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Plasmid#108656PurposeA JNK biosensor based on fluorescence resonance energy transfer (FRET) and those based on bioluminescence energy transfer (BRET)DepositorInserthyBRET-JNK-EV
UseTagsXFPExpressionMammalianMutationPromoterAvailable sinceApril 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRK5-myc-Miro1 Δ593-618
Plasmid#47895PurposeExpresses myc tagged Miro1 lacking transmembrane domainDepositorInsertMiro1 Δ593-618 (RHOT1 Human)
UseTagsmycExpressionMammalianMutationΔ593-618, lacks transmembrane domainPromoterCMVAvailable sinceSept. 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET28a-GGG-ELP(120nm)-Cys
Plasmid#91572PurposeElastin Like Polypeptide: [(VPGEG)-(VPGVG)4-(VPGAG)2-(VPGGG)2-(VPGEG)]6, with N-Terminal Sortase Recognition Sequence GGG and a C-terminal CysteineDepositorInsertGGG-ELP(120nm)-Cys
UseTagsCysteine and Sortase-TagExpressionBacterialMutationPromoterT7Available sinceJuly 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMRE-Tn7-163
Plasmid#118575PurposeminiTn7 plasmid to deliver constitutively expressed fluorescent protein genes in bacteriaDepositorInsertsYFP2
UseMinitn7 delivery vectorTagsExpressionBacterialMutationPromoterAvailable sinceOct. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGL4.13/AUG-FFLuc-3XFLAG
Plasmid#127333PurposeExpress 3xFLAG tagged firefly luciferase (FFLuc) from AUG start codonDepositorInsertFirefly luciferase
UseTags3xFLAGExpressionMammalianMutationPromoterCV40Available sinceAug. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMV-FLAG TIF1gamma M (456-887)
Plasmid#15735DepositorInsertTIF1 gamma M (TRIM33 Human)
UseTagsFlagExpressionMammalianMutationMiddle region (amino acids 456-887).PromoterAvailable sinceSept. 7, 2007AvailabilityAcademic Institutions and Nonprofits only -
pMRE-Tn5-141
Plasmid#118525PurposeminiTn5 plasmid to deliver constitutively expressed fluorescent protein genes in bacteriaDepositorInsertmTurquoise2
UseMinitn5 delivery vectorTagsExpressionBacterialMutationPromoterAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pClgn1.1-Trim41-PA-1D4
Plasmid#186339PurposeExpress Trim41-PA-1D4 under the mouse Clgn promoter.DepositorInsertTrim41 (Trim41 Mouse)
UseTags1D4 and PAExpressionMammalianMutationPromoterpClgn1.1Available sinceJuly 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGSTag Flag Jnk2a2
Plasmid#15744DepositorInsertJnk2a2 (MAPK9 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 6, 2008AvailabilityAcademic Institutions and Nonprofits only -
pQCXIN-EF1a-mNeonGreen-P2A-Cas9
Plasmid#189807PurposeRetroviral delivery of Cas9DepositorInsertCas9
UseRetroviralTagsExpressionMutationPromoterAvailable sinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
POLArIS_NbVim-cp10GFP
Plasmid#171020PurposeExpresses NbVim-cp10GFP#2 in mammalian cellsDepositorInsertanti-vimentin-nanobody VB6 (NbVim) tagged with circularly permutated superfolderGFP(cp10GFP)
UseTagscp10GFPExpressionBacterial and MammalianMutationPromoterCMV promoterAvailable sinceJune 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
CAGhP14
Plasmid#84362Purposeexpress FLAG-HA tagged human PRDM14DepositorInsertPRDM14 (PRDM14 Human)
UseTagsFLAG and HAExpressionMammalianMutationsilent mutations at coding silent 1068CtoT, 1629T…PromoterCAGAvailable sinceFeb. 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLck-mVenus-C1
Plasmid#84337PurposeExpresses a mVenus tagged with a membrane localization sequenceDepositorInsertLck (MGCVCSSNPELPVAT)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceDec. 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMRE-Tn7-166
Plasmid#118578PurposeminiTn7 plasmid to deliver constitutively expressed fluorescent protein genes in bacteriaDepositorInsertmCardinal
UseMinitn7 delivery vectorTagsExpressionBacterialMutationPromoterAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
uORF1-Mreinit- 36xMashTag-BFP-3xStop-24xPP7 (uORF-MashTag reporter)
Plasmid#128609PurposeExpresses uORF1 -36xMashTag with MoonTag as readout for reinitiation, followed by BFP linker and 24 repeats of PP7 hairpins in 3’ UTR.DepositorInsertuORF1-36xMashTag
UseTagsExpressionMammalianMutationPromoterPcmvAvailable sinceNov. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
CMV10-3xFlag Skp2 delta-F
Plasmid#81116Purposemammalian expression of F-box deleted Skp2DepositorInsertSKP2 (SKP2 Human)
UseTags3xFlagExpressionMammalianMutationdeleted F boxPromoterCMVAvailable sinceSept. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pME-ReaChR-citrine
Plasmid#169045PurposeMiddle entry vector for Gateway cloning that contains a ReaChR fused with citrine gene insertDepositorInsertReaChR-citrine
UseGateway entry cloning vectorTagscitrineExpressionMutationPromoterAvailable sinceJan. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
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tet-pLKO shGATA6 puro
Plasmid#72615PurposeExpresses an inducible short hairpin targeting human GATA6 sequenceDepositorInsertshort hairpin GATA binding protein 6
UseLentiviralTagsNoneExpressionMammalianMutationPromoterTRE TightAvailable sinceAug. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMRE-Tn7-142
Plasmid#118558PurposeminiTn7 plasmid to deliver constitutively expressed fluorescent protein genes in bacteriaDepositorInsertsGFP2
UseMinitn7 delivery vectorTagsExpressionBacterialMutationPromoterAvailable sinceOct. 6, 2023AvailabilityAcademic Institutions and Nonprofits only