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Showing: 501 - 520 of 158672 results

  2. prhaBAD-pA-Hia5

    Plasmid
    #222303
    Purpose
    Plasmid for rhamnose inducible expression and purification of protein A-Hia5-6XHis (pA-Hia5) for antibody directed DNA (adenine) methylation (as in DiMeLo-seq)
    Depositor
    Aaron Straight
    Article
    Altemose et al Nat Methods. 2022 Jun;19(6):711-723. doi
    Insert
    pA-Hia5
    Use
    Tags
    6His and protein A
    Expression
    Bacterial
    Mutation
    codon optimized for bacterial expression
    Promoter
    rhaBAD
    Available since
    Oct. 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    222303-plasmid-map-sequence-id-447746

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  7. pOpen-MMLV_RT (mut H)

    Plasmid
    #165546
    Purpose
    Single 75 kDa monomer, cDNA synthesis; high enzyme activity and processivity.
    Depositor
    Jennifer Molloy, FreeGenes Project, Drew Endy
    Insert
    Moloney Murine Leukemia Virus (MMLV) Reverse Transcriptase (RNAse H deactivated by 3 mutations)
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 10, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165546-plasmid-map-sequence-id-339423

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  8. pAB1677 REPAIR.t3

    Plasmid
    #176320
    Purpose
    CMV-HIVNES-GS-dCas13bt3- (GGS)2-huADAR2dd(E488Q) (REPAIR.t3 expression)
    Depositor
    Feng Zhang
    Article
    Kannan et al Nat Biotechnol. 2021 Aug 30. pii: 10.103
    Inserts
    human codon optimized Cas13bt3 (catalytically inactivated)
    huADAR2dd(E488Q) (ADARB1 Human)
    Use
    CRISPR
    Tags
    HIV NES
    Expression
    Mammalian
    Mutation
    E488Q, only the deaminase domain (aa 276-702 are …
    Promoter
    CMV
    Available since
    Oct. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    176320-plasmid-map-sequence-id-345287

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  10. pARC8-LambdaRedBeta-EcSSB-EcMutLE32K

    Plasmid
    #162576
    Purpose
    Arabinose inducible recombineering plasmid encoding Lambda-Red Beta with EcSSB for use with dsDNA templates and enhanced recombination efficiency. Contains a dominant negative mismatch repair protein.
    Depositor
    George Church
    Article
    Filsinger et al Nat Chem Biol. 2021 Jan 18. pii: 10.1038
    Inserts
    Lambda Red-Beta
    E. coli SSB
    EcMutLE32K
    Use
    Tags
    Expression
    Bacterial
    Mutation
    E32K mutated to give dominant negative phenotype
    Promoter
    pBAD
    Available since
    May 22, 2021
    Availability
    Academic Institutions and Nonprofits only
    162576-plasmid-map-sequence-id-326400

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  16. pAAV-CAG-2xNLS-tdTomato-WPRE-hGHpolyA (AAV9)

    Viral Prep
    #192552-AAV9
    Purpose
    Ready-to-use AAV9 particles produced from pAAV-CAG-2xNLS-tdTomato-WPRE-hGHpolyA (#192552). In addition to the viral particles, you will also receive purified pAAV-CAG-2xNLS-tdTomato-WPRE-hGHpolyA plasmid DNA. CAG-driven expression of nuclear localized tdTomato. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Guoping Feng
    Promoter
    CAG
    Tags
    tdTomato
    Available since
    June 26, 2024
    Availability
    Academic Institutions and Nonprofits only
Showing: 501 - 520 of 158672 results