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Plasmid#121394PurposeFor Yeast-two-hybrid analysisDepositorInsertATG17
UseTagsExpressionYeastMutationPromoterADH1 promoterAvailable sinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pNC1137
Plasmid#41563DepositorInsertUra3-FUS1-UBIMdkGFP*-SpHis5-Tim9
UseTagsExpressionMutationGFP mutations: Phe 64 to Leu, S65 to Thr, V163 t…PromoterAvailable sinceApril 2, 2013AvailabilityAcademic Institutions and Nonprofits only -
pJT10R
Plasmid#98307PurposeOverexpressing yeast farnesyl pyrophosphate synthase Erg20p mutant N127W and Actinidia chinensis nerolidol/linalool synhtase AcNES1 under the control of GAL1/GAL2 promoterDepositorInsertTRPL3AcNES1>TRPL41B
UseTagsExpressionYeastMutationERG20 N127WPromoterAvailable sinceFeb. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
p3E-ATG18
Plasmid#109556PurposeMultisite gateway vector for 3' tagging with yeast ATG18 (PLEKHA3). Binds PtdIns(3,5P)2DepositorInsertyeast ATG18 (PLEKHA3)
UseZebrafish plasmidsTagsExpressionMutationPromoterAvailable sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
BB2_EF
Plasmid#98538Purposeempty BB2 for transcription unit cloningDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pWZ414-F55
Plasmid#23079DepositorInsertyeast Histone H3-2 and Histone H4-2
UseTagsExpressionYeastMutationHistone H3 changed Lysine 9 to Arginine, H3 K9R …PromoterAvailable sinceMarch 3, 2010AvailabilityAcademic Institutions and Nonprofits only -
pCTcon2-5INS_EV3
Plasmid#102293PurposeYeast Surface Display of 5Ins_16_EV3DepositorInsertEvolved Insulin binding scFV
UseTags3x(G4S) Linker, G3S Linker, HA, and MycExpressionYeastMutationPromoterGAL1Available sinceNov. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJT2
Plasmid#98306PurposeOverexpressing yeast farnesyl pyrophosphate synthase Erg20p mutant N127W and Actinidia chinensis nerolidol/linalool synhtase AcNES1 under the control of TEF1/TEF2 promoterDepositorInsertPTEF2>ScERG20N127W>TRPL3>PTEF1>AcNES1>TRPL41B
UseTagsExpressionYeastMutationERG20 N127WPromoterAvailable sinceFeb. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJT1
Plasmid#98299PurposeOverexpressing yeast farnesyl pyrophosphate synthase Erg20p and Actinidia chinensis nerolidol/linalool synhtase AcNES1 under the control of TEF1/TEF2 promoterDepositorInsertPTEF2-ERG20-TRPL3-PTEF1-AcNES1-TRPL41B
UseTagsExpressionYeastMutationPromoterAvailable sinceAug. 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRS426-GFP-Num1-PH
Plasmid#71374PurposeFor analysis of PH domain localization in yeastDepositorInsertNum1-PH
UseTagsEGFPExpressionYeastMutationPleckstrin homology (PH) domain; aa2563-2692PromoterAvailable sinceFeb. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDZ305 GTL1 GAL1p 24xPP7 V3 <HIS>
Plasmid#72240PurposeHistidine selection; Used to insert a GAL1 promoter and 24 repetitive PP7 version 3 RNA stem-loops before GLT1's start codon (5' tagging)DepositorInsertGLT1 GAL1p 24xPP7 V3
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLLX8
Plasmid#79838PurposeContains yeast counter selectable marker, CYH2, and ampicillin resistance cassette, used for making a DNA capture vectorDepositorTypeEmpty backboneUseContains yeast counter selection gene, cyh2 and b…TagsExpressionMutationPromoterAvailable sinceSept. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
CMYA5/pACT2
Plasmid#97208PurposeCMYA5 (aa 4039 – 4069) in a GAL4 AD vector, used for yeast-two hybridDepositorInsertCMYA5 (CMYA5 Human)
UseTagsExpressionYeastMutationPromoterAvailable sinceAug. 21, 2017AvailabilityIndustry, Academic Institutions, and Nonprofits -
pFA6a-kanMX6-P41nmt1-mEGFP
Plasmid#105140PurposeYeast gene targetingDepositorInsertkanMX6-P41nmt1-mEGFP
UseTags41nmt1-mEGFPExpressionBacterial and YeastMutationA206KPromoter41nmt1Available sinceFeb. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRS406 ATG1
Plasmid#166846PurposeExpresses Atg1 with ATG1 3'UTR in yeasts.DepositorInsertATG1 (ATG1 Budding Yeast)
UseTagsExpressionYeastMutationPromoterATG1 PromoterAvailable sinceApril 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
A102 ΔAmpH Δ2x+ve
Plasmid#129484PurposeExpression of Glo3 ΔAmpH lacking the COPI-binding motif in E.ColiDepositorInsertADP-ribosylation factor GTPase-activating protein (GLO3 Budding Yeast)
UseTagsHis and MBPExpressionBacterialMutationPromoterAvailable sinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
A102-GAP-BoCCS-GRM
Plasmid#123286PurposeExpression of Glo3 ΔAmpH (1-459) in E.ColiDepositorInsertADP-ribosylation factor GTPase-activating protein (GLO3 Budding Yeast)
UseTagsHis and MBPExpressionBacterialMutationPromoterT5Available sinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJT23_GalL_BE-PAPAP
Plasmid#143486PurposeExpresses BE-PAPAP in yeast cellsDepositorInsertBE-PAPAP
UseCRISPRTagsExpressionYeastMutationspCas9(D10A)PromoterGalLAvailable sinceJuly 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRS416-PHO5-GFP-2xOsh2 PH domain EE mutation
Plasmid#58830PurposeExpresses GFP-Tagged and mutated Osh2 PH domain in yeastDepositorInsert2 x Osh2 PH domain (OSH2 Budding Yeast)
UseTagsGFP and MycExpressionYeastMutationchanged Lysine 307 to Glutamate and Arginine 309 …PromoterPHO5Available sinceSept. 30, 2014AvailabilityAcademic Institutions and Nonprofits only -
pRS406 ATG9 stopGFP
Plasmid#114669PurposeExpresses yeast ATG9 under the endogenous promoter with a stop codon introduced before GFP C-terminal tagDepositorInsertATG9
UseTagsGFPExpressionYeastMutationChanged the three linker base pairs after the las…PromoterATG9Available sinceSept. 14, 2018AvailabilityAcademic Institutions and Nonprofits only