Showing: 5121 - 5140 of 16077 results
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Plasmid#26185DepositorInsertMed21 aa1-132 for C-terminal tagging
UseSynthetic Biology; 2ab assembly vectorTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pRSF-Ivy-his-hLYZ
Plasmid#83920PurposeCo-expresses a hisx6 tagged copy of the Ivy protein and the wild type human lysozyme protein, both in the cytoplasmic compartment.DepositorInsertsIvy
lysozyme
UseTagsIvy protein has C-terminal hisx6 tagExpressionBacterialMutationIvy leader sequence deleted and replaced.PromoterT7 and t7AvailabilityAcademic Institutions and Nonprofits only -
pVH009
Plasmid#80399PurposeControl plasmid with no anti-scaffold feedback. Contains pBAD-CusR (response regulator) and pCusR-YFP-AAV.DepositorUseSynthetic BiologyTagsC-terminal LZx domain and N-terminal 3xFLAG tag, …ExpressionBacterialMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pET28a/Cas9-Cys
Plasmid#53261PurposeExpresses N-terminal His tag fused human codon-optimized Cas9 nuclease having C-terminal CysteineDepositorInsertCas9-Cys
UseTags6X His tag, HA epitope, and NLSExpressionBacterialMutationAdditional Cysteine is added to the C-terminal re…PromoterT7 PromoterAvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.1 (-) + FLAG-NLS-MS2-eGFP
Plasmid#86827PurposeExpresses a nuclear localized, FLAG-tagged MS2-GFP fusion protein in mammalian cells, used for purification or localization of tagged RNAsDepositorInsertFLAG-NLS-MS2-eGFP
UseTagsExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
6xHis-TEV-UBC-9
Plasmid#87055PurposeExpresses 6xHis-tagged ubc-9, with a TEV protease cleavage site between the tag and the ORFDepositorInsertUBC-9 (ubc-9 Nematode)
UseTags6xHis-TEVExpressionBacterialMutationPromoterT7AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-JNKsubDD-YPet-NES
Plasmid#84633PurposeEncodes C-terminal (substrate) fragment of bimolecular JNK activity reporter (bimJNKAR); cytosol targeted; use in conjunction with pcDNA3-Cerulean-FHA1-NESDepositorInsertJNKsubDD-YPet-NES
UseTags6xHis, Nuclear export signal (NES), T7 tag (gene …ExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
HOXA9 (murine) FLAG pET
Plasmid#8494DepositorInsertHOXA9 (Hoxa9 Mouse)
UseTagsFLAG and HisExpressionBacterialMutationFLAG epitope tag replaces T7 tag in the pET back…PromoterAvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 merlin 308-579
Plasmid#11626DepositorInsertmerlin (NF2 Human)
UseTagsFlagExpressionMammalianMutationCarboxy-terminal common domain of merlin.PromoterAvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 merlin 308-590
Plasmid#11627DepositorInsertmerlin (NF2 Human)
UseTagsFlagExpressionMammalianMutationCarboxy-terminal half of merlin isoform 2.PromoterAvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 merlin 308-595
Plasmid#11628DepositorInsertmerlin (NF2 Human)
UseTagsFlagExpressionMammalianMutationCarboxy-terminal half of merlin isoform 1.PromoterAvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-ExRai-CKAR
Plasmid#118409PurposecpGFP-based excitation-ratiometric biosensor for monitoring Protein Kinase C activity.DepositorInsertExRai-CKAR
UseTags6xHIS, T7 tag (gene 10 leader), and Xpress (TM) t…ExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-ExRai-AktAR
Plasmid#118462PurposecpGFP-based excitation-ratiometric biosensor for monitoring Akt/Protein Kinase B activity.DepositorInsertExRai-AktAR
UseTags6xHIS, T7 tag (gene 10 leader), and Xpress (TM) t…ExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-sapphireAKAR(T/A)
Plasmid#118465PurposeNegative-control mutant for sapphireAKAR biosensor.DepositorInsertsapphireAKAR(T/A)
UseTags6xHis, T7 tag (gene 10 leader), and Xpress (TM) t…ExpressionMammalianMutationContains Thr-to-Ala mutation in substrate sequenc…PromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-sapphireCKAR
Plasmid#118468Purposecp-T-sapphire-based single-color biosensor for monitoring Protein Kinase C activity.DepositorInsertsapphireCKAR
UseTags6xHIS, T7 tag (gene 10 leader), and Xpress (TM) t…ExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-sapphireCKAR(T/A)
Plasmid#118469PurposeNegative-control mutant for sapphireCKAR biosensor.DepositorInsertsapphireCKAR(T/A)
UseTags6xHIS, T7 tag (gene 10 leader), and Xpress (TM) t…ExpressionMammalianMutationContains Thr-to-Ala mutation in substrate sequenc…PromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-blueAKAR(T/A)
Plasmid#118471PurposeNegative-control mutant for blueAKAR biosensor.DepositorInsertblueAKAR(T/A)
UseTags6xHis, T7 tag (gene 10 leader), and Xpress (TM) t…ExpressionMammalianMutationContains Thr-to-Ala mutation in substrate sequenc…PromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-blueCKAR
Plasmid#118472PurposecpBFP-based single-color biosensor for monitoring Protein Kinase C activity.DepositorInsertblueCKAR
UseTags6xHIS, T7 tag (gene 10 leader), and Xpress (TM) t…ExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-blueCKAR(T/A)
Plasmid#118473PurposeNegative-control mutant for blueCKAR biosensor.DepositorInsertblueCKAR(T/A)
UseTags6xHIS, T7 tag (gene 10 leader), and Xpress (TM) t…ExpressionMammalianMutationContains Thr-to-Ala mutation in substrate sequenc…PromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-RAB-EKARev
Plasmid#118474PurposeddRFP-based single-color biosensor for monitoring ERK activity.DepositorInsertRAB-EKARev
UseTags6xHIS, T7 tag (gene 10 leader), and Xpress (TM) t…ExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only
Showing: 5121 - 5140 of 16077 results