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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 23,536 results for: LEA

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Showing: 4981 - 5000 of 23536 results

  4. pcDNA3-RAB-EKARev(T/A)

    Plasmid
    #118475
    Purpose
    Negative-control mutant for RAB-EKARev biosensor.
    Depositor
    Jin Zhang
    Publication
    Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    RAB-EKARev(T/A)
    Tags
    6xHIS, T7 tag (gene 10 leader), and Xpress (TM) t…
    Expression
    Mammalian
    Mutation
    Contains Thr-to-Ala mutation in substrate sequenc…
    Promoter
    CMV
    Available since
    May 21, 2019
    Availability
    Academic Institutions and Nonprofits only
    118475-plasmid-map-sequence-id-243129

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  5. pCMV6-G1-ATP6-Myc-FLAG (puromycin)

    Plasmid
    #86851
    Purpose
    mammalian expression of ATP6 with mitochondrial targeting sequence, Myc, and FLAG tags
    Depositor
    Matthew O'Connor
    Publication
    Boominathan et al Nucleic Acids Res. 2016 Nov 2;44(19):934
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ATP6 (ATP6 Human)
    Tags
    ATP5G1 (G1) (MQTAGALFISPALIRCCTRGLIRPVSASFLNS PVN…
    Expression
    Mammalian
    Mutation
    codon corrected
    Promoter
    CMV
    Available since
    May 31, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    86851-plasmid-map-sequence-id-488638

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  8. pcDNA3-ICUE3-NLS

    Plasmid
    #138218
    Purpose
    Nuclear-targeted FRET-based cAMP indicator.
    Depositor
    Jin Zhang
    Publication
    Sample et al Nat Chem Biol. 2012 Feb 26;8(4):375-82.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ICUE3-NLS
    Tags
    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Feb. 5, 2021
    Availability
    Academic Institutions and Nonprofits only
    138218-plasmid-map-sequence-id-307457

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  9. pSH-Csy4-T2A-SpRFN

    Plasmid
    #85754
    Purpose
    Expresses S. pyogenes FokI-dCas9-NLS with a 25 amino acid linker (GGGGS)5 fusion. Also expresses Csy4 for cleavage of multiplexed gRNA transcripts. Derived from pSQT1601 (Addgene #53369).
    Depositor
    Lawrence Stanton
    Publication
    Havlicek et al Mol Ther. 2017 Feb 1;25(2):342-355. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Csy4-T2A-FokI-dCas9
    Use
    CRISPR
    Tags
    Csy4 and FokI fusion via 25 amino acid (GGGGS)5 l…
    Expression
    Mammalian
    Mutation
    D10A, H840A
    Promoter
    CAG
    Available since
    Jan. 20, 2017
    Availability
    Academic Institutions and Nonprofits only
    85754-plasmid-map-sequence-id-170272

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  10. p2B-55

    Plasmid
    #177924
    Purpose
    Expresses optimized unleaky reverse tetracycline transactivator (rtTA-M2-SE-G72P) under control of yeast TDH3 promoter
    Depositor
    Vadim Gladyshev
    Publication
    Barre et al (unpublished)
    Insert
    Optimized unleaky reverse tetracycline transactivator (rtTA-M2-SE-G72P) under control of yeast TDH3 promoter
    Expression
    Bacterial and Yeast
    Mutation
    V9I; F67S; G72P, F86Y; R171K
    Promoter
    TDH3
    Available since
    Dec. 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    177924-plasmid-map-sequence-id-349994

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  18. pCDF-5xT7-TtCsm

    Plasmid
    #128572
    Purpose
    For expression of T. thermophilus Csm in E. coli (Csm1-5)
    Depositor
    Jennifer Doudna
    Publication
    Liu et al Nat Commun. 2019 Jul 5;10(1):3001. doi:
    Inserts
    Csm1
    Csm2, Csm3, Csm4, Csm5
    Tags
    HRV 3C protease cleavage site and 10xHis tag on C…
    Expression
    Bacterial
    Mutation
    Codon optimized for expression in E. coli and Cod…
    Promoter
    T7 promoter, lac operator
    Available since
    Aug. 6, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    128572-plasmid-map-sequence-id-252577

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Showing: 4981 - 5000 of 23536 results