Addgene

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pcDNA 3.2/V5-DEST CDC42EP1 S192A

Plasmid

#69820

Purpose

Expresses CDC42EP1-V5 in mammalian cells

Depositor

Anne Brunet

Article

Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

Insert

CDC42 effector protein (CDC42EP1 Human)

Use

Tags

V5

Expression

Mammalian

Mutation

S192A

Promoter

CMV

Available since

April 22, 2016

Availability

Academic Institutions and Nonprofits only

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pet-ECHB(35-475)

Plasmid

#96890

Purpose

expresses His-tagged mouse ECHB (35-475) in E.coli cells

Depositor

Hening Lin

Article

Sadhukhan et al Proc Natl Acad Sci U S A. 2016 Apr 19;11

Insert

Trifunctional enzyme subunit beta, mitochondrial (Hadhb Mouse)

Use

Tags

His6 tag

Expression

Bacterial

Mutation

deleted amino acid 1-34

Promoter

T7

Available since

Dec. 3, 2020

Availability

Academic Institutions and Nonprofits only

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PRKAA2 gRNA (BRDN0001146900)

Plasmid

#76635

Purpose

3rd generation lentiviral gRNA plasmid targeting human PRKAA2

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PRKAA2 (PRKAA2 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

June 27, 2016

Availability

Academic Institutions and Nonprofits only

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pitx2_R (OZ534)

Plasmid

#27211

Depositor

Keith Joung

Article

Maeder et al Mol Cell. 2008 Jul 25. 31(2):294-301.

Insert

Zinc finger array targeting pitx2 (pitx2 Zebrafish)

Use

Zebrafish targeting

Tags

Expression

Mutation

Promoter

Available since

Feb. 25, 2011

Availability

Academic Institutions and Nonprofits only
pitx2_L (OZ533)

Plasmid

#27210

Depositor

Keith Joung

Article

Maeder et al Mol Cell. 2008 Jul 25. 31(2):294-301.

Insert

Zinc finger array targeting pitx2 (pitx2 Zebrafish)

Use

Zebrafish targeting

Tags

Expression

Mutation

Promoter

Available since

Jan. 25, 2011

Availability

Academic Institutions and Nonprofits only
MAPK15 gRNA (BRDN0001145787)

Plasmid

#75618

Purpose

3rd generation lentiviral gRNA plasmid targeting human MAPK15

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

MAPK15 (MAPK15 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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MAPK15 gRNA (BRDN0001148491)

Plasmid

#75619

Purpose

3rd generation lentiviral gRNA plasmid targeting human MAPK15

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

MAPK15 (MAPK15 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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MAPK15 gRNA (BRDN0001162376)

Plasmid

#75620

Purpose

3rd generation lentiviral gRNA plasmid targeting human MAPK15

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

MAPK15 (MAPK15 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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MAPK15 gRNA (BRDN0001162374)

Plasmid

#75621

Purpose

3rd generation lentiviral gRNA plasmid targeting human MAPK15

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

MAPK15 (MAPK15 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pDONR223_RAB31_WT_V5

Plasmid

#82993

Purpose

Gateway Donor vector containing RAB31, used as an over expression control for gene expression analysis. Part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, David Root, Matthew Meyerson

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

RAB31 (RAB31 Human)

Use

Gateway entry vector

Tags

Expression

Mutation

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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DCLK2 gRNA (BRDN0001162199)

Plasmid

#75701

Purpose

3rd generation lentiviral gRNA plasmid targeting human DCLK2

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

DCLK2 (DCLK2 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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PRKAA2 gRNA (BRDN0001147732)

Plasmid

#76636

Purpose

3rd generation lentiviral gRNA plasmid targeting human PRKAA2

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PRKAA2 (PRKAA2 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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PRKAA2 gRNA (BRDN0001147124)

Plasmid

#76637

Purpose

3rd generation lentiviral gRNA plasmid targeting human PRKAA2

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PRKAA2 (PRKAA2 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pLenti-multi-CRISPR-sgRipk3_#2-puro

Plasmid

#231982

Purpose

Knockout mouse Ripk3

Depositor

Brian Ruffell

Article

Hanggi et al Cancer Cell. 2024 Nov 13:S1535-6108(24)0

Insert

sgRNA with Cas9 with puromycin resistance (Ripk3 Mouse)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Feb. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pLenti-multi-CRISPR-sgRipk3_#1-puro

Plasmid

#231981

Purpose

Knockout mouse Ripk3

Depositor

Brian Ruffell

Article

Hanggi et al Cancer Cell. 2024 Nov 13:S1535-6108(24)0

Insert

sgRNA with Cas9 with puromycin resistance (Ripk3 Mouse)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Feb. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pLVX-DmrB-DmrB-Ripk3-2A-mCherry-puro

Plasmid

#231972

Purpose

Tet inducible expression of DmrB-Ripk3 with mCherry to induce Ripk3 necroptosis

Depositor

Brian Ruffell

Article

Hanggi et al Cancer Cell. 2024 Nov 13:S1535-6108(24)0

Insert

Ripk3-deltaC dimerizing construct with bi-cistronic mCherry (Ripk3 Mouse)

Use

Lentiviral

Tags

Expression

Mutation

WT

Promoter

Available since

Feb. 5, 2025

Availability

Academic Institutions and Nonprofits only

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pCS2-MYC-GFP-IRES-mCherry

Plasmid

#231232

Purpose

MYC stability reporter construct (expressing c-myc 2) for transient expression in mammalian cells. Stability of GFP-fusion protein can be assed by flow cytometry by normalizing to mCherry expression.

Depositor

Michael Rape

Article

Mark et al Cell. 2023 Aug 3;186(16):3460-3475.e23.

Insert

MYC (MYC Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 21, 2025

Availability

Academic Institutions and Nonprofits only

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pPBpuro-miRFP703-eDHFR(69K6)-cRaf

Plasmid

#209919

Purpose

Mammalian expression of cRaf fused to miRFP703-eDHFR(69K6)

Depositor

Michiyuki Matsuda

Article

Deguchi et al Cell Rep. 2024 Nov 14;43(11):114986. doi

Insert

miRFP703-eDHFR(69K6)-cRaf (RAF1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pPBbleo-miRFP703-eDHFR(69K6)-cRaf

Plasmid

#209920

Purpose

Mammalian expression of cRaf fused to miRFP703-eDHFR(69K6)

Depositor

Michiyuki Matsuda

Article

Deguchi et al Cell Rep. 2024 Nov 14;43(11):114986. doi

Insert

miRFP703-eDHFR(69K6)-cRaf (RAF1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pPBbsr2-miRFP703-eDHFR(69K6)-cRaf

Plasmid

#209921

Purpose

Mammalian expression of cRaf fused to miRFP703-eDHFR(69K6)

Depositor

Michiyuki Matsuda

Article

Deguchi et al Cell Rep. 2024 Nov 14;43(11):114986. doi

Insert

miRFP703-eDHFR(69K6)-cRaf (RAF1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 18, 2024

Availability

Academic Institutions and Nonprofits only

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