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Plasmid#141058PurposeMutisite Gateway mediated vector constructionDepositorInsertNeomycin resistance gene
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pPB-DEST-R5R4-pPuroTK
Plasmid#141060PurposeMutisite Gateway mediated vector constructionDepositorInsertPuroTK
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLz75_CB_operon_CsoS2_N3-N4
Plasmid#140857PurposeSame as pLz37 but with CsoS2 truncated such that NTD repeats N1 and N2 are removedDepositorInsertCbbL, CbbS, CsoS2 (delN1-N2), CsoSCA, CsoS4A, CsoS4B, CsoS1C, CsoS1A, CsoS1B, CsoS1D
UseTagsExpressionBacterialMutationdeleted amino acids 1-109 of CsoS2PromoterAvailable sinceJune 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLz76_CB_operon_CsoS2_N4
Plasmid#140858PurposeSame as pLz37 but with CsoS2 truncated such that NTD repeats N1, N2, and N3 are removedDepositorInsertCbbL, CbbS, CsoS2 (delN1-N3), CsoSCA, CsoS4A, CsoS4B, CsoS1C, CsoS1A, CsoS1B, CsoS1D
UseTagsExpressionBacterialMutationdeleted amino acids 1-189 of CsoS2PromoterAvailable sinceJune 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFd022.R2
Plasmid#137967PurposeExpresses Prochlorococcus phage P-SSM2 phage FdDepositorInsertProchlorococcus phage P-SSM2 ferredoxin
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJune 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFd029.R2.RFP
Plasmid#137972PurposeExpresses Prochlorococcus marinus NATL1A Fd 1 fused to mRFPDepositorInsertProchlorococcus marinus NATL1A ferredoxin 1 fused to monomeric RFP
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJune 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFd030.R2.RFP
Plasmid#137973PurposeExpresses Prochlorococcus marinus NATL2A Fd 1 fused to mRFPDepositorInsertProchlorococcus marinus NATL2A ferredoxin 1 fused to monomeric RFP
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJune 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFd031.RFP
Plasmid#137974PurposeExpresses Prochlorococcus marinus MIT9211 Fd 1 fused to mRFPDepositorInsertProchlorococcus marinus MIT9211 ferredoxin 1 fused to monomeric RFP
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJune 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4046858
Plasmid#145658PurposeBacterial Expression plasmid for SARS-CoV-2 2'-O-ribose methyltransferaseDepositorInsertSARS-CoV-2 2'-O-ribose methyltransferase (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable TEV;HAExpressionBacterialMutationEscherichia coli recode 1PromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available sinceApril 29, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGBW-m4046730
Plasmid#145659PurposeBacterial Expression plasmid for SARS-CoV-2 E (envelope)DepositorInsertSARS-CoV-2 E (envelope) (E Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable TEV;HAExpressionBacterialMutationEscherichia coli recode 1PromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available sinceApril 29, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGBW-m4046823
Plasmid#145634PurposeBacterial Expression plasmid for SARS-CoV-2 2'-O-ribose methyltransferaseDepositorInsertSARS-CoV-2 2'-O-ribose methyltransferase (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable TEV;MycExpressionBacterialMutationEscherichia coli recode 1PromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available sinceApril 29, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGBW-m4046816
Plasmid#145622PurposeBacterial Expression plasmid for SARS-CoV-2 2'-O-ribose methyltransferaseDepositorInsertSARS-CoV-2 2'-O-ribose methyltransferase (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable TEV;FLAG_2ExpressionBacterialMutationEscherichia coli recode 1PromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available sinceApril 29, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGBW-m4046791
Plasmid#145586PurposeBacterial Expression plasmid for SARS-CoV-2 2'-O-ribose methyltransferaseDepositorInsertSARS-CoV-2 2'-O-ribose methyltransferase (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable TEV;StrepIIExpressionBacterialMutationEscherichia coli recode 1PromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available sinceApril 29, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGBW-m4046774
Plasmid#145574PurposeBacterial Expression plasmid for SARS-CoV-2 RNA-dependent RNA polymeraseDepositorInsertSARS-CoV-2 RNA-dependent RNA polymerase (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable TEV;FLAG_2ExpressionBacterialMutationEscherichia coli recode 1PromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available sinceApril 29, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGBW-m4046846
Plasmid#145575PurposeBacterial Expression plasmid for SARS-CoV-2 RNA-dependent RNA polymeraseDepositorInsertSARS-CoV-2 RNA-dependent RNA polymerase (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable TEV;HAExpressionBacterialMutationEscherichia coli recode 1PromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available sinceApril 29, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
MYMH 351
Plasmid#138058PurposeEncodes Ribosome Binding Site (RBS) variant using GFP as a reporter geneDepositorInsertpSEVA-331Bb-10AG 11AC-GFP
UseTagsExpressionBacterialMutationAAA GAG GAG GCAPromoterAvailable sinceApril 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
MYMH 336
Plasmid#138057PurposeEncodes Ribosome Binding Site (RBS) variant using GFP as a reporter geneDepositorInsertpSEVA-331Bb-1AG 2AC 11AC-GFP
UseTagsExpressionBacterialMutationGCA GAG GAG ACAPromoterAvailable sinceApril 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
MYMH 333
Plasmid#138056PurposeEncodes Ribosome Binding Site (RBS) variant using GFP as a reporter geneDepositorInsertpSEVA-331Bb-1AG 11AC-GFP
UseTagsExpressionBacterialMutationGAA GAG GAG ACAPromoterAvailable sinceApril 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
MYMH 330
Plasmid#138055PurposeEncodes Ribosome Binding Site (RBS) variant using GFP as a reporter geneDepositorInsertpSEVA-331Bb- 2AC 11AC-GFP
UseTagsExpressionBacterialMutationACA GAG GAG ACAPromoterAvailable sinceApril 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
MYMH 327
Plasmid#138054PurposeEncodes Ribosome Binding Site (RBS) variant using GFP as a reporter geneDepositorInsertpSEVA-331Bb-1AG 2AC-GFP
UseTagsExpressionBacterialMutationGCA GAG GAG AAAPromoterAvailable sinceApril 10, 2020AvailabilityAcademic Institutions and Nonprofits only