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Viral Prep#100839-AAV1PurposeReady-to-use AAV1 particles produced from pAAV.CAG.Flex.GCaMP6m.WPRE.SV40 (#100839). In addition to the viral particles, you will also receive purified pAAV.CAG.Flex.GCaMP6m.WPRE.SV40 plasmid DNA. CAG-driven, Cre-dependent GCaMP6m calcium sensor. These AAV preparations are suitable purity for injection into animals.DepositorPromoterCAGTagsNoneAvailable sinceMay 11, 2018AvailabilityAcademic Institutions and Nonprofits only
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pET21a-SerRS-His
Plasmid#124118PurposeBacterial expression plasmid for PURE system componentsDepositorInsertSeryl-tRNA ligase (serS Escherichia coli)
UseTags6x-HisExpressionBacterialMutationPromoterAvailable sinceApril 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
EGFP-Rab7A Q67L
Plasmid#28049DepositorInsertRab7A (RAB7A Human)
UseTags2xHA and EGFPExpressionMammalianMutationQ67LPromoterAvailable sinceMay 27, 2011AvailabilityAcademic Institutions and Nonprofits only -
pAAV_hSyn1_nLightG (AAV9)
Viral Prep#187179-AAV9PurposeReady-to-use AAV9 particles produced from pAAV_hSyn1_nLightG (#187179). In addition to the viral particles, you will also receive purified pAAV_hSyn1_nLightG plasmid DNA. Synapsin-driven expression of norepinephrine sensor nLightG. These AAV preparations are suitable purity for injection into animals.DepositorPromoterhuman Synapsin-1TagsNoneAvailable sinceJune 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
PBCAG-eGFP
Plasmid#40973DepositorInsertsPiggyBac transposon 3' terminal repeats
PiggyBac 5' terminal repeats
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGR618
Plasmid#213786PurposeIntegrates at the CAN1 locus the BB-Tv TP-DNAP1 variant and the Nourseothricin resistance marker. Includes an I-SceI transient expression cassetteDepositorInsertBB-Tv TP-DNAP1
UseSynthetic BiologyTagsExpressionBacterial and YeastMutationPromoterAvailable sinceMarch 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEvol-MjaYRS
Plasmid#153557PurposeAmber suppression for incorporating 3-aminotyrosine to proteins in E. coliDepositorInsertsMJaYRS (first copy)
MJaYRS (second copy)
amber suppression tRNA under proK promoter
UseTagsExpressionBacterialMutationPromoterglnS and pBADAvailable sinceJuly 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn Con/Fon hChR2(H134R)-EYFP (AAV5)
Viral Prep#55645-AAV5PurposeReady-to-use AAV5 particles produced from pAAV-hSyn Con/Fon hChR2(H134R)-EYFP (#55645). In addition to the viral particles, you will also receive purified pAAV-hSyn Con/Fon hChR2(H134R)-EYFP plasmid DNA. Synapsin-driven, Cre-dependent and Flp-dependent channelrhodopsin-EYFP expression for optogenetic activation. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagsEYFP (Cre- and Flp-dependent)Available sinceDec. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLPCX mito roGFP2-Orp1
Plasmid#64992PurposeMammalian expression of mitochondrial roGFP2-Orp1 (retroviral vector)DepositorInsertOrp1 (HYR1 Budding Yeast)
UseRetroviralTagsroGFP2ExpressionMammalianMutationmitochondrial targeting sequencePromoterAvailable sinceJune 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLAMP1-emiRFP670
Plasmid#136570PurposeLAMP1 (Lysosome Membrane) labeling with near-infrared fluorescent protein emiRFP670 (enhanced miRFP670)DepositorInsertLAMP1-emiRFP670
UseTagsemiRFP670ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
EF1a_mCherry_P2A_Hygro
Plasmid#135003PurposeLentiviral vector to express mCherry or to clone and express gene of interest, under control of EF1a promoter. Barcodes compatible with detection in single cell RNA-seq may be inserted at HpaI site.DepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceFeb. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDN-Sth1Cas9
Plasmid#221386PurposeCas9 under control of AHT inducible TetR on integrating plasmid (KanR) using L5 integrase and attP for site-specific integration into attBDepositorInsertSth1 Cas9
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJuly 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDN-Cherry-sgRNA
Plasmid#221387Purposeepisomal (HygR), with constitutive mCherry (Psmyc) and AHT-inducible sgRNA cloning site, including Golden Gate for multiple sgRNAsDepositorInsertnone, cloning vector for introduction of targeting sequences
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJuly 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
ER-GCaMP6-150
Plasmid#86918PurposeFluorescent reporter for ER calcium signalingDepositorInsertER-GCaMP6-150
UseLentiviralTagsExpressionMammalianMutationK78H, T302L, R303P, A317E, D324G, D360G, D380Y, T…PromoterCAMKIIAvailable sinceMarch 15, 2017AvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPR v2-dCas9
Plasmid#112233PurposeLentiviral plasmid for expression of gRNA and dCas9 in mammalian cells; derivative of lentiCRISPR v2DepositorInsertsdCas9
Porumycin resistance
UseCRISPR and LentiviralTagsFLAGExpressionMammalianMutationChanged: Aspartic acid 10 to Glycine, Histidine 8…PromoterEFS-NSAvailable sinceJune 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHAGE_puro
Plasmid#118692PurposeEmpty vector to express overexpression cDNADepositorTypeEmpty backboneUseLentiviralTagsExpressionMutationPromoterCMVAvailable sinceDec. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
Mouse Toronto KnockOut (mTKO) CRISPR Library - lentiCRISPRv2
Pooled Library#159392PurposeMouse lentiviral genome-wide CRISPR libraryDepositorExpressionMammalianSpeciesMus musculusUseCRISPR and LentiviralAvailable sinceNov. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCXLE-hMLN
Plasmid#27079PurposeNon-integrating (episomal) expression of human C-MYC, LIN28 and NANOGDepositorInsertC-MYC, LIN28, NANOG (MYC Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 6, 2011AvailabilityAcademic Institutions and Nonprofits only -
pMIEG3-JunDN
Plasmid#40350DepositorInsertJun dominant negative (JUN Human)
UseRetroviralTagsHA and IRES eGFPExpressionMammalianMutationfirst 122 amino acids deletedPromoterAvailable sinceSept. 28, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCAGIG
Plasmid#11159PurposeMammalian expression vector for cloning and expressing your gene on a bicistronic mRNA also encoding IRES-GFPDepositorHas ServiceCloning Grade DNAInsertIRES-EGFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 13, 2006AvailabilityAcademic Institutions and Nonprofits only