Addgene

We narrowed to 22,131 results for: Nov

Showing: 4981 - 5000 of 22131 results

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pcI-ts,ind+-(NSP8)

Plasmid

#160656

Purpose

Expresses codon optimized SARS-CoV-2 NSP8 in E. coli

Depositor

Kenneth Johnson

Article

Dangerfield et al iScience. 2020 Nov 28:101849. doi: 10.10

Insert

SARS-CoV-2 NSP8 (ORF1ab Synthetic)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 10, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pED-hIRE1(I642G)

Plasmid

#16263

Depositor

Peter Walter

Article

Lin et al Science. 2007 Nov 9. 318(5852):944-9.

Insert

IRE1 (ERN1 Human)

Use

Tags

Expression

Mammalian

Mutation

isoleucine to glycine mutation at residue 642

Promoter

Available since

Nov. 30, 2007

Availability

Academic Institutions and Nonprofits only

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EF1a_ONECUT1_P2A_Hygro_Barcode

Plasmid

#120470

Purpose

Barcoded lentiviral vector to express ONECUT1 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

ONECUT1 (ONECUT1 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

EF1a

Available since

Feb. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pMTMVi-N

Plasmid

#118756

Purpose

Intermediate plasmid to build tobacco mosaic virus recombinant clones that do not move systemically throughout the plant

Depositor

Jose-Antonio Daros

Article

Shi et al Plant Biotechnol J. 2018 Nov 22. doi: 10

Insert

Tobacco mosaic virus cDNA fragment with a cloning polylinker

Use

Intermediate to build tobacco mosaic virus recomb…

Tags

Expression

Mutation

Promoter

Available since

Feb. 20, 2019

Availability

Academic Institutions and Nonprofits only

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TRS6E1b-luc(-732/-721) WT

Plasmid

#45383

Depositor

Thomas Gelehrter

Article

Song et al J Biol Chem. 1998 Nov 6;273(45):29287-90

Insert

6 copies of hPAI-1 promoter -732/-721

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

Promoter

Available since

June 20, 2013

Availability

Academic Institutions and Nonprofits only
pZ001 (AacC2c1 locus)

Plasmid

#70166

Purpose

Expresses AacC2c1 full locus

Depositor

Feng Zhang

Article

Shmakov et al Mol Cell. 2015 Nov 5;60(3):385-97. doi:

Insert

AacC2c1 locus

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 15, 2015

Availability

Academic Institutions and Nonprofits only

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pGEX-4T-1-XPLN

Plasmid

#73375

Purpose

GST-tagged expression of XPLN (ARHGEF3) in bacteria

Depositor

Krister Wennerberg, Jie Chen

Article

Arthur et al J Biol Chem. 2002 Nov 8;277(45):42964-72

Insert

arhgef3 (ARHGEF3 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

Available since

April 21, 2016

Availability

Academic Institutions and Nonprofits only
Grp1-PH pGEX2TK

Plasmid

#71379

Purpose

bacterial expression of PH domain for phosphoinositide binding assay

Depositor

Mark Lemmon

Article

Kavran et al J Biol Chem. 1998 Nov 13;273(46):30497-5

Insert

Grp1-PH (Cyth3 Mouse)

Use

Tags

GST

Expression

Bacterial

Mutation

Pleckstrin homology (PH) domain; aa 264-391

Promoter

tac

Available since

Feb. 26, 2016

Availability

Academic Institutions and Nonprofits only
pCI HA NEDD4L S382A S468A

Plasmid

#27003

Depositor

Joan Massague

Article

Gao et al Mol Cell. 2009 Nov 13. 36(3):457-68.

Insert

NEDD4L (NEDD4L Human)

Use

Tags

HA

Expression

Mammalian

Mutation

S382A S468A

Promoter

Available since

Jan. 25, 2011

Availability

Academic Institutions and Nonprofits only
pLib6.4-Agam_695

Plasmid

#176654

Purpose

Expression of sgRNA under An. gambiae U6-2 (AGAP013695) promoter & delivery of D.mel-Actin5C::EGFP-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism

Depositor

Norbert Perrimon

Article

Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

Type

Empty backbone

Use

Crispr

Tags

Expression

Insect

Mutation

Promoter

Available since

Nov. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pLN599 (pFuGW-hUbCp-hSTE1)

Plasmid

#105206

Purpose

ubiquitin C promoter drives STE expression

Depositor

Timothy Lu

Article

Nissim et al Cell. 2017 Nov 16;171(5):1138-1150.e15.

Insert

hUbCp-hSTE1

Use

Lentiviral and Synthetic Biology

Tags

HA tag

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 20, 2021

Availability

Academic Institutions and Nonprofits only

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MSCVpic2blast-EGFP-FF2

Plasmid

#164923

Purpose

Expresses EGFP cDNA and FF2 shRNA in mammalian cells

Depositor

Ji Luo

Article

Smith et al Cell Biosci. 2016 Nov 11;6:57. doi: 10.1

Inserts

Enhanced green fluorescent protein
Neuropeptide FF receptor 2 (NPFFR2 Human)

Use

Retroviral

Tags

Expression

Mutation

Promoter

Available since

May 26, 2021

Availability

Academic Institutions and Nonprofits only

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MiniCoopR U6:gRNA, mitfa:Cas9

Plasmid

#118840

Purpose

Targets a gene and expresses zebrafish mitfa specifically in zebrafish melanocytes

Depositor

Leonard Zon

Article

Ablain et al Science. 2018 Nov 1. pii: science.aau650

Type

Empty backbone

Use

CRISPR; Tol2

Tags

Expression

Mutation

Promoter

Available since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

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pGal-BE3

Plasmid

#172409

Purpose

Yeast plasmid encoding the third-generation CRISPR base editor BE3 (Komor et al., Nature 2016) under a galactose-inducible promoter

Depositor

Leonid Kruglyak

Article

Schubert et al Elife. 2022 Nov 3;11. pii: 79525. doi: 1

Insert

BE3

Use

CRISPR

Tags

Expression

Yeast

Mutation

Promoter

GALL

Available since

Nov. 7, 2022

Availability

Academic Institutions and Nonprofits only

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CaV2.1 (pCR3.1)

Plasmid

#26573

Depositor

Diane Lipscombe

Article

Richards et al Eur J Neurosci. 2007 Nov . 26(10):2950-6

Insert

Cacna1a (Cacna1a Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 29, 2010

Availability

Academic Institutions and Nonprofits only
pGAN147

Plasmid

#129202

Purpose

yeast transcriptional reporter of aggregating proteins (yTRAP) to detect protein aggregation, Gateway backbone

Depositor

Ahmad Khalil

Article

Newby et al Cell. 2017 Nov 2;171(4):966-979.e18. doi

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

SUP35

Available since

Oct. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3 MDM2 1-400

Plasmid

#45754

Depositor

Robert Lefkowitz

Article

Shenoy et al Science. 2001 Nov 9;294(5545):1307-13. E

Insert

MDM2 1-400 (MDM2 Human)

Use

Tags

Expression

Mammalian

Mutation

aa 1-400, lacks catalytic RING domain

Promoter

CMV

Available since

Aug. 21, 2013

Availability

Academic Institutions and Nonprofits only
pLib6.4-Aaeg_774

Plasmid

#176659

Purpose

Expression of sgRNA under Ae. Aegypti U6 (AAEL017774) promoter & delivery of D.mel-Actin5C::EGFP-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism

Depositor

Norbert Perrimon

Article

Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

Type

Empty backbone

Use

Crispr

Tags

Expression

Insect

Mutation

Promoter

Available since

Dec. 3, 2021

Availability

Academic Institutions and Nonprofits only

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pBABE puro Brca1 S1423A S1524A HA

Plasmid

#41968

Depositor

Stephen Elledge

Article

Cortez et al Science. 1999 Nov 5. 286(5442):1162-6.

Insert

BRCA1 (BRCA1 Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Mutation

S1423A, S1524A

Promoter

Available since

April 26, 2013

Availability

Academic Institutions and Nonprofits only
OA-1055J

Plasmid

#200251

Purpose

pBac-U6-gRNA(Intersex)-3xp3-tdTomato

Depositor

Omar Akbari

Article

Li et al bioRxiv. 2023 Nov 15:2023.04.18.537404.

Insert

2 gRNAs targeting Intersex gene

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

May 12, 2023

Availability

Academic Institutions and Nonprofits only

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