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pICSL11062
Plasmid#68256PurposeLevel 1 Golden Gate Cassette: sgRNA cassette targeting BolC.GA4a-2 in Brassica oleraceaDepositorInsertPromoter_AtU6-26 + sgRNA_BolC.GA4a-2
UseSynthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceAug. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHL391
Plasmid#62821PurposeLim Lab plasmid: AmpR + pLlacO-1::ompC::gfp::T1T2 terminator + PLtetO-1::RBS (st7) hfq::mCherry::T1 terminator + ColE1DepositorInsertsgfp
mCherry
UseSynthetic BiologyTagshfp leader, ompC leader, and st7 RBSExpressionBacterialMutationPromoterpLlacO-1 and pLtetO-1Available sinceAug. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHL518
Plasmid#62823PurposeLim Lab plasmid: AmpR + PLlacO-1::micC::mCherry::T1 terminator + PLtetO-1m9::OmpC::gfp::T1T2 terminator + ColE1DepositorInsertsmCherry
gfp
UseSynthetic BiologyTagsm9::ompC leader and micC leaderExpressionBacterialMutationPromoterpLlacO-1 and pLtetO-1Available sinceAug. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHL2021
Plasmid#62833PurposeLim Lab plasmid: AmpR + PLlacO-1::T710 hfq::T1 terminator + PLtetO-1m9::sodB::gfp::T1T2 terminator + ColE1DepositorInsertsT710 hfq leader
gfp
UseSynthetic BiologyTagsm9::sodB leaderExpressionBacterialMutationPromoterpLlacO-1 and pLtetO-1Available sinceAug. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHL2026
Plasmid#62834PurposeLim Lab plasmid: AmpR + PLlacO-1::T710 hfq::T1 terminator + PLtetO-1m9::glmS::gfp::T1T2 terminator + ColE1DepositorInsertsT710 hfq leader
gfp
UseSynthetic BiologyTagsm9::glmS leaderExpressionBacterialMutationPromoterpLlacO-1 and pLtetO-1Available sinceAug. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHL2027
Plasmid#62835PurposeLim Lab plasmid: AmpR + PLlacO-1::T710 hfq::T1terminator + PLtetO-1::glmS::gfp::T1T2 terminator + ColE1DepositorInsertsT710 hfq leader
gfp
UseSynthetic BiologyTagsglmS leaderExpressionBacterialMutationPromoterpLlacO-1 and pLtetO-1Available sinceAug. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHL2053
Plasmid#62836PurposeLim Lab plasmid: AmpR + PLlacO-1::T710::hfq::T1 termintor + PLtetO-1m9::ompC::gfp::T1T2 terminator + ColE1DepositorInsertsT710 hfq leader
gfp
UseSynthetic BiologyTagsm9::ompC leaderExpressionBacterialMutationPromoterpLlacO-1 and pLtetO-1Available sinceAug. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHL2073
Plasmid#62837PurposeLim Lab plasmid: AmpR + PconShortNoHind::glmZ::Asp terminator + PLlacO-1::T710 hfq::T1 terminator + PLtetO-1m9::glmS::gfp::T1T2 terminator + ColE1DepositorInsertsglmZ leader
T710 hfq leader
gfp
UseSynthetic BiologyTagsm9::glmSExpressionBacterialMutationPromoterpConShortNoHind, pLlacO-1, and pLtetO-1Available sinceAug. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHL2075
Plasmid#62838PurposeLim Lab plasmid: AmpR + PconShortNoHind::glmZ::Asp terminator + PLlacO-1::T710 hfq::T1 terminator + PLtetO-1::glmS::gfp::T1T2 terminator + ColE1DepositorInsertsglmZ leader
T710 hfq leader
gfp
UseSynthetic BiologyTagsglmSExpressionBacterialMutationPromoterpConShortNoHind, pLlacO-1, and pLtetO-1Available sinceAug. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHL2085
Plasmid#62840PurposeLim Lab plasmid: AmpR + PconShortNoHindM8::glmZ::Asp terminator + PLlacO-1::T710 hfq::T1 terminator + PLtetO-1::glmS::gfp::T1T2 terminator + ColE1DepositorInsertsm8::glmZ leader
T710 hfq leader
gfp
UseSynthetic BiologyTagsglmSExpressionBacterialMutationPromoterpConShortNoHind, pLlacO-1, and pLtetO-1Available sinceAug. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHL2117
Plasmid#62842PurposeLim Lab plasmid: AmpR + PconNoHindM8::ryhB::Asp terminator + PLtetO-1m9::sodB::gfp::T1T2 terminator + ColE1DepositorInsertsM8::ryhB leader
gfp
UseSynthetic BiologyTagsm9::sodB leaderExpressionBacterialMutationPromoterpConShortNoHind and pLtetO-1Available sinceAug. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHL2084
Plasmid#62839PurposeLim Lab plasmid: AmpR + PconShortNoHindM8::glmZ::Asp terminator + PLlacO-1::T710 hfq::T1 terminator + PLtetO-1m9::glmS::gfp::T1T2 terminator + ColE1DepositorInsertsm8::glmZ leader
T710 hfq leader
gfp
UseSynthetic BiologyTagsm9::glmSExpressionBacterialMutationPromoterpConShortNoHind, pLlacO-1, and pLtetO-1Available sinceMay 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
p3.2mar(T->G)-Luc
Plasmid#63697Purpose3.2 kb marine armor plate enhancer with a single T->G bp change driving Luciferase expressionDepositorInsert3.2 kb marine stickleback armor plate enhancer with a single T->G base pair change
UseTagsLuciferaseExpressionMammalianMutationPromoterTATA boxAvailable sinceMay 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
p3.2mar(T->G)-GFP
Plasmid#63695Purpose3.2 kb marine armor plate enhancer with a single T->G bp change driving GFP reporterDepositorInsert3.2 kb marine stickleback armor plate enhancer with a single T->G base pair change
UseTagsEGFPExpressionMammalianMutationT->G bp changePromoterhsp70Available sinceApril 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
pNB693
Plasmid#60145PurposeDisintegrator plasmid encoding MET17 promoter driven yellow beetle luciferase with nuclear localization signal (NLS) and ClpXP degron tag (ssrA).DepositorInsertYeLuc
UseTagsClpXP degron and Nuclear localization signal (NLS…ExpressionYeastMutationPromoterMET17prAvailable sinceDec. 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBacPak-FLAG-HIS-Sgf11
Plasmid#52287PurposeExpresses Drosophila Sgf11DepositorInsertSgf11 (Sgf11 Fly)
UseTagsHIS, FLAGExpressionInsectMutationPromoterAvailable sinceMay 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBacPak-HIS-FLAG-CG9866
Plasmid#52290PurposeExpresses Drosophila Ataxin-7DepositorInsertAtaxin-7 (CG9866 Fly)
UseTagsHIS FLAGExpressionInsectMutationPromoterAvailable sinceApril 24, 2014AvailabilityAcademic Institutions and Nonprofits only
