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Plasmid#172985PurposeCRISPRi for TAOK2DepositorInsertsgTAOK2i (TAOK2 Human)
UseCRISPR and LentiviralTags3xFLAGExpressionMutationPromoterAvailable sinceOct. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pINDUCER21-puro_TAOK2-D151A
Plasmid#172980Purposedoxycycline-inducible expression of kinase-dead TAOK2DepositorInsertkinase-dead TAOK2 (TAOK2-D151A) (TAOK2 Human)
UseLentiviralTags3xFLAGExpressionMammalianMutationD151APromoterAvailable sinceOct. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLV_dCas9-ZIM3-KRAB_sgTAOK2i_#1
Plasmid#172983PurposeCRISPRi for TAOK2DepositorInsertsgTAOK2i (TAOK2 Human)
UseCRISPR and LentiviralTags3xFLAGExpressionMutationPromoterAvailable sinceOct. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET19-TdTdelta5cys
Plasmid#207465PurposeBacterial expression of Terminal deoxynucleotidyl transferase (TdT) surface-cysteine free variantDepositorInsertTdTdelta5cys (Dntt Mouse)
UseTags10xHisExpressionBacterialMutationCys188Ala, Cys216Ser, Cys302Ala, Cys378Ala, and C…PromoterAvailable sinceSept. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcmv3-IRG1-L272Q-HA
Plasmid#198184PurposeMammalian expression of HA-tagged human ACOD1 (IRG1) L272QDepositorInsertACOD1 (ACOD1 Human)
UseTagsHAExpressionMammalianMutationThe leucine in IRG1 at position 272 mutates to gl…PromoterCMVAvailable sinceApril 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcmv3-IRG1-H277Y-HA
Plasmid#198185PurposeMammalian expression of HA-tagged human ACOD1 (IRG1) H277YDepositorInsertACOD1 (ACOD1 Human)
UseTagsHAExpressionMammalianMutationThe histidine in IRG1 at position 277 mutates to …PromoterCMVAvailable sinceApril 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pXD70DAmt1-DadR/DadS-Pdadh-DadhABC(R506S, A2)
Plasmid#191631PurposeE. coli - Eggerthella lenta shuttle plasmid (KanR), native promoter-dopamine dehydroxylase from dopamine-metabolizing E. lenta A2 strain (R506S mutation) with transcriptional regulators DadR/DadSDepositorInsertDopamine dehydroxylase (R506S mutation, inactive form)
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pXD71Cas10.0RTcgr-Pct5.1-crRNA(AarI)-RT(Δcgr12)
Plasmid#191651PurposeE. coli - Eggerthella lenta shuttle plasmid (KanR), cumate-inducible promoter Pct5.1-crRNA(nontargeting spacer) cgr1/cgr2-deleting repair template, used as negative control for pXD71Cas10.4RT4DepositorInsertCymR
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1/V5-HisA-RvCAHS8-GS-AcGFP1
Plasmid#192470PurposeTransient expression of RvCAHS8 (fly codon optimized)-GS_linker-AcGFP1 in Drosophila cellsDepositorInsertCAHS8
UseTagsAcGFP1ExpressionInsectMutationPromoterAc5 promoterAvailable sinceDec. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1/V5-HisA-RvCAHS3-GS-AcGFP1
Plasmid#192469PurposeTransient expression of RvCAHS3 (fly codon optimized)-GS_linker-AcGFP1 in Drosophila cellsDepositorInsertCAHS3
UseTagsAcGFP1ExpressionInsectMutationPromoterAc5 promoterAvailable sinceDec. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEThT-RvCAHS8
Plasmid#192467PurposeBacterial expression of RvCAHS8DepositorInsertCAHS8
UseTagsHis6ExpressionBacterialMutationPromoterT7Available sinceDec. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEThT-RvCAHS3
Plasmid#192466PurposeBacterial expression of RvCAHS3DepositorInsertCAHS3
UseTagsHis6ExpressionBacterialMutationPromoterT7Available sinceDec. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pX458-MMETn-del-gRNA2
Plasmid#185007Purposeexpression of gRNA and Cas9 from S. pyogenes with 2A-EGFPDepositorInsertMMETn-del-gRNA2
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJune 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pX458-IAP-del-gRNA1
Plasmid#185002Purposeexpression of gRNA and Cas9 from S. pyogenes with 2A-EGFPDepositorInsertIAP-del-gRNA1 (Akp3 Mouse)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJune 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pX458-MMERVK9C-del-gRNA1
Plasmid#185004Purposeexpression of gRNA and Cas9 from S. pyogenes with 2A-EGFPDepositorInsertMMERVK9C-del-gRNA1
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJune 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pX458-MMETn-del-gRNA1
Plasmid#185006Purposeexpression of gRNA and Cas9 from S. pyogenes with 2A-EGFPDepositorInsertMMETn-del-gRNA1
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJune 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pX458-MMERVK9C-del-gRNA2
Plasmid#185005Purposeexpression of gRNA and Cas9 from S. pyogenes with 2A-EGFPDepositorInsertMMERVK9C-del-gRNA2
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJune 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pX458-IAP-del-gRNA2
Plasmid#185003Purposeexpression of gRNA and Cas9 from S. pyogenes with 2A-EGFPDepositorInsertIAP-del-gRNA2 (Akp3 Mouse)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJune 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGreenII 0229/ProRUBY:GOXL6-Citrine:NOSt
Plasmid#175568PurposepSOUP helper plasmid needed for Agrobacterium-mediated plant transformation - Expression of GOXL genes tagged with Citrine under RUBY promoter for transgene complementation of ruby-6DepositorInsertGOXL6
UseTagsCitrineExpressionPlantMutationPromoterRUBYAvailable sinceJan. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGreenII 0229/ProRUBY:GOXL5-Citrine:NOSt
Plasmid#175567PurposepSOUP helper plasmid needed for Agrobacterium-mediated plant transformation - Expression of GOXL genes tagged with Citrine under RUBY promoter for transgene complementation of ruby-5DepositorInsertGOXL5
UseTagsCitrineExpressionPlantMutationPromoterRUBYAvailable sinceOct. 20, 2021AvailabilityAcademic Institutions and Nonprofits only