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Showing: 5001 - 5020 of 14727 results

  11. pETM11-6xHis-TEV-hORF1p_StammerAAA

    Plasmid
    #202587
    Purpose
    Allows for IPTG-inducible expression of the human L1RP ORF1 protein with the M91A, E92A, and L93A mutations in bacteria with an N-terminal 6xHis tag and TEV cleavage site for purification
    Depositor
    Liam Holt
    Article
    Sil et al Elife. 2023 Apr 28;12:e82991. doi: 10.75
    Insert
    ORF1p
    Use
    Tags
    6x His and TEV protease cleavage site
    Expression
    Bacterial
    Mutation
    Changed ORF1p Methionine 91 to Alanine, ORF1p Glu…
    Promoter
    Available since
    Nov. 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    202587-plasmid-map-sequence-id-415633

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  12. pETM11-6xHis-TEV-hORF1p_K3AK4A

    Plasmid
    #202585
    Purpose
    Allows for IPTG-inducible expression of the human L1RP ORF1 protein with the K3A and K4A mutations in bacteria with an N-terminal 6xHis tag and TEV cleavage site for purification with a minimal scar
    Depositor
    Liam Holt
    Article
    Sil et al Elife. 2023 Apr 28;12:e82991. doi: 10.75
    Insert
    ORF1p
    Use
    Tags
    6x His and TEV protease cleavage site
    Expression
    Bacterial
    Mutation
    Changed ORF1p Lysine 3 to Alanine, changed ORF1p …
    Promoter
    Available since
    Nov. 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    202585-plasmid-map-sequence-id-414431

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  14. MLKP2delKASH

    Plasmid
    #130838
    Purpose
    Expression of plant LINC fluorescent fusion protein for cell biology
    Depositor
    Hank Bass
    Article
    Gumber et al J Cell Sci. 2019 Feb 11;132(3). pii: jcs
    Insert
    MLKP2
    Use
    Tags
    eGFP-FLAG-HA
    Expression
    Plant
    Mutation
    Deletion of KASH domain, addition of three alani…
    Promoter
    CaMV 35S
    Available since
    Sept. 28, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    130838-plasmid-map-sequence-id-255274

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  16. pCAGGS-C-TEV-HAT

    Plasmid
    #202597
    Purpose
    Express N-terminal Tobacco Etch Virus (TEV) protease cleavable Histidine Affinity Tag (HAT) (KDHLIHNVHKEEHAHAHNK) in mammalian cell
    Depositor
    Chiaki Murakami
    Article
    Furuta et al FEBS Open Bio. 2023 May 11. doi: 10.1002
    Type
    Empty backbone
    Use
    Tags
    TEV cleavable site (ENLYFQG) and poly-histidine a…
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    July 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    202597-plasmid-map-sequence-id-399552

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  17. pCAGGS-N-TEV-HAT

    Plasmid
    #202598
    Purpose
    Express C-terminal Tobacco Etch Virus (TEV) protease cleavable Histidine Affinity Tag (HAT) (KDHLIHNVHKEEHAHAHNK) in mammalian cell
    Depositor
    Chiaki Murakami
    Article
    Furuta et al FEBS Open Bio. 2023 May 11. doi: 10.1002
    Type
    Empty backbone
    Use
    Tags
    TEV cleavable site (ENLYFQG) and poly-histidine a…
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    July 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    202598-plasmid-map-sequence-id-399468

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  18. pOET3-C-TEV-Twin-Strep

    Plasmid
    #203324
    Purpose
    Generate the baculovirus to express N-terminal Tobacco Etch Virus (TEV) protease cleavable Twin-Strep-tagged protein (ENLYFQGS-WSHPQFEK-(GGGS)2-GGSA-WSHPQFEK) in insect cells
    Depositor
    Chiaki Murakami
    Article
    Furuta et al FEBS Open Bio. 2023 May 11. doi: 10.1002
    Type
    Empty backbone
    Use
    Tags
    TEV cleavable site (ENLYFQG) and Twin-strep-tag
    Expression
    Insect
    Mutation
    Promoter
    Available since
    July 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    203324-plasmid-map-sequence-id-399463

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Showing: 5001 - 5020 of 14727 results