We narrowed to 15,824 results for: LEA

1. Hy_pMT Flag-UAP56 WT

   Plasmid

   #110125

   Purpose

   Expresses Flag-tagged human UAP56 (DDX39B)

   Depositor

   Jeremy Wilusz

   Article

   Huang et al Genes Dev. 2018 May 17. pii: gad.314856.

   Insert

   UAP56 (DDX39B Human)
   

   Use

   Tags

   Flag

   Expression

   Insect

   Mutation

   Promoter

   Metallothionein Promoter (pMT)

   Available since

   Nov. 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3-Nuc-ExRai-AMPKAR

   Plasmid

   #192450

   Purpose

   ExRai-AMPKAR targeted to the nucleus.

   Depositor

   Jin Zhang

   Article

   Schmitt et al Nat Commun. 2022 Jul 5;13(1):3856. doi:

   Insert

   ExRai-AMPKAR-NLS
   

   Use

   Tags

   6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pWay21-MKP-1 FL

   Plasmid

   #13469

   Depositor

   Anton Bennett

   Article

   Wu et al Mol Cell Biol. 2005 Jun . 25(11):4792-80

   Insert

   MKP-1 FL (Dusp1 Mouse)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 25, 2007

   Availability

   Academic Institutions and Nonprofits only

4. pET21-pelB-LaM-2

   Plasmid

   #172768

   Purpose

   Bacterial expression of anti-mCherry nanobody LaM-2, with pelB leader and C-term free cysteine and 6xHIS tag.

   Depositor

   Michael Rout

   Article

   Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

   Insert

   LaM-2
   

   Use

   Tags

   6xHIS and pelB leader for periplasmic secretion

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pICSL12009

   Plasmid

   #68257

   Purpose

   Level 0 Golden Gate Part: Promoter and 5UTR Ubiquitin (Zea mays)

   Depositor

   Nicola Patron

   Article

   Lawrenson et al Genome Biol. 2015 Nov 30;16(1):258. doi:

   Insert

   Promoter and 5UTR Ubiquitin (Zea mays)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 20, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCL1271

   Plasmid

   #217963

   Purpose

   Mammalian expression of Importin 5 (Imp5, IPO5) with an N-terminal eGFP fusion. Splice isoform 1.

   Depositor

   Andrew Bowman

   Article

   Apta-Smith et al EMBO J. 2018 Oct 1;37(19):e98714. doi: 1

   Insert

   Imp5 (IPO5 Human)
   

   Use

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. Gal4-PGC1 alpha

   Plasmid

   #8892

   Depositor

   Bruce Spiegelman

   Article

   Puigserver et al Cell 1998 Mar 20;92(6):829-39.

   Insert

   PGC-1 alpha (Ppargc1a Mouse)
   

   Use

   Tags

   Gal4 DBD

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 19, 2006

   Availability

   Academic Institutions and Nonprofits only

8. pRSFDuet-1_VNp-Uricase

   Plasmid

   #182401

   Purpose

   Bacterial expression of Vesicle Nucleating peptide-uricase fusion

   Depositor

   Dan Mulvihill

   Article

   Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

   Insert

   VNp-uricase
   

   Use

   Tags

   Vesicle Nucleating peptide (VNp)

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Feb. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. MSCV-mNINJ1-K45Q-GFP

   Plasmid

   #208777

   Purpose

   MSCV vector expressing K45Q mouse NINJ1

   Depositor

   Petr Broz

   Article

   Ramos et al EMBO J. 2024 Apr;43(7):1164-1186. doi: 1

   Insert

   NINJ1 (Ninj1 Mouse)
   

   Use

   Retroviral

   Tags

   GFP

   Expression

   Mutation

   Change Lys 45 to Gln (K45Q)

   Promoter

   Available since

   Aug. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-Dlx-FLEX-hM4Di-mCherry

    Plasmid

    #209701

    Purpose

    Expresses hM4Di-mCherry under the control of distalless homeobox 5 and 6 (mDlx) enhancer in Cre-recombinase-dependent manner

    Depositor

    Peer Wulff

    Article

    Rangel Guerrero et al Neuron. 2024 Apr 10:S0896-6273(24)00197-

    Insert

    hM4D(Gi)
    

    Use

    AAV

    Tags

    mCherry

    Expression

    Mutation

    Promoter

    Available since

    April 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. OVA-miSFIT-17-T

    Plasmid

    #124677

    Purpose

    Tuning expression of cytosolic Ovalbumin coupled to EGFP

    Depositor

    Tudor Fulga

    Article

    Michaels et al Nat Commun. 2019 Feb 18;10(1):818. doi:

    Insert

    Cyto-Ovalbumin
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Truncated OVA for cytoplasmic expression

    Promoter

    minimal CMV

    Available since

    May 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pETM11-6xHis-TEV-hORF1p_K3AK4A

    Plasmid

    #202585

    Purpose

    Allows for IPTG-inducible expression of the human L1RP ORF1 protein with the K3A and K4A mutations in bacteria with an N-terminal 6xHis tag and TEV cleavage site for purification with a minimal scar

    Depositor

    Liam Holt

    Article

    Sil et al Elife. 2023 Apr 28;12:e82991. doi: 10.75

    Insert

    ORF1p
    

    Use

    Tags

    6x His and TEV protease cleavage site

    Expression

    Bacterial

    Mutation

    Changed ORF1p Lysine 3 to Alanine, changed ORF1p …

    Promoter

    Available since

    Nov. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCI syn iGluSnFR

    Plasmid

    #106123

    Purpose

    Indicator for glutamate imaging (synapsin promoter)

    Depositor

    Katalin Torok

    Article

    Helassa et al Proc Natl Acad Sci U S A. 2018 May 7. pi

    Insert

    iGluSnFR
    

    Use

    Tags

    Myc and PDGFR

    Expression

    Mammalian

    Mutation

    Promoter

    synapsin

    Available since

    June 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. 3521 pET15b Bid p15

    Plasmid

    #8782

    Depositor

    Stanley Korsmeyer

    Article

    Wei et al Genes Dev 2000 Aug 15;14(16):2060-71.

    Insert

    Bid p15 (Bid Mouse)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 6, 2005

    Availability

    Academic Institutions and Nonprofits only

15. pYPQ230-E795L

    Plasmid

    #176890

    Purpose

    Gateway entry plasmid (attL1 & attR5) expressing LbCas12a with E795L mutation, without promoter

    Depositor

    Yiping Qi

    Article

    Zhang et al Front Genome Ed. 2022 Jan 31;4:780238. d

    Insert

    LbCas12a-E795L
    

    Use

    CRISPR; Gateway compatible lbcpf1 entry clone

    Tags

    Nucleoplasmin NLS and SV40 NLS

    Expression

    Plant

    Mutation

    Promoter

    Available since

    April 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. WT (REP10)

    Plasmid

    #21368

    Depositor

    Gregory Germino

    Article

    Qian et al Proc Natl Acad Sci U S A. 2002 Dec 24. 9

    Insert

    autosomal dominant polycystic kidney disease type I (PKD1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 4, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3-ABKAR

    Plasmid

    #140051

    Purpose

    Genetically encoded FRET-based biosensor for monitoring AMPK activity. Also sensitive to phosphorylation by BRSK1/2 when expressed in neurons.

    Depositor

    Jin Zhang

    Article

    Sample et al Mol Biol Cell. 2015 May 15;26(10):1935-4

    Insert

    ABKAR
    

    Use

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. 3xflag-APEX2-NES

    Plasmid

    #164623

    Purpose

    Expresses a nuclear exporting signal (NES) tagged with APEX2 and 3xflag

    Depositor

    Mark Cookson

    Article

    Bonet-Ponce et al Sci Adv. 2020 Nov 11;6(46). pii: 6/46/ea

    Insert

    NES
    

    Use

    Tags

    3xFLAG and APEX2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. mRFP-LAP2β

    Plasmid

    #228029

    Purpose

    lentiviral construct for mRFP-LAP2β expression

    Depositor

    David Pellman

    Article

    Zhao et al Dev Cell. 2023 May 22;58(10):847-865.e10

    Insert

    LAP2b (Tmpo Rat)
    

    Use

    Lentiviral

    Tags

    mRFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pTMEM-PhoCl-Insert

    Plasmid

    #219674

    Purpose

    Caging at cytosolic N-ter of POI: TMEM-PhoCl-rtta3mScarlet

    Depositor

    Helge Ewers

    Article

    Kashyap et al Nat Methods. 2024 Mar 8. doi: 10.1038/s4

    Insert

    TMEM-PhoCl-rtTA3
    

    Use

    Tags

    PhoCl

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 1, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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