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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 12,282 results for: LEA

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Showing: 4981 - 5000 of 12282 results

  5. pcDNA3-CBX1mut-PA-mCherry

    Plasmid
    #138251
    Purpose
    Triple-mutant CBX1 chromodomain (residues 20-73) tagged with photoactivatable mCherry (PAmCherry). Mutations V3E/K6E/D40S correspond to positions V22/K25/D59 in full-length CBX1.
    Depositor
    Jin Zhang
    Publication
    Hard et al Sci Adv. 2018 Nov 7;4(11):eaau1447. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    CBX1mut-PAmCherry
    Tags
    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    July 30, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    138251-plasmid-map-sequence-id-307435

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  6. TBXTA-c027

    Plasmid
    #139764
    Purpose
    Protein expression/Streptavidin pull-down
    Depositor
    Opher Gileadi
    Insert
    TBXT, Full-length, G177D (TBXT Human)
    Tags
    Avi-tag (Biotin) and His6-GST-TEV
    Expression
    Bacterial
    Mutation
    G177D; contains only amino acids S2- M435
    Promoter
    T7
    Available since
    May 7, 2020
    Availability
    Academic Institutions and Nonprofits only
    139764-plasmid-map-sequence-id-296577

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  7. pGBW-m4046337

    Plasmid
    #145566
    Purpose
    Yeast Expression plasmid for SARS-CoV-2 nsp4
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 nsp4 (ORF1ab Severe acute respiratory syndrome coronavirus 2)
    Tags
    Cleavable thrombin;6xHIS
    Expression
    Yeast
    Mutation
    Saccharomyces cerevisiae recode 1
    Promoter
    pGAL1
    Available since
    April 29, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    145566-plasmid-map-sequence-id-298841

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  10. JAK3 (JAK3A-c023)

    Plasmid
    #98229
    Purpose
    Baculovirus expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    JAK3A (JAK3 Human)
    Use
    Baculovirus
    Tags
    His-6-TEV
    Mutation
    pfam00069:Pkinase 822-1091
    Promoter
    polyhedrin
    Available since
    Sept. 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    98229-plasmid-map-sequence-id-193400

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Showing: 4981 - 5000 of 12282 results