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Plasmid#140829PurposeAIMTOR BRET Biosensor containing a mutated non-phosphorylable ULK1 peptide to use as a control constuct in parrallel with AIMTOR T757DepositorInsertAIMTOR A757 (ULK1 Human)
UseTagsNES Nuclear export signalExpressionMammalianMutationThe insert comprises only a small sequence of hum…PromoterAvailable sinceJuly 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET-45b-mEGFP-HMGB1-WT
Plasmid#194543PurposeBacterial Expression of 6xHis mEGFP-HMGB1 WT Fusion ProteinDepositorInsertHMGB1 (HMGB1 Human)
UseTagsmEGFPExpressionBacterialMutationPromoterAvailable sinceFeb. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRK5-mEGFP-HMGB1-WT-Full-length
Plasmid#194548PurposeMammalian Expression of mEGFP-HMGB1 WT Fusion ProteinDepositorInsertHMGB1 (HMGB1 Human)
UseTagsmEGFPExpressionMammalianMutationPromoterAvailable sinceFeb. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pVQ CMV NanoV1-2a-EGFP ferritin
Plasmid#79649PurposeExpresses camelid anti-GFP nanobody fused to TRPV1 and GFP-ferritin chimera fusion proteinDepositorInsertsUseAdenoviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRK5-mEGFP-SOX2-WT
Plasmid#194580PurposeMammalian Expression of mEGFP- SOX2 WT Fusion ProteinDepositorInsertSOX2 (SOX2 Human)
UseTagsmEGFPExpressionMammalianMutationPromoterAvailable sinceFeb. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRK5-mEGFP-HMGB1-WT-IDR
Plasmid#194549PurposeMammalian Expression of mEGFP-HMGB1 WT IDR Fusion ProteinDepositorInsertHMGB1 (HMGB1 Human)
UseTagsmEGFPExpressionMammalianMutationPromoterAvailable sinceFeb. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pVQ CMV NanoV1Mutant-2a-EGFP ferritin
Plasmid#79650PurposeExpresses camelid anti-GFP nanobody fused to TRPV1 with mutant pore region and GFP-ferritin chimera fusion proteinDepositorInsertsUseAdenoviralTagsExpressionMammalianMutationisoleucine 679 changed to lysinePromoterCMVAvailable sinceSept. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRK5-mEGFP-FOXF1-WT
Plasmid#194568PurposeMammalian Expression of mEGFP-FOXF1 WT Fusion ProteinDepositorInsertFOXF1 (FOXF1 Human)
UseTagsmEGFPExpressionMammalianMutationPromoterAvailable sinceFeb. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRK5-mEGFP-HMGB3-WT
Plasmid#194564PurposeMammalian Expression of mEGFP-HMGB3 WT Fusion ProteinDepositorInsertHMGB3 (HMGB3 Human)
UseTagsmEGFPExpressionMammalianMutationPromoterAvailable sinceFeb. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRK5-mEGFP-FOXL2-WT
Plasmid#194584PurposeMammalian Expression of mEGFP-FOXL2 WT Fusion ProteinDepositorInsertFOXL2 (FOXL2 Human)
UseTagsmEGFPExpressionMammalianMutationPromoterAvailable sinceFeb. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRK5-mEGFP-FOXF1-MUT
Plasmid#194569PurposeMammalian Expression of mEGFP-FOXF1 Mutant (A231Rfs*61) Fusion ProteinDepositorInsertFOXF1 (FOXF1 Human)
UseTagsmEGFPExpressionMammalianMutationA231Rfs*61PromoterAvailable sinceFeb. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRK5-mEGFP-RUNX1-MUT
Plasmid#194575PurposeMammalian Expression of mEGFP-RUNX1 Mutant (G363Afs*229) Fusion ProteinDepositorInsertRUNX1 (RUNX1 Human)
UseTagsmEGFPExpressionMammalianMutationG363Afs*229PromoterAvailable sinceFeb. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRK5-mEGFP-HMGB3-MUT
Plasmid#194565PurposeMammalian Expression of mEGFP-HMGB3 Mutant (K161Ifs*55) Fusion ProteinDepositorInsertHMGB3 (HMGB3 Human)
UseTagsmEGFPExpressionMammalianMutationK161Ifs*55PromoterAvailable sinceFeb. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRK5-mEGFP-SOX2-MUT
Plasmid#194581PurposeMammalian Expression of mEGFP-SOX2 Mutant (M276Ifs*95) Fusion ProteinDepositorInsertSOX2 (SOX2 Human)
UseTagsmEGFPExpressionMammalianMutationM276Ifs*95PromoterAvailable sinceFeb. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRK5-mEGFP-CALR-MUT
Plasmid#194579PurposeMammalian Expression of mEGFP-CALR Mutant (D387Rfs*44) Fusion ProteinDepositorInsertCALR (CALR Human)
UseTagsmEGFPExpressionMammalianMutationD387Rfs*44PromoterAvailable sinceFeb. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRK5-mEGFP-HMGB1-delIDR
Plasmid#194554PurposeMammalian Expression of mEGFP-HMGB1 WT Fusion Protein, "del IDR" variantDepositorInsertHMGB1 (HMGB1 Human)
UseTagsmEGFPExpressionMammalianMutationtruncation by stop codon after Asn134PromoterAvailable sinceFeb. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRK5-mEGFP-SQSTM1-WT
Plasmid#194582PurposeMammalian Expression of mEGFP-SQSTM1 WT Fusion ProteinDepositorInsertSQSTM1 (SQSTM1 Human)
UseTagsmEGFPExpressionMammalianMutationPromoterAvailable sinceFeb. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRK5-mEGFP-FOXC1-MUT
Plasmid#194567PurposeMammalian Expression of mEGFP-FOXC1 Mutant (Q200Rfs*109) Fusion ProteinDepositorInsertFOXC1 (FOXC1 Human)
UseTagsmEGFPExpressionMammalianMutationQ200Rfs*109PromoterAvailable sinceFeb. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET-28a-mCherry-MED1-IDR
Plasmid#194545PurposeBacterial Expression of 6xHis mCherry-MED1-IDR Fusion ProteinDepositorInsertMED1 (MED1 Human)
UseTagsmCherryExpressionBacterialMutationPromoterAvailable sinceFeb. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
AIMTOR T757
Plasmid#140828PurposeAIMTOR T757 contains a cytoplasmic mTOR Activity Reporter derived from hu ULK1 protein boxed between BRET-compatible entities (Ypet and Nanoluciferase) to measure mTOR activity in living cellsDepositorInsertAIMTOR T757 (ULK1 Human)
UseTagsNES Nuclear export signalExpressionMammalianMutationThe insert comprises only a small sequence of hum…PromoterAvailable sinceJuly 10, 2020AvailabilityAcademic Institutions and Nonprofits only