Addgene

We narrowed to 14,973 results for: grna

Showing: 5021 - 5040 of 14973 results

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pAC-U63-tgRNA-Gal80

Plasmid

#169030

Purpose

gRNA-marker vector contain a U6:3 promoter, gRNA scaffold, and a Ubi-Gal80 marker.

Depositor

Chun Han

Article

Allen et al PLoS Biol. 2021 Jan 14;19(1):e3001061. d

Type

Empty backbone

Use

CRISPR

Tags

Expression

Insect

Mutation

Promoter

U6:3

Available since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

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pVD1 tRNA-gRNA E4-En-1 (GB2243)

Plasmid

#160565

Purpose

tRNA and scaffold for the assembly of GBoligomers for the position [4_(n-1)] of a polycistronic tRNA-gRNA regulated by the U6-26 or U6-1 promoter.

Depositor

Diego Orzaez

Article

Vazquez-Vilar et al Front Plant Sci. 2021 Jul 1;12:689937. d

Insert

tRNA-gRNA position E4-En-1 (Multiplexing Edit)

Use

CRISPR and Synthetic Biology

Tags

Expression

Mutation

BsaI and BsmBI sites removed

Promoter

Available since

March 24, 2021

Availability

Academic Institutions and Nonprofits only

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1026L=U6-3-gRNA#1[Pol-γ35]

Plasmid

#159674

Purpose

Plasmid supports expression of gRNA targeting Pol-γ35 site #1, tagged with mini-White, and can be integrated with phC31.

Depositor

Omar Akbari

Article

Kandul et al Elife. 2021 Mar 5;10. pii: 65939. doi: 1

Insert

U6-3-gRNA#1[Pol-γ35]

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

March 8, 2021

Availability

Academic Institutions and Nonprofits only

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1026M=U6-3-gRNA#2[Pol-γ35]

Plasmid

#159675

Purpose

Plasmid supports expression of gRNA targeting Pol-γ35 site #2, tagged with mini-White, and can be integrated with phC31.

Depositor

Omar Akbari

Article

Kandul et al Elife. 2021 Mar 5;10. pii: 65939. doi: 1

Insert

U6-3-gRNA#2[Pol-γ35]

Use

CRISPR

Tags

Expression

Insect

Mutation

Promoter

Available since

March 8, 2021

Availability

Academic Institutions and Nonprofits only

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pNTI763 p(Z)-mCherry + UBC9 sgRNA

Plasmid

#164918

Purpose

CEN/ARS with P(Z)-mCherry controlled by the ZEM transcription factor, also encode UBC9 sgRNA

Depositor

Nicholas Ingolia

Article

Muller et al Science. 2020 Dec 11;370(6522). pii: 370

Insert

P(Z)-mcherry

Use

CRISPR

Tags

Expression

Yeast

Mutation

Promoter

P(Z), synthetic promoter

Available since

Feb. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pSEM254 - sgRNA mosTI neoR

Plasmid

#159831

Purpose

Single copy or array insertion by MosTI using split NeoR selection

Depositor

Christian Froekjaer-Jensen

Article

El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

Insert

sgRNA mosTI neoR

Use

Tags

Expression

Worm

Mutation

Not applicable

Promoter

Available since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pBbdCas9S(-10AC1)_Psyn-sgRNArodA

Plasmid

#149659

Purpose

all-in-one CRISPRi vector for targeting B. burgdorferi rodA

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Insert

dCas9, lacI, sgRNArodA

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

PflaB, PpQE30(-10AC1), Psyn

Available since

Nov. 30, 2020

Availability

Academic Institutions and Nonprofits only

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pBbdCas9S(-10AC2)_Psyn-sgRNArodA

Plasmid

#149660

Purpose

all-in-one CRISPRi vector for targeting B. burgdorferi rodA

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Insert

dCas9, lacI, sgRNArodA

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

PflaB, PpQE30(-10AC2), Psyn

Available since

Nov. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pBbdCas9S(-10TC)_Psyn-sgRNAmreB

Plasmid

#149595

Purpose

all-in-one CRISPRi vector for targeting B. burgdorferi mreB

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Insert

dCas9, lacI, sgRNAmreB

Use

Tags

Expression

Bacterial

Mutation

Promoter

PflaB, PpQE30(-10TC), Psyn

Available since

Nov. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pBbdCas9S(-10TC)_Psyn-sgRNAftsI

Plasmid

#149591

Purpose

all-in-all CRISPRi vector for targeting B. burgdorferi ftsI

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Insert

dCas9, lacI, sgRNAftsI

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

PflaB, PpQE30(-10TC), Psyn

Available since

Nov. 24, 2020

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(gKAT6A-A8)-PGKpuro2ABFP-W

Plasmid

#159290

Purpose

Lentiviral vector expressing gRNA targeting KAT6A (gRNA ID. A8)

Depositor

Kosuke Yusa

Article

Au et al Leukemia. 2021 Apr;35(4):1012-1022. doi:

Insert

guide RNA targeting KAT6A (ID. A8) (KAT6A Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

human U6 promoter

Available since

Nov. 5, 2020

Availability

Academic Institutions and Nonprofits only

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pBbdCas9S(-10TC)_Psyn-sgRNArodA

Plasmid

#149657

Purpose

all-in-one CRISPRi vector for targeting B. burgdorferi rodA

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Insert

dCas9, lacI, sgRNArodA

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

PflaB, PpQE30(-10TC), Psyn

Available since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pBbdCas9S(-10TC)_Psyn-sgRNAflaB

Plasmid

#149589

Purpose

all-in-one CRISPRi vector for targeting B. burgdorferi flaB

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Insert

dCas9, lacI, sgRNAflaB

Use

Tags

Expression

Bacterial

Mutation

Promoter

PflaB, PpQE30(-10TC), Psyn

Available since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(gKAT6A-A7)-PGKpuro2ABFP-W

Plasmid

#159289

Purpose

Lentiviral vector expressing gRNA targeting KAT6A (gRNA ID. A7)

Depositor

Kosuke Yusa

Article

Au et al Leukemia. 2021 Apr;35(4):1012-1022. doi:

Insert

guide RNA targeting KAT6A (ID. A7) (KAT6A Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

human U6 promoter

Available since

Oct. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pKLV-gRNA-Bcan-Ntrk1_4

Plasmid

#136413

Purpose

Lentiviral expression of gRNAs targeting intron 13 of murine Bcan and intron 10 of murine Ntrk1. Also constitutively expresses Puromycin linked to TagBFP.

Depositor

Massimo Squatrito

Article

Oldrini et al Nat Commun. 2018 Apr 13;9(1):1466. doi:

Insert

U6_sgRNA(Bcan)_U6_sgRNA(Ntrk1)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Aug. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pKLV-gRNA-Myb-Qk_1

Plasmid

#136415

Purpose

Lentiviral expression of gRNAs targeting intron 4 of murine Qk and intron 9 of murine Myb. Also constitutively expresses Puromycin linked to TagBFP.

Depositor

Massimo Squatrito

Article

Oldrini et al Nat Commun. 2018 Apr 13;9(1):1466. doi:

Insert

U6_sgRNA(Myb)_U6_sgRNA(Qk)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Aug. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pKLV-gRNA-BTRC-MGMT_2

Plasmid

#136416

Purpose

Lentiviral expression of gRNAs targeting intron 2 of human BTRC and intron 1 of human MGMT. Also constitutively expresses Puromycin fused to TagBFP.

Depositor

Massimo Squatrito

Insert

U6_sgRNA(BTRC)_U6_sgRNA(MGMT)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Aug. 10, 2020

Availability

Academic Institutions and Nonprofits only

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px335-sgRNA-EF(Cas9n)-Ddx42-L

Plasmid

#122343

Purpose

Expresses sgRNA targeting mouse Ddx42 and Cas9 nickase in mammalian cells

Depositor

Hiroshi Ochiai

Article

Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

Insert

sgRNA for mouse Ddx42

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 30, 2020

Availability

Academic Institutions and Nonprofits only

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px335-sgRNA-EF(Cas9n)-Ddx42-R

Plasmid

#122344

Purpose

Expresses sgRNA targeting mouse Ddx42 and Cas9 nickase in mammalian cells

Depositor

Hiroshi Ochiai

Article

Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

Insert

sgRNA for mouse Ddx42

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 30, 2020

Availability

Academic Institutions and Nonprofits only

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px335-sgRNA-EF(Cas9n)-Ncapd3-R

Plasmid

#122330

Purpose

Expresses sgRNA targeting mouse Ncapd3 and Cas9 nickase in mammalian cells

Depositor

Hiroshi Ochiai

Article

Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

Insert

sgRNA for mouse Ncapd3

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 30, 2020

Availability

Academic Institutions and Nonprofits only

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