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pDEST-DHFR F[3]-C (LEU2)
Plasmid#177796PurposeGateway destination vector to insert genes of interest having a C-terminal DHFR F[3] fusion for DHFR-PCA/BFG-PCA assays.DepositorTypeEmpty backboneUseTagsDHFR F[3]ExpressionYeastMutationPromoterADH1Available sinceJan. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET-45b-NFYC-IDR-mEGFP
Plasmid#185015PurposeBacterial Expression of 6xHis NFYC IDR Sirius-GFP Fusion ProteinDepositorInsertNFYC IDR (Nfyc Mouse)
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
px335-sgRNA-EF(Cas9wt)-Rif1
Plasmid#122306PurposeExpresses sgRNA targeting mouse Rif1 and Cas9 in mammalian cellsDepositorInsertsgRNA for mouse Rif1 (Rif1 Synthetic)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
T7T3-PacI-elfn1_459to1199
Plasmid#87671PurposeIn situ probe for mouse Elfn1DepositorInsertElfn1 (Elfn1 Mouse)
UseTagsExpressionBacterialMutationpartial sequence, (459 to 1199bp off NM_175522.3)PromoterT7/T3Available sinceApril 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
shGLDC-DOX
Plasmid#72883Purposedox inducible knockdownDepositorInsertRNAi hairpin against GLDC
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSAM2_mCherry_Tbx5
Plasmid#72688PurposeDoxycyline regulated expression of Tbx5 in mammalian cellsDepositorInsertTbx 5 (Tbx5 Mouse)
UseLentiviralTagsExpressionMammalianMutationPromoterCMV-DoxycyclineAvailable sinceMarch 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSOMA-T679A-NES
Plasmid#118940PurposeUsed to express the T679A mutant form of the SOMA FRET sensor with a nuclear exclusion signal in Arabidopsis thalianaDepositorInsertSOMA Sensor for Arabidopsis MAPK activity with T679A Mutation and Nuclear Exclusion Signal
UseTagsExpressionPlantMutationPromoterAvailable sinceFeb. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
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pAAV-EF1a-NES-tdTomato-LINuS-WPRE
Plasmid#159894PurposeAAV expression of tdTomato fused to LINuS, a light-inducible nuclear localization signalDepositorInsertNES-tdTomato-LINuS
UseAAVTagsExpressionMammalianMutationPromoterEF1α promoterAvailable sinceSept. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
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pCDNA3/flag-pBirA(1-G78)-FRB
Plasmid#154447Purposeexpresses pBirA(1-G78)-FRB/biochemical assayDepositorInsertflag-pBirA(1-G78)-FRB
UseTagsExpressionMammalianMutationPromoterT7 promoterAvailable sinceAug. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSMART-ala10
Plasmid#87135PurposeNADPH dependent Alanine Dehydrogenase, Low Phosphate Dependent E. coli ExpressionDepositorUseTagsExpressionBacterialMutationD196A, L197RPromoterwaaHp from E. coli as part of operon with ald* an…Available sinceMarch 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
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pT2-7xTcf-NLS-YNL-CP
Plasmid#65714PurposeTol2-based Wnt signal reporter plasmid (expresses NLS-YNL-CP)DepositorInsert7xTcf, minCMV, 3xNLS, YNL, hCL1-PEST
UseLuciferaseTagsExpressionMutationPromoterAvailable sinceJan. 26, 2016AvailabilityAcademic Institutions and Nonprofits only
