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Plasmid#162460PurposeYeast display plasmid for expression of C-terminal fusions with Aga2p yeast agglutinin. The reporter DnbALFA was fused to the N-terminus of Aga2p.DepositorTypeEmpty backboneUseShuttle vector bacteria/yeastTagsDnbALFA designed ALFA nanobody, HA tag, HDEL sequ…ExpressionMutationPromoterAvailable sinceApril 9, 2021AvailabilityAcademic Institutions and Nonprofits only
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AAVS1-TRE3G-NODAL
Plasmid#115638PurposeFor targeted integration and inducible expression of human NODAL using doxycyclineDepositorInsertNODAL (NODAL Human)
UseTagsExpressionMammalianMutationPromoterTRE3GAvailable sinceSept. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJYDN3
Plasmid#162456PurposeYeast display platform plasmid N3 which allows for simultaneous expression of the protein of interest at the N-terminus of Aga2p yeast agglutinin and eUnaG2 or nbALFA detection.DepositorTypeEmpty backboneUseShuttle vector bacteria/yeastTagsALFA tag, HA, myc tags, and eUnaG2 fluorescent pr…ExpressionMutationPromoterAvailable sinceFeb. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
ttAtCas12a+int
Plasmid#182384PurposeGives very high editing efficiency in barley. Also works in Brassica oleraceaDepositorInsertLbCas12a coding sequence with D156R and Arabidopsis introns
UseCRISPRTagsExpressionMutationD156R + 8 Arabidopsis introns addedPromoterNoneAvailable sinceJuly 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pH6HTC_TKT-Halo
Plasmid#175331PurposeProduction of HaloTag fusion proteinDepositorInsertTKT (TKT Human)
UseCell free expressionTagsHaloTag-6xHisExpressionBacterialMutationPromoterAvailable sinceOct. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCxcl1-Firefly
Plasmid#163896PurposeLuciferase reporter for mouse Cxcl1DepositorInsertLuciferase
UseLuciferaseTagsExpressionMutationPromoterCxcl1Available sinceJan. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJYDN2
Plasmid#162454PurposeYeast display plasmid intended for co-cultivation labeling by using designed ALFA-tag binding nanobody (DnbALFA) and expression of N-terminal fusions with Aga2p yeast agglutinin.DepositorTypeEmpty backboneUseShuttle vector bacteria/yeastTagsDnbALFA nanobody and Ha and mycExpressionMutationPromoterAvailable sinceFeb. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCD86-mEGFP
Plasmid#190745PurposeExpression of mEGFP-tagged CD86 in mammalian cells.DepositorInsertCD86 B7-2, B7.2, B70, CD28LG2, LAB (CD86 Human)
UseTagsmEGFPExpressionMammalianMutationPromoterCMVAvailable sinceSept. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-OTI-TCRB_p2_TCRA
Plasmid#131034PurposeExpresses OT-I TCRB linked to TCRA with the P2A self-cleaving peptideDepositorArticleUseTagsExpressionMammalianMutationPromoterCMV/T7Available sinceOct. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.1_CoumRS
Plasmid#64882PurposeExpresses Coumarinyl- tRNA synthetaseDepositorInsertCoum_RS
UseTagsFlagExpressionMutationPromoterCMVAvailable sinceJan. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJMH147
Plasmid#186647PurposeaadA.co-TaadA-J23107-4ABH.co.rm1-PpsaA-nhoA.co.rm1 between homology arms for pilADepositorArticleInsertaadA.co-TaadA-J23107-4ABH.co.rm1-PpsaA-nhoA.co.rm1
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJan. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJYDN2p
Plasmid#162455PurposePositive control plasmid for co-cultivation labeling by using designed ALFA-tag binding nanobody (DnbALFA).DepositorInsertAga2p-DnbALFA (AGA2 )
UseShuttle vector bacteria/yeastTagsDnbALFA nanobody and HA and mycExpressionMutationPromoterGAL1Available sinceFeb. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGL0_142 [EilR-PjexD]
Plasmid#199026PurposeLevel 0 partDepositorInsertEilR-PjexD
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJune 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCWU6-xylR-E-lepB
Plasmid#190725Purposefine tune expression of type 1 signal peptidase under the control of xylose- and theophylline- inducible systemDepositorInsertxylR-E-lepB
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pKB225
Plasmid#170015PurposeEncodes LacI-controlled expression of surface display protein with no C-terminal peptide and PvirK-mNeonGreen reporterDepositorArticleInsertsmNeonGreen
Peptide display protein with no C-terminal peptide
UseSynthetic BiologyTagsLpp, OmpA, and TetherExpressionBacterialMutationPromoterPtac and PvirKAvailable sinceNov. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTH849-gst-optec
Plasmid#89590PurposePlasmid for bacterial expression of His-tagged GST encoded by preferred codonsDepositorArticleInsertglutathione-S transferase codon-optimised for bacterial expression
UseTags8xhistidineExpressionBacterialMutationPromoterT7Available sinceMarch 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGL2_136
Plasmid#199083PurposeEven ORI-marker screen destination vector with sfYFP dropout for barcoded-ORI insertionDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJune 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL2_137
Plasmid#199084PurposeEven ORI-marker screen destination vector with sfYFP dropout for barcoded-ORI insertionDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJune 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSABEd
Plasmid#133968PurposeExpresses ABEd CRISPR base editor and gRNA scaffold in StreptomycesDepositorArticleInsertABEd
UseCRISPR and Synthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pYCTK019
Plasmid#176723PurposeThe plasmid is part of a GG toolkit for cell-cell communication in S. cerevisiae. This plasmid is an L0 part plasmid and contains the Ste2 gene of Candida albicans (receiver part).DepositorArticleInsertste2Ca
UseSynthetic BiologyTagsExpressionMutationCodon-optimized for expression in S. cerevisiaePromoterAvailable sinceJan. 6, 2022AvailabilityAcademic Institutions and Nonprofits only