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Plasmid#211815Purposelentiviral vector expressing SpCas9 and tracrRNA for stable cell line establishmentDepositorInsertSpCas9, tracrRNA and puromycin selection gene
UseLentiviralTagsExpressionMammalianMutationPromoterU1a promoter, U6 promoter, and hPGK promoterAvailable sinceJan. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
Donor-CD55-NeoR
Plasmid#153551PurposeUsed as a donor vector for the targeted knock-in of a CD55 mutation. This plasmid carries a neomycin resistance gene cassetteDepositorInsertmutant CD55 (a 1415-bp fragment from intron 3, exon 4 and intron 4, with a 1-bp mutation in exon 4), divided into 5' and 3' arms
UseAAV and Cre/Lox; Donor plasmid/targeting vectorTagsN/AExpressionMutationa 1-bp mutation in exon 4PromoterNo promoterAvailable sinceMarch 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
Donor-PIGA-NeoR
Plasmid#153549PurposeUsed as a donor vector for the targeted knock-in of a PIGA mutation. This plasmid carries a neomycin resistance gene cassetteDepositorInsertmutant PIGA (a 1,991-bp fragment from intron 5 and exon 6 with a 3-bp mutation in exon 6), divided into 5' and 3' arms
UseAAV and Cre/Lox; Donor plasmid/targeting vectorTagsN/AExpressionMutationa 3-bp mutation in exon 6PromoterNo promoterAvailable sinceDec. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
HL 6279
Bacterial Strain#52949PurposeMG1655 + fimS (off orientation)::gfpAAV tag::Asp terminator::CamR at fimA + ΔlacI + KanR replaces fimB and fimE(partial)DepositorBacterial ResistanceKanamycinSpeciesEscherichia coliAvailable sinceApril 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
sgCRY1
Plasmid#176511PurposeExpresses sgRNA in mammalian cellsDepositorUseAAVTagsmCherry (K14E, N210D, E249G)ExpressionMutationPromoterU6, hSynAvailable sinceJan. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
px552-U6-gRNA1-U6-gRNA2-CMV-eGFP
Plasmid#211760PurposePaired gRNAs (sgRNA1 and 2) targeting Exon 1 of VEGFA gene conserved across mouse, rhesus macaque, and human.DepositorInsertgRNA1 and gRNA2 targeting VEGF-A (VEGFA Human, Mouse, M. mulatta (rhesus macaque))
UseAAV and CRISPRTagsExpressionMammalianMutationPromoterU6Available sinceFeb. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
px552-U6-gRNA1-CMV-eGFP
Plasmid#211758PurposesgRNA1 targeting Exon 1 of VEGFA gene conserved across mouse, rhesus macaque, and human.DepositorInsertgRNA1 targeting VEGF-A (VEGFA Human, Mouse, M. mulatta (rhesus macaque))
UseAAV and CRISPRTagsExpressionMammalianMutationPromoterU6Available sinceFeb. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
px552-U6-gRNA2-CMV-eGFP
Plasmid#211759PurposesgRNA2 targeting Exon 1 of VEGFA gene conserved across mouse, rhesus macaque, and human.DepositorInsertgRNA2 targeting VEGF-A (VEGFA Human, Mouse, M. mulatta (rhesus macaque))
UseAAV and CRISPRTagsExpressionMammalianMutationPromoterU6Available sinceFeb. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
Antibody#196562-rAbPurposeAnti-6xHis chimeric recombinant antibody. The hybridoma-derived heavy chain variable sequence and kappa light chain are fused to a rabbit IgG Fc.DepositorRecommended ApplicationsWestern BlotReactivitySyntheticSource SpeciesRabbitIsotypeIgGTrial SizeAvailable to purchaseAvailable sinceFeb. 24, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits
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UAS-mCherry
Plasmid#135457PurposeMammalian mCherry reporter to read out FLARE and SPARK activationDepositorInsertmCherry
UseTagsmCherryExpressionMammalianMutationPromoterUASAvailable sinceJan. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRU1099
Plasmid#14464DepositorInsertunstable gfp-mut3.1 AAV
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 23, 2007AvailabilityAcademic Institutions and Nonprofits only -
pHL 1571
Plasmid#37760DepositorInsertsptet_ompC_gfpAaV
pLac_st3 TetR_mCherry
UseSynthetic Biology; E. coliTagsgfp and mCherryExpressionMutationAaV degradation tag added to the 3'endPromoterpLac and ptetAvailable sinceAug. 10, 2012AvailabilityAcademic Institutions and Nonprofits only -
pHL 1569
Plasmid#37759DepositorInsertsptet_ompC_gfpAaV
pLac_micC_mCherry
UseSynthetic Biology; E. coliTagsgfp and mCherryExpressionMutationAaV degradation tag added to the 3'endPromoterpLac and ptetAvailable sinceJuly 27, 2012AvailabilityAcademic Institutions and Nonprofits only -
pSLED.GluA2
Plasmid#193017PurposeGluA2 (Gria2) flip/flop reporter (hSyn promoter, Gria2 flip/flop exons, bichromatic reporter)DepositorInsertBichromatic reporter (EGFP and dsRed-Express2)
UseAAVTagsExpressionMutationPromoterAvailable sinceDec. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSLED.ENS
Plasmid#193016PurposeExcitatory neuron-specific reporter (hSyn promoter, Synrg exon, bichromatic reporter)DepositorInsertBichromatic reporter (EGFP and dsRed-Express2)
UseAAVTagsExpressionMutationPromoterAvailable sinceDec. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSLED.NPL
Plasmid#193014PurposeNeuron-specific reporter (CBh promoter, Pls3 exon, bichromatic reporter)DepositorInsertBichromatic reporter (EGFP and dsRed-Express2)
UseAAVTagsExpressionMutationPromoterAvailable sinceDec. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSLED.CaRPv1
Plasmid#193018PurposeExcitatory/Inhibitory neuron calcium indicator (hSyn promoter, Synrg exon, jRGECO1a/jGCaMP7b)DepositorInsertBichromatic calcium indicator (jGCaMP7b and jRGECO1a)
UseAAVTagsExpressionMutationPromoterAvailable sinceDec. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSLED.RAB
Plasmid#193015PurposePhotoreceptor-specific reporter (CBh promoter, Atp1b2 exon, bichromatic reporter)DepositorInsertBichromatic reporter (EGFP and dsRed-Express2)
UseAAVTagsExpressionMutationPromoterAvailable sinceDec. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
CMV:eGFP-CaM-uTEV1Δ(220-242)
Plasmid#135461PurposeMammalian expression of uFLARE protease componentDepositorInserteGFP-CaM-uTEV1Δ
UseTagseGFP, V5ExpressionMammalianMutationS219V mutation improves stability and S153N impro…PromoterCMVAvailable sinceJan. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pVH001
Plasmid#80394PurposeContains aTc inducible Scaffold-mCherry-AAV and TetR genesDepositorInsertmCherry RFP
UseSynthetic BiologyTagsFused by GS linkers to Sh3pep-LZX scaffold protei…ExpressionBacterialMutationPromoterAvailable sinceSept. 2, 2016AvailabilityAcademic Institutions and Nonprofits only