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Showing: 5161 - 5180 of 158205 results

  1. pBetaActin-FKBP-mScarlet-KIFC1motor

    Plasmid
    #191334
    Purpose
    Expresses the fluorescently labeled minus-end directed KIFC1 motor domain fused to the dimerization domain FKBP to recruit it to the plasma membrane by using the chemical dimerization system FKBP-FRB
    Depositor
    Frank Bradke
    Article
    Schelski et al Sci Adv. 2022 Nov 4;8(44):eabo2336. doi:
    Insert
    FKBP-mScarlet-KIFC1 (KIFC1 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    motor domain aa 125-673; N593K
    Promoter
    Available since
    Jan. 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    191334-plasmid-map-sequence-id-381755

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  6. pUC-dirTopo-AU1-aGFP

    Plasmid
    #61973
    Purpose
    A directional cloning vector of PCR-amplified cDNAs for mammalian expression
    Depositor
    Hiroshi Udo
    Inserts
    lacZ alpha
    EGFP
    Use
    Tags
    Expression
    Mammalian
    Mutation
    It contains silent mutations
    Promoter
    Available since
    Feb. 4, 2015
    Availability
    Academic Institutions and Nonprofits only
    61973-plasmid-map-sequence-id-101284

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  7. gCBEv2

    Plasmid
    #220617
    Purpose
    vector for encoding an engineered glycosylase-based cytosine (C) base editor (gCBEv2) driven by EF1a promoter, guide RNAs compatible with SpCas9 driven by hU6, mCherry driven by CBh promoter
    Depositor
    Huawei Tong
    Article
    Tong et al Nat Commun. 2024 Jun 8;15(1):4897. doi:
    Insert
    hUNG2-nSpCas9
    Use
    Tags
    Expression
    Mammalian
    Mutation
    hUNG2(del1-88;N213D,K184A,A214V), SpCas9(D10A)
    Promoter
    hU6, EF1a
    Available since
    June 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    220617-plasmid-map-sequence-id-436955

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  9. pCAS

    Plasmid
    #60847
    Purpose
    Expresses S. pyogenes Cas9 plus an HDV ribozyme-sgRNA for genome editing in yeast
    Depositor
    Jamie Cate
    Article
    Ryan et al Elife. 2014 Aug 19;3. doi: 10.7554/eLife
    Inserts
    S. pyogenese Cas9
    RNA pol III promoter (tRNA-Tyr)
    hepatitis delta virus ribozyme, genomic
    sgRNA
    Use
    CRISPR and Synthetic Biology
    Tags
    NLS/His8 and TTT 3' extension prior to sgRNA
    Expression
    Bacterial and Yeast
    Mutation
    L4 is UUCG tetraloop and guide targets LYP1 (CATA…
    Promoter
    Available since
    Jan. 13, 2015
    Availability
    Academic Institutions and Nonprofits only
    60847-plasmid-map-sequence-id-197511

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  17. pLenti-EF1a-Luciferase-IRES-Blast-WPRE

    Plasmid
    #108542
    Purpose
    Lentivirus vector expressing Luciferase with blasticidin resistance
    Depositor
    Javier Alcudia
    Inserts
    Luciferase
    Neomycin
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    EF1apha and PGK
    Available since
    April 19, 2018
    Availability
    Academic Institutions and Nonprofits only
    108542-plasmid-map-sequence-id-208671

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  19. p663-UBC-miniTurbo-V5-CDKL5_IDG-K

    Plasmid
    #170578
    Purpose
    Gateway destination clone of CDKL5 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics
    Depositor
    Ben Major
    Insert
    miniTurbo-V5-CDKL5 (CDKL5 Human)
    Use
    Lentiviral; Gateway destination
    Tags
    miniTurbo-V5
    Expression
    Mammalian
    Mutation
    E854G- please see depositor comments
    Promoter
    Ubiquitin
    Available since
    Sept. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    170578-plasmid-map-sequence-id-340993

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Showing: 5161 - 5180 of 158205 results