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Plasmid#105137PurposeYeast gene targetingDepositorInsertkanMX6-P3nmt1-mEGFP
UseTags3nmt1-mEGFPExpressionBacterial and YeastMutationA206KPromoter3nmt1Available sinceFeb. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
Efg1-ΔDBD-Y115S/Y132S/Y139S
Plasmid#216408PurposeExpresses 6xHis-tagged N- and C-terminal prion-like domains of Efg1 with Y115/Y132/Y139 to serine mutationsDepositorInsertEfg1-ΔDBD-Y115S/Y132S/Y139S
UseTags6xHis-tagsExpressionBacterialMutationchanged Tyrosine 115, 132 and 139 to Serine, and …PromoterAvailable sinceJuly 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
Efg1-ΔDBD-A51P/A56P/Q443P
Plasmid#216402PurposeExpresses 6xHis-tagged N- and C-terminal prion-like domains of Efg1 with A51/A56/Q443 to proline mutationsDepositorInsertEfg1-ΔDBD-A51P/A56P/Q443P
UseTags6xHis-tagsExpressionBacterialMutationchanged Alanine 51, 56 and Glutamine 443 to Proli…PromoterAvailable sinceApril 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
Efg1-ΔDBD-A51P/A56P/Q70P/Q443P
Plasmid#216403PurposeExpresses 6xHis-tagged N- and C-terminal prion-like domains of Efg1 with A51/A56/Q70P/Q443 to proline mutationsDepositorInsertEfg1-ΔDBD-A51P/A56P/Q70P/Q443P
UseTags6xHis-tagsExpressionBacterialMutationchanged Alanine 51, 56 and Glutamine 70, 443 to P…PromoterAvailable sinceApril 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
Efg1-ΔDBD-Y4S/Y8S/Y16S
Plasmid#216405PurposeExpresses 6xHis-tagged N- and C-terminal prion-like domains of Efg1 with Y4/Y8/Y16 to serine mutationsDepositorInsertEfg1-ΔDBD-Y4S/Y8S/Y16S
UseTags6xHis-tagsExpressionBacterialMutationchanged Tyrosine 4, 8 and 16 to Serine, and delet…PromoterAvailable sinceApril 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
Efg1-ΔDBD-Y64S/Y66S/Y69S
Plasmid#216406PurposeExpresses 6xHis-tagged N- and C-terminal prion-like domains of Efg1 with Y64/Y66/Y69 to serine mutationsDepositorInsertEfg1-ΔDBD-Y64S/Y66S/Y69S
UseTags6xHis-tagsExpressionBacterialMutationchanged Tyrosine 64, 66 and 69 to Serine, and del…PromoterAvailable sinceApril 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
Efg1-ΔDBD-Y101S/Y104S/Y106S
Plasmid#216407PurposeExpresses 6xHis-tagged N- and C-terminal prion-like domains of Efg1 with Y101/Y104/Y106 to serine mutationsDepositorInsertEfg1-ΔDBD-Y101S/Y104S/Y106S
UseTags6xHis-tagsExpressionBacterialMutationchanged Tyrosine 101, 104 and 106 to Serine, and …PromoterAvailable sinceApril 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
Efg1-ΔDBD-Y401S/Y404S/Y409S
Plasmid#216409PurposeExpresses 6xHis-tagged N- and C-terminal prion-like domains of Efg1 with Y401/Y404/Y409 to serine mutationsDepositorInsertEfg1-ΔDBD-Y401S/Y404S/Y409S
UseTags6xHis-tagsExpressionBacterialMutationchanged Tyrosine 401, 404 and 409 to Serine, and …PromoterAvailable sinceApril 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
Efg1-ΔDBD-Y450S/Y452S/Y456S
Plasmid#216410PurposeExpresses 6xHis-tagged N- and C-terminal prion-like domains of Efg1 with Y450/Y452/Y456 to serine mutationsDepositorInsertEfg1-ΔDBD-Y450S/Y452S/Y456S
UseTags6xHis-tagsExpressionBacterialMutationchanged Tyrosine 450, 452 and 456 to Serine, and …PromoterAvailable sinceApril 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
Efg1-ΔDBD-Y478S/Y482S/Y484S
Plasmid#216411PurposeExpresses 6xHis-tagged N- and C-terminal prion-like domains of Efg1 with Y478/Y482/Y484 to serine mutationsDepositorInsertEfg1-ΔDBD-Y478S/Y482S/Y484S
UseTags6xHis-tagsExpressionBacterialMutationchanged Tyrosine 478, 482 and 484 to Serine, and …PromoterAvailable sinceApril 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
12URA-B-ScTop2∆CTD
Plasmid#214924PurposeYeast expression vector 6xHisTag-TEV at N terminus to express S cerevisiae topoisomerase II (1–1177)DepositorInserttopoisomerase II
UseTags6x His-TEVExpressionYeastMutationDeleted amino acids 1178-1428PromoterGal 1/10Available sinceMarch 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYea5.1_Notch1-L24_sfGFP
Plasmid#207865PurposeYeast 2µ vector for expression of Suc2SP-Notch1-L24_100-sfGFP-GAL4DepositorInsertSuc2SP-Notch1-L24_100-sfGFP-GAL4 fusion protein
UseTagsStrep-TagIIExpressionBacterial and YeastMutationPromoterAvailable sinceDec. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pYea5.1_C99_sfGFP
Plasmid#207802PurposeYeast 2µ vector for expression of Suc2SP-C99-sfGFP-GAL4DepositorInsertSuc2SP-C100-sfGFP-GAL4 fusion protein
UseTagsStrep-TagIIExpressionBacterial and YeastMutationPromoterAvailable sinceDec. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pYea5.1_Notch3_sfGFP
Plasmid#207864PurposeYeast 2µ vector for expression of Suc2SP-Notch3_100-sfGFP-GAL4DepositorInsertSuc2SP-Notch3_100-sfGFP-GAL4 fusion protein
UseTagsStrep-TagIIExpressionBacterial and YeastMutationPromoterAvailable sinceDec. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pYea5.1_Notch1_sfGFP
Plasmid#207801PurposeYeast 2µ vector for expression of Suc2SP-Notch1_100-sfGFP-GAL4DepositorInsertSuc2SP-Notch1_100-sfGFP-GAL4 fusion protein
UseTagsStrep-TagIIExpressionBacterial and YeastMutationPromoterTEFAvailable sinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
Dhh1 25-425-EGFP
Plasmid#201430PurposeBacterial protein expression of Dhh1 25-425DepositorInsertDhh1 (DHH1 Budding Yeast)
UseTagsEGFP, TEV, 6xHis and MBP, TEVExpressionBacterialMutationaa 25-425 onlyPromotertacAvailable sinceJune 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
Dcp2 301-970-EGFP
Plasmid#201429PurposeBacterial protein expression of Dcp2 301-970DepositorInsertDcp2 (DCP2 Budding Yeast)
UseTagsEGFP, TEV, 6xHis and MBP, TEVExpressionBacterialMutationaa 301-970 onlyPromotertacAvailable sinceJune 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
Dhh1 426-506-EGFP
Plasmid#201431PurposeBacterial protein expression of Dhh1 426-506DepositorInsertDhh1 (DHH1 Budding Yeast)
UseTagsEGFP, TEV, 6xHis and MBP, TEVExpressionBacterialMutationaa 426-506 onlyPromotertacAvailable sinceMay 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
Dcp1 D82-129-EGFP
Plasmid#201426PurposeBacterial protein expression of Dcp1 del82-129DepositorInsertDcp1 (DCP1 Budding Yeast)
UseTagsEGFP, TEV, 6xHis and MBP, TEVExpressionBacterialMutationdeletion of aa 82-129PromotertacAvailable sinceMay 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
Edc3 1-66-EGFP
Plasmid#201432PurposeBacterial protein expression of Edc3 1-66DepositorInsertEdc3 (EDC3 Budding Yeast)
UseTagsEGFP, TEV, 6xHis and MBP, TEVExpressionBacterialMutationaa 1-66 onlyPromotertacAvailable sinceMay 26, 2023AvailabilityAcademic Institutions and Nonprofits only