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Plasmid#56084PurposeLocalization: Nucleoli, Excitation: 588, Emission: 635DepositorInsertFibrillarin (FBL Human)
UseTagsmKate1.3ExpressionMammalianMutationR36Q, S66FPromoterCMVAvailable sinceApril 1, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCEP4-HA-Flag-natT1R2 a22-end_I132S-S212N-D231G
Plasmid#113953Purposemammalian expression plasmid for FLAG-tagged human T1R2 with signal peptide of influenza hemagglutinin; expression-enhanced variantDepositorInsertT1R2 (TAS1R2 Human)
UseTagsFLAG and Signal/leader sequence from influenza he…ExpressionMammalianMutationdelete N-terminal 21 residues, I132S, S212N, and …PromotercmvAvailable sinceFeb. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMTS_mScarlet-H_N1
Plasmid#85058PurposeIn vivo visualization of the mitochondria (can be used for colocalization studies)DepositorInsertMTS (COX8A Human)
UseTagsmScarlet-HExpressionMammalianMutationaa1-29PromoterCMVAvailable sinceMarch 16, 2017AvailabilityAcademic Institutions and Nonprofits only -
pet21a-aGFPnb-enhancer-cys-linker-YbbR-(PAS)5-H6
Plasmid#192788PurposeaGFP nanobody enhancer tagged with cysteine, YbbR and H6 for bacterial expression, purification and labelingDepositorInsertantiGFP nanobody enhancer
UseTagsH6 and ybbRExpressionBacterialMutationPromoterAvailable sinceDec. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
mCardinal-H1-10
Plasmid#56161PurposeLocalization: Nucleus/Histones, Excitation: 604, Emission: 659DepositorInsertH1 (H1f0 Mouse)
UseTagsmCardinalExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 27, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pFRT-DHX36iso2E335A
Plasmid#159588PurposeExpress N-ter FLAG HA tagged DHX36 isoform 2 with E335A mutation in mammalian cellsDepositorInsertDHX36 (DHX36 Human)
UseTagsFLAG HAExpressionMammalianMutationE335APromoterCMVAvailable sinceJune 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
VAMP3(Δ71)-mCherry
Plasmid#92425PurposeExpression of fusion incompetent VAMP3 (del71) C-terminally conjugated to mCherry.DepositorInsertVAMP3 (Vamp3 Mouse)
UseTagsmCherryExpressionMammalianMutationdeleted leucine 71PromoterCMVAvailable sinceJuly 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
mTFP1-Farnesyl-5
Plasmid#55481PurposeLocalization: Plasma Membrane, Excitation: 462, Emission: 492DepositorInsertFarnesyl (HRAS Human)
UseTagsmTFP1ExpressionMammalianMutationencodes final 20 aa of NM_001130442.1PromoterCMVAvailable sinceOct. 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGEX-6P-1 AUP1 292-410
Plasmid#185331PurposeBacterial expression of AUP1 with GST fusion protein for binding assaysDepositorInsertAUP1 (AUP1 Human)
UseTagsGSTExpressionBacterialMutationaa 292-410 onlyPromoterAvailable sinceFeb. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-f:PGC1-3D
Plasmid#47DepositorInsertPGC-1a (Ppargc1a Mouse)
UseTagsflagExpressionMammalianMutationAmino acids 262, 265, and 298 have been changed t…PromoterAvailable sinceOct. 4, 2005AvailabilityAcademic Institutions and Nonprofits only -
pET21-10XHis-GST-HRV-Her2-dL5-Her2
Plasmid#85624PurposeBacterial expression vector for Z342-dL5(E52D)-Z342 affibody fusion protein (AFA) with N-terminal His10, GST and HRV3C cleavage site (MBIC5, dL5**, FAP, AffiFAP)DepositorInsert10XHis-GST-HRV-Her2-dL5-Her2 (EGFR Human, Schistosoma japonicum, Synthetic)
UseTags10XHis-GST-HRV and The Her2 Affibody and dL5 FAP …ExpressionBacterialMutationThe dL5** FAP is E50D, L89S, also known as E52D/L…PromoterT7Available sinceJuly 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGEX/PD-L1 3SA
Plasmid#121467PurposeExpression of GST-human PD-L1 in E. ColiDepositorInsertCD274 (CD274 Human)
UseTagsGSTExpressionBacterialMutationS176A, T180A, S184APromoterTacAvailable sinceFeb. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV-hARdelta120-472
Plasmid#89088Purposemammalian expression of human androgen receptor with deletion of 120-472 amino acid residuesDepositorInserthAR-120-472 N-term deletion (AR Human)
UseTagsExpressionMammalianMutationhAR-120-472PromoterCMVAvailable sinceApril 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
p3xFLAG-CMV TIP60 1-211
Plasmid#78794PurposeTo overexpress TIP60 1-211 in Mammalian CellsDepositorInsertTIP60 (KAT5 Human)
UseTags3xFLAGExpressionMammalianMutation1-211PromoterCMVAvailable sinceJune 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRRLHygro-pEF1a-p53ash-mKate2-splitmVenusC
Plasmid#69583PurposeExpresses p53 tagged with mKate2 and split C-term mVenusDepositorInsertp53 (TP53 Human)
UseLentiviralTagsmKate2 and split mVenus - C termExpressionMammalianMutation7 silent mutations in p53 DNA to prevent targetin…PromoterEF1alphaAvailable sinceNov. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-HA-Flag-natT1R2 ECD-MHC
Plasmid#113957Purposemammalian expression plasmid for FLAG-tagged human T1R2 ECD with signal peptide of influenza hemagglutinin fused to a canonical transmembrane domain from MHC class IDepositorInsertT1R2 (TAS1R2 Human)
UseTagsFLAG, Signal/leader sequence from influenza hemag…ExpressionMammalianMutationresidues 22-568 fused to a transmembrane tetherPromotercmvAvailable sinceFeb. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-f:PGC1-L2/3A
Plasmid#48DepositorInsertPGC-1a (Ppargc1a Mouse)
UseTagsflagExpressionMammalianMutationLXXLL at aa140 is changed to AXXLL; LLXXL at aa 2…PromoterAvailable sinceOct. 4, 2005AvailabilityAcademic Institutions and Nonprofits only -
pFUSEss-CHIg-mG1_Arg322Lys
Plasmid#105849PurposeExpression plasmid coding for mutated heavy chain of mouse IgG1 antibody specific to antigen B of the ABO blood group system, clone M18. Mutation: Arg322Lys (EU numbering).DepositorInsertIgG1 heavy chain (Ighg1 Mouse)
UseTagsExpressionMammalianMutationArg322Lys in constant region of IgG1 heavy chainPromoterhEF1-HTLVAvailable sinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMS04 [myo-3p::bPGC::SL2::mCherry]
Plasmid#168172PurposeExpression of bPGC in BWMs of C. elegansDepositorInsertbPGC
UseTagsExpressionWormMutationPromoterAvailable sinceMay 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-HA p21 K161R, K163R
Plasmid#78789PurposeTo overexpress p21 K161R, K163R in Mammalian CellsDepositorInsertp21 (CDKN1A Human)
UseTagsHAExpressionMammalianMutationK161R, K163R_muationPromoterCMVAvailable sinceJune 8, 2016AvailabilityAcademic Institutions and Nonprofits only