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Plasmid#60618PurposeEncodes MYC tagged beta-actin with a mutation in the NESDepositorInsertBeta-actin (ACTB Human)
UseTagsMYCExpressionBacterial and MammalianMutationI178A, L180APromoterAvailable sinceNov. 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T3-ZO-2-1st-PDZ
Plasmid#27424DepositorInsertZonula Occludens 2 (TJP2 Human)
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceMarch 31, 2011AvailabilityAcademic Institutions and Nonprofits only -
pEBB-GFP-hBEN-deltaNLS3
Plasmid#22158DepositorInserthBEN (GTF2IRD1 Human)
UseTagsGFPExpressionMammalianMutationDeletion of amino acids 883-889PromoterAvailable sinceNov. 13, 2009AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4/HisMaxB-YAP2-deltaC
Plasmid#21124DepositorArticleInsertYes-kinase associated protein (YAP1 Human)
UseTagsHisExpressionMammalianMutation5 Carboxy-terminal amino acids -FLTWL are deletedPromoterAvailable sinceJuly 1, 2009AvailabilityAcademic Institutions and Nonprofits only -
pWay21-MKP-1CH2A
Plasmid#13473DepositorInsertMKP-1CH2A (Dusp1 Mouse)
UseTagsEGFPExpressionMammalianMutationContains aa 1-46PromoterAvailable sinceDec. 8, 2006AvailabilityAcademic Institutions and Nonprofits only -
pWay21-MKP-1CH2B
Plasmid#13474DepositorInsertMKP-1CH2B (Dusp1 Mouse)
UseTagsEGFPExpressionMammalianMutationContains aa 47-136PromoterAvailable sinceDec. 8, 2006AvailabilityAcademic Institutions and Nonprofits only -
pCAGEN_N73_eMagBF_V5
Plasmid#235642PurposeEncodes ALIBi N-terminal fragment, residues 1-73, fused to eMagBF (enhanced Magnet, basic, fast), V5. No nuclear export sequence to allow nuclear localization.DepositorInsertALIBi N-terminal fragment
UseTagseMagBF-V5ExpressionMammalianMutationresidues 1-73PromoterAvailable sinceApril 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET28a-Pchi-HMG
Plasmid#231540PurposeExpresses the HMG of Pchi Sox with a 6xHis tag for protein purificationDepositorInsertSox HMG
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceMarch 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
Perturb-FISH
Plasmid#220626Purposeexpression of gRNAs under U6T7 promoterDepositorInsertsgRNA
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterBsmBIAvailable sinceMarch 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
pETG20a-Pchi-HMG
Plasmid#231538PurposeExpresses the HMG of Pchi Sox, for protein purificationDepositorInsertSox HMG
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceMarch 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
VirTREX2-HLDel-1_NbmiR164e/h
Plasmid#231156PurposeT-DNA encoding TRV2 with TREX2 and mobile gRNA targeting NbmiR164e/hDepositorInsertTREX2 and mobile gRNA targeting NbmiR164e/h
UseTagsExpressionPlantMutationPromoterPEBV sub-genomicAvailable sinceMarch 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
VirTREX2-HLDel-1_SlMIR164b
Plasmid#231152PurposeT-DNA encoding TRV2 with TREX2 and mobile gRNA targeting SlMIR164bDepositorInsertTREX2 and mobile gRNA targeting SlMIR164b
UseTagsExpressionPlantMutationPromoterPEBV sub-genomicAvailable sinceMarch 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
esgRNA_NbATML1-1pro
Plasmid#231151PurposeT-DNA encoding TRV2 with mobile gRNA targeting NbATML1-1proDepositorInsertmobile gRNA targeting NbATML1-1pro
UseTagsExpressionPlantMutationPromoterPEBV sub-genomicAvailable sinceMarch 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
esgRNA_NbSTM-5'UTR
Plasmid#231150PurposeT-DNA encoding TRV2 with mobile gRNA targeting NbSTM-5?UTRDepositorInsertmobile gRNA targeting NbSTM-5?UTR
UseTagsExpressionPlantMutationPromoterPEBV sub-genomicAvailable sinceMarch 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
VirTREX2-HLDel-1_NbPDS
Plasmid#231146PurposeT-DNA encoding TRV2 with TREX2 and mobile gRNA targeting NbPDSDepositorInsertTREX2 and mobile gRNA targeting NbPDS
UseTagsExpressionPlantMutationPromoterPEBV sub-genomicAvailable sinceMarch 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
VirTREX2-HLDel-2_NbPDS
Plasmid#231145PurposeT-DNA encoding TRV2 with TREX2 and mobile gRNA targeting NbPDSDepositorInsertTREX2 and mobile gRNA targeting NbPDS
UseTagsExpressionPlantMutationPromoterPEBV sub-genomicAvailable sinceMarch 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-Twin-strep-TEV-DGKδ2
Plasmid#223720PurposeExpress human diacylglycerol kinase delta 2 protein (N-terminal Tobacco Etch Virus (TEV) protease cleavable Twin-Strep-fusion protein (ENLYFQGS-WSHPQFEK-(GGGS)2-GGSA-WSHPQFEK)) in mammalian cellDepositorInsertDGKD (DGKD Human)
UseTagsTEV cleavable site and Twin-Strep-tagExpressionMammalianMutationdeleted start codonPromoterCAG and chicken β-actin promoterAvailable sinceJan. 8, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-PHOSPHO1-TEV-Twin-Strep
Plasmid#222015PurposeExpress human PHOSPHO1 protein (C-terminal Tobacco Etch Virus (TEV) protease cleavable Twin-Strep-fusion protein (ENLYFQGS-WSHPQFEK-(GGGS)2-GGSA-WSHPQFEK)) in mammalian cellDepositorUseTagsTEV cleavable site and Twin-Strep-tagExpressionMammalianMutationstop codon (TGA) is deletedPromoterchicken β-actin promoterAvailable sinceJan. 8, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLentiCRISPRRv2_sgTom
Plasmid#218346PurposeCRISPR KO plasmid for tdTomato in human cell linesDepositorInsertInserted sgRNA targetting tdTomato
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceDec. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFastBac SOX 318-320A
Plasmid#192060PurposeExpression vector for SOX (ORF37) 318-320ADepositorInsertSOX (ORF37)
UseTagsExpressionInsectMutation318-320APromoterPolyhedrinAvailable sinceDec. 4, 2024AvailabilityAcademic Institutions and Nonprofits only