEST-Spe4-RfA-mVenus-stop
Plasmid#186394PurposeDestination vector for the expression of a fluorescent fusion of a protein of interest with C-ter mVenus into embryos, by mRNA microinjectionDepositorTypeEmpty backboneUseGateway destination vectorTagsExpressionMutationPromoterAvailable sinceMay 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDEST-Spe4-Kozak-miRFP713-RfA
Plasmid#186381PurposeDestination vector for the expression of a fluorescent fusion of N-ter miRFP713 and a protein of interest into embryos, by mRNA microinjectionDepositorTypeEmpty backboneUseGateway destination vectorTagsExpressionMutationPromoterAvailable sinceMay 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDEST-Spe4-RfA-mNeonGreen-stop
Plasmid#186392PurposeDestination vector for the expression of a fluorescent fusion of a protein of interest with C-ter mNeonGreen into embryos, by mRNA microinjectionDepositorTypeEmpty backboneUseGateway destination vectorTagsExpressionMutationPromoterAvailable sinceOct. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST-Spe4-RfA-miRFP713-stop
Plasmid#186391PurposeDestination vector for the expression of a fluorescent fusion of a protein of interest with C-ter miRFP713 into embryos, by mRNA microinjectionDepositorTypeEmpty backboneUseGateway destination vectorTagsExpressionMutationPromoterAvailable sinceOct. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST-Spe4-RfA-mEos4b-stop
Plasmid#186390PurposeDestination vector for the expression of a fluorescent fusion of a protein of interest with C-ter mEos4b into embryos, by mRNA microinjectionDepositorTypeEmpty backboneUseGateway destination vectorTagsExpressionMutationPromoterAvailable sinceOct. 4, 2022AvailabilityAcademic Institutions and Nonprofits only