Addgene

We narrowed to 19,139 results for: Cre

Showing: 5081 - 5100 of 19139 results

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CMVTO-GFP-ECL-3TM-tevs-KKMP V3

Plasmid

#179640

Purpose

TEVP-inducible membrane-bound GFP RELEASE construct

Depositor

Xiaojing Gao

Article

Vlahos et al Nat Commun. 2022 Feb 17;13(1):912. doi:

Insert

Membrane-bound GFP RELEASE

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 23, 2022

Availability

Academic Institutions and Nonprofits only

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RpHLuorin2

Plasmid

#180227

Purpose

for bacterial expression of recombinant ratiometric pHluorin2

Depositor

Geert van den Bogaart

Article

Linders et al ACS Chem Biol. 2022 Jan 21;17(1):240-251

Insert

Ratiometric pHLuorin2

Use

Tags

His-tag followed by thrombin cleavage site

Expression

Bacterial

Mutation

Promoter

T7

Available since

March 4, 2022

Availability

Academic Institutions and Nonprofits only

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Str-Ii_VSVG-SBP-EGFP

Plasmid

#65300

Purpose

synchronize trafficking of VSVG from the ER (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

VSV-G

Use

Tags

EGFP and Streptavidin Binding Protein (SBP)

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 3, 2015

Availability

Academic Institutions and Nonprofits only

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5xGAL4-TATA-luciferase

Plasmid

#46756

Purpose

Luciferase reporter gene for factors with GAL4 DNA binding domains

Depositor

Richard Maurer

Article

Sun et al Genes Dev. 1994 Nov 1;8(21):2527-39.

Insert

Luciferase

Use

Luciferase

Tags

Expression

Mutation

Promoter

minimal TATA

Available since

Aug. 1, 2013

Availability

Academic Institutions and Nonprofits only

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BFP-Rab5

Plasmid

#49147

Purpose

mammalian expression of BFP-Rab5 fusion

Depositor

Gia Voeltz

Article

Friedman et al Mol Biol Cell. 2013 Apr;24(7):1030-40. d

Insert

Rab5 (RAB5B Human)

Use

Tags

BFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 29, 2014

Availability

Academic Institutions and Nonprofits only

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pLenti6.2_mTagBFP2

Plasmid

#113725

Purpose

Expresses the fluorescent protein mTagBFP2 in a lentiviral backbone

Depositor

Vanessa LaPointe

Article

Wieland et al Front Bioeng Biotechnol. 2021 Sep 9;9:72

Insert

mTagBFP2

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pMCB320

Plasmid

#89359

Purpose

3rd generation lentiviral sgRNA expression vector with mCherry, puromycin resistance, BlpI+BstXI cloning sites

Depositor

Michael Bassik

Article

Han et al Nat Biotechnol. 2017 Mar 20. doi: 10.103

Insert

puromycin resistance, mCherry

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 14, 2017

Availability

Academic Institutions and Nonprofits only

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pmNeonGreenHO-mito

Plasmid

#127913

Purpose

a mammalian expression vector for mitochondria-targeting humanized mNeonGreen

Depositor

Isei Tanida

Article

Tanida-Miyake et al PLoS One. 2018 Jan 17;13(1):e0191108. do

Insert

humanized mNeonGreen with mitocondrial targetting signal

Use

Tags

mNeonGreen

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pSFFV_Gal4-VP64-IRES-mCherry (pLZA139)

Plasmid

#203909

Purpose

Encodes wild type Gal4-VP64 transcription factor in mammalian cells

Depositor

Jared Toettcher

Article

Zhu et al Nat Commun. 2023 Jun 2;14(1):3185. doi:

Insert

Gal4-VP64-IRES-mCherry

Use

Tags

Expression

Mammalian

Mutation

Promoter

SFFV

Available since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pBA559

Plasmid

#186235

Purpose

pTet-LbuCas13a crRNA entry vector

Depositor

Jennifer Doudna

Article

Adler et al Nat Microbiol. 2022 Dec;7(12):1967-1979.

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pXPR_050

Plasmid

#96925

Purpose

lentiviral expression of gRNA scaffold

Depositor

John Doench, David Root

Article

Sanson et al Nat Commun. 2018 Dec 21;9(1):5416. doi:

Type

Empty backbone

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 28, 2017

Availability

Academic Institutions and Nonprofits only

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Ii-Str_SBP-mCherry-Ecadherin

Plasmid

#65289

Purpose

synchronize trafficking of Ecadherin from the ER (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

Ecadherin

Use

Tags

IL-2 Signal Sequence and Streptavidin Binding Pro…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 3, 2015

Availability

Academic Institutions and Nonprofits only

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Ii-Str_SBP-EGFP-Ecadherin

Plasmid

#65288

Purpose

synchronize trafficking of Ecadherin from the ER (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

Ecadherin

Use

Tags

EGFP and IL-2 Signal Sequence and Streptavidin Bi…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 3, 2015

Availability

Academic Institutions and Nonprofits only

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PGRP-SA

Plasmid

#21055

Depositor

Johann Deisenhofer

Article

Chang et al PLoS Biol. 2004 Sep . 2(9):E277.

Insert

PGRP-SA (PGRP-SA Fly)

Use

Tags

His

Expression

Insect

Mutation

Promoter

Available since

Sept. 8, 2009

Availability

Academic Institutions and Nonprofits only
pPB-R1R2-EM7neoPheS

Plasmid

#112923

Purpose

piggyBac Transposon vector with a Gateway destination cassette (neoPheS selection)

Depositor

Kosuke Yusa

Article

Chong et al Genome Biol. 2018 Nov 26;19(1):205. doi:

Insert

neoPheS

Use

Piggybac transposon gateway destination vector

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pIVEX-SNAP-GFP

Plasmid

#46846

Purpose

SNAP-GFP fusion

Depositor

Florian Hollfelder

Article

Diamante et al Protein Eng Des Sel. 2013 Oct;26(10):713

Insert

SNAP-GFP fusion

Use

In vitro expression

Tags

Expression

Mutation

Promoter

Available since

Aug. 12, 2013

Availability

Academic Institutions and Nonprofits only

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pENTR2b-mTurquoise2

Plasmid

#191014

Purpose

Gateway entry vector with the mTurquoise2 fluorescent protein

Depositor

Johanna Ivaska

Article

Conway et al Sci Adv. 2023 Jul 14;9(28):eadg1840. doi

Insert

mTurquoise2

Use

Gateway entry vector

Tags

Expression

Mutation

Promoter

Available since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pDYU1G/γ-tubulin-mEGFP

Plasmid

#154301

Purpose

Expresses γ-tubulin–mEGFP from actin15 promoter in Dictyostelium discoideum

Depositor

Shigehiko Yumura

Article

Kondo et al Biotechniques. 2020 Feb;68(2):91-95. doi

Insert

γ-tubulin

Use

Dictyostelium expression

Tags

mEGFP

Expression

Mutation

Promoter

actin15

Available since

Jan. 29, 2021

Availability

Academic Institutions and Nonprofits only

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ATeam1.03-nD/nA/pcDNA3

Plasmid

#51958

Purpose

mammalian expression of ATeam1.03-nD/nA, a FRET-based ATP indicator

Depositor

Takeharu Nagai

Article

Kotera et al ACS Chem Biol. 2010 Feb 19;5(2):215-22.

Insert

ATeam1.03-nD/nA

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 28, 2014

Availability

Academic Institutions and Nonprofits only

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pMT_025

Plasmid

#158579

Purpose

Lentiviral expression of a customizable open reading frame

Depositor

John Doench, David Root

Article

Hanna et al Cell. 2021 Feb 18;184(4):1064-1080.e20.

Type

Empty backbone

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

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