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Plasmid#143971PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertFOXE1 (FOXE1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceMay 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
3'-ecRESCUE-mCherry
Plasmid#191486PurposeecDHFR DD-mCherry, an inducible ecRESCUE RNA base editor: TMP-induced ecDHFR DD expression allows reversible control of the expression of dRanCas13b-ADAR2-mCherryDepositorUseCRISPRTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pIG-703pUC19-tNGFR-P2A-BACH2
Plasmid#186112PurposeKnockin of truncated NGFR to target geneDepositorInsertBACH2 (BACH2 Human)
UseCRISPRTagsExpressionMutationWT with tNGFR sequencePromoterAvailable sinceJuly 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSICO-CPSF6-4Glu-iRFP670
Plasmid#167588PurposeExpresses a mutant form of CPSF6 (CPSF6-4Glu described in Jang et al. Nucleic Acids Res 2019) fused to iRFP670DepositorInsertCPSF6-4Glu-iRFP670 (CPSF6 Human)
UseLentiviralTagsiRFP670ExpressionMutationK547E, R549E, R559E, R561EPromoterAvailable sinceApril 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBabe-Puro-cRAF-W22
Plasmid#15270DepositorInsertCRAF (RAF1 Human)
UseRetroviralTagsExpressionMammalianMutationC-terminal deletionPromoterAvailable sinceJuly 12, 2007AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-PIK3CD
Plasmid#116773PurposeLentiviral expression of PIK3CDDepositorInsertPIK3CD (PIK3CD Human)
UseLentiviralTagsExpressionMutationWTPromoterAvailable sinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
DHC1-AID-Clover donor (Hygro)
Plasmid#158622PurposeDonor plasmid for tagging DHC1 with AID-CloverDepositorInsertDHC1 (DYNC1H1 Human)
UseCRISPR; Tagging donorTagsExpressionMutationPromoterAvailable sinceNov. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLJM5-mSHMT2
Plasmid#83905Purposestable overexpressionDepositorInsertSerine hydroxymethyltransferase 2 (Shmt2 Mouse)
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLKO_S.NWS.VSVg_NGFR
Plasmid#158348PurposeProtein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).DepositorInsertPro-Code Tagged human dNGFR (NGFR Human)
UseCRISPR and LentiviralTagsS.NWS.VSVgExpressionMutationTruncation of the signaling domainPromoterEF1aAvailable sinceSept. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
CRY2high-mCherry-Raf1
Plasmid#104064PurposeExpresses fusion of CRY2high mutant (CRY2PHR E490R) with mCherry and Raf1DepositorUseTagsExpressionMammalianMutationCRY2PHR E490RPromoterCMVAvailable sinceDec. 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
PLL5.0-Anillin ShRNA-Cherry-Anillin deltaActin binding domain
Plasmid#187276PurposeLentiviral expression of shRNA targeting Anillin + reconstitution of Anillin delta Actin binding domainDepositorInsertAnillin ,hsAnillin, Entrez Gene ANLN (a.k.a. FSGS8, Scraps, scra) (ANLN Human)
UseLentiviralTagsmCherryExpressionMutationdeltaActin binding domainPromoterU6, CMVAvailable sinceSept. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-Golgi-RasAR
Plasmid#205570PurposeGolgi membrane-targeted RasAR.DepositorUseTagsGolgin B1 (Giantin) residues 3131-3259ExpressionMammalianMutationPromoterCMVAvailable sinceSept. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
ATX3-13Q
Plasmid#184248PurposeRecombinant protein expression and purification. Encodes ataxin-3 full length with 3 UIMs and 13Q in the Poly-Q track fused with His-Tag and TEV cleavage sequence (N-terminal)DepositorInsertataxin-3 full length with 3 UIMs and 13Q in the Poly-Q track (ATXN3 Synthetic, Human)
UseTags6x His-Tag and TEV cleavage siteExpressionBacterialMutationC-terminal codon optimization for protein expres…PromoterT7 promoterAvailable sinceAug. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
EGFP-Bcr R1090A
Plasmid#36416DepositorInsertbreakpoint cluster region protein isoform 1 (BCR Human)
UseTagsEGFPExpressionMammalianMutationR1090APromoterAvailable sinceAug. 30, 2012AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-SLC27A3_STOP
Plasmid#161282PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.DepositorInsertSLC27A3 (SLC27A3 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 15, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
PLL5.0-Anillin ShRNA-Cherry-Anillin deltaMyosin binding domain
Plasmid#187275PurposeLentiviral expression of shRNA targeting Anillin + reconstitution of Anillin delta Myosin binding domainDepositorInsertAnillin ,hsAnillin, Entrez Gene ANLN (a.k.a. FSGS8, Scraps, scra) (ANLN Human)
UseLentiviralTagsmCherryExpressionMutationdeltaMyosin binding domainPromoterU6, CMVAvailable sinceSept. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCIBN-tagBFP-hLaminB1
Plasmid#103799PurposeBLInCR 'Localizer' construct that marks the nuclear lamina and is targeted by a PHR-tagged effector upon illumination with blue lightDepositorUseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCIBN-EYFP-hTRF1
Plasmid#103802PurposeBLInCR 'Localizer' construct that marks the telomeres and is targeted by a PHR-tagged effector upon illumination with blue lightDepositorUseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSpCas9_human_ADAR2_ccC
Plasmid#158112Purposedeletion hADAR2DepositorInsertADAR2 (ADARB1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSpCas9_human_ADAR2_ccB
Plasmid#158113Purposedeletion hADAR2DepositorInsertADAR2 (ADARB1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 4, 2022AvailabilityAcademic Institutions and Nonprofits only