We narrowed to 15,542 results for: LEA

1. pTRE2-Bla(HA–RILP-FLAG)

   Plasmid

   #102424

   Purpose

   Dual tagged RILP for expression in mammalian cells.

   Depositor

   Steven Weinman

   Article

   Wozniak et al Proc Natl Acad Sci U S A. 2016 Nov 1;113

   Insert

   Rab interacting lysosomal protein (RILP Human)
   

   Use

   Tags

   FLAG and HA

   Expression

   Mammalian

   Mutation

   Promoter

   Tet-Responsive

   Available since

   Oct. 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEn-C1.1

   Plasmid

   #61479

   Purpose

   Encodes CRISPR sgRNA for Gateway or conventional cloning of 1-3 sgRNAs into pDe-CAS9 or pCAS9-TPC

   Depositor

   Holger Puchta

   Article

   Schiml et al Plant J. 2014 Oct 18. doi: 10.1111/tpj.1

   Insert

   AtU6-26:sgRNA
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   U6-26

   Available since

   Sept. 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDARMO_CMVT_N_Strep_BIRC6

   Plasmid

   #197972

   Purpose

   Mammalian cell expression of wt Strep-BIRC6

   Depositor

   Eric Fischer

   Article

   Hunkeler et al Science. 2023 Mar 17;379(6637):1105-1111

   Insert

   BIRC6 (BIRC6 Human)
   

   Use

   Tags

   Strep

   Expression

   Mammalian

   Mutation

   L1332V

   Promoter

   Available since

   April 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. Pet15b-Endosomal Escape Control

   Plasmid

   #186552

   Purpose

   Negative Control confirming the need for a CPP peptide in mediating internalization

   Depositor

   Jean Gariepy

   Article

   Mohammed et al J Control Release. 2012 Nov 28;164(1):58

   Insert

   6H-ETA-GFP
   

   Use

   Tags

   6xHis and EGFP

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. Pet15b-Internalization and Endosomal Escape

   Plasmid

   #186551

   Purpose

   Internalization and Endosomal Escape to effectively route a protein cargo to the cytosol of cells

   Depositor

   Jean Gariepy

   Article

   Mohammed et al J Control Release. 2012 Nov 28;164(1):58

   Insert

   10R-8His-ETA-EGFP
   

   Use

   Tags

   8xHis and EGFP

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pASPIre2

   Plasmid

   #154843

   Purpose

   Derivative of pASPIre1 carrying a bxb1-sfGFP translational fusion in place of bxb1

   Depositor

   Markus Jeschek

   Article

   Hollerer et al Nat Commun. 2020 Jul 15;11(1):3551. doi:

   Insert

   Bxb1-sfGFP fusion controlled by rhamnose-inducible promoter; attB/attP-flanked discriminator containing mCherry; rhaRS operon
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAdSh.U6.gRNAGFP

   Plasmid

   #58255

   Purpose

   U6 promoter-driven single guide RNA complementary to eGFP

   Depositor

   Manuel Goncalves

   Article

   Maggio et al Sci Rep. 2014 May 29;4:5105. doi: 10.103

   Insert

   U6.gRNAGFP cassette
   

   Use

   Adenoviral and CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 5, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pTSSX-STC

   Plasmid

   #115668

   Purpose

   E.coli expression plasmid of small tetraheme cytochrome (STC) from Shewanella oneidensis MR-1 with cleavable Strep tag

   Depositor

   David Kramer

   Article

   Huang et al J Am Chem Soc. 2020 Jun 10;142(23):10459

   Insert

   small tetraheme cytochrome c
   

   Use

   Tags

   Signal peptide (aa1-27)-Strep-II tag- Factor Xa c…

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available since

   Oct. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. JW617

   Plasmid

   #64809

   Purpose

   CUC3 expression in yeast

   Depositor

   Detlef Weigel

   Article

   Rubio-Somoza et al Curr Biol. 2014 Nov 17;24(22):2714-9. do

   Insert

   CUC3 (CUC3 Mustard Weed)
   

   Use

   Tags

   GAL4 DNA binding domain and Myc

   Expression

   Mutation

   Promoter

   Available since

   Aug. 26, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBT312_pH11-U-attP-FRT5-pSV40-Neo-pA-pVasa-PhiC31o-pA-FRT5

    Plasmid

    #52545

    Purpose

    Targeting vector for homologous recombination in mouse ES cells; Hipp11 locus

    Depositor

    Liqun Luo

    Article

    Tasic et al Proc Natl Acad Sci U S A. 2011 May 10;10

    Inserts

    PhiC31o attP (full length)
    Neo
    PhiC31o integrase
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEN-X-hPGK-PuroR-Z

    Plasmid

    #218904

    Purpose

    golden gate cloning

    Depositor

    Sven Eyckerman

    Article

    Delhaye et al Cell Rep Methods. 2024 Jul 15;4(7):10081

    Insert

    hPGK-PuroR
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    246T>A and 398T>C

    Promoter

    Available since

    May 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. JW624

    Plasmid

    #64808

    Purpose

    CUC2 expression in yeast

    Depositor

    Detlef Weigel

    Article

    Rubio-Somoza et al Curr Biol. 2014 Nov 17;24(22):2714-9. do

    Insert

    CUC2 (CUC2 Mustard Weed)
    

    Use

    Tags

    GAL4 DNA binding domain

    Expression

    Mutation

    Promoter

    Available since

    Sept. 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pOCC102

    Plasmid

    #118883

    Purpose

    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6-MBP tag, cleavable with 3C protease

    Depositor

    Marc Bickle, PEPC MPI CBG

    Article

    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

    Insert

    NcoI-HIS6-MBP-3C-NotI-ccdB-AscI-stop-HindIII cassette
    

    Use

    Tags

    HIS6-MBP, cleavable with 3C protease

    Expression

    Insect

    Mutation

    Promoter

    polH

    Available since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET21-pelB-LaG-11

    Plasmid

    #172762

    Purpose

    Bacterial expression of HIS-tagged anti-GFP nanobody LaG-11; HIS tag preceded by HRV 3C/PreScission cleavage site.

    Depositor

    Michael Rout

    Article

    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

    Insert

    LaG-11
    

    Use

    Tags

    6xHIS and pelB leader for periplasmic secretion

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET21-pelB-LaM-1

    Plasmid

    #172767

    Purpose

    Bacterial expression of anti-mCherry nanobody LaM-1, with pelB leader and C-term free cysteine and 6xHIS tag.

    Depositor

    Michael Rout

    Article

    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

    Insert

    LaM-1
    

    Use

    Tags

    6xHIS and pelB leader for periplasmic secretion

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. Hyper7 in pT3TS-Dest

    Plasmid

    #194296

    Purpose

    In vitro transcription of the hydrogen peroxide sensor Hyper7 from the T3 promoter. Parton lab clone KRL

    Depositor

    Rob Parton

    Article

    Wu et al Dev Cell. 2023 Mar 13;58(5):376-397.e4.

    Insert

    Hyper7
    

    Use

    In vitro transcription of mrna

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pY018 (pcDNA3.1-hPdCpf1)

    Plasmid

    #69990

    Purpose

    Expresses humanized PdCpf1

    Depositor

    Feng Zhang

    Article

    Zetsche et al Cell. 2015 Sep 23. pii: S0092-8674(15)01

    Insert

    hPdCpf1
    

    Use

    CRISPR

    Tags

    NLS & 3xHA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. cycLuc_PPVs

    Plasmid

    #119208

    Purpose

    Circularly permutated firefly luciferase with PPVs cleavage site (Luciferase reporter for detection of proteolytic activity)

    Depositor

    Roman Jerala

    Article

    Fink et al Nat Chem Biol. 2019 Feb;15(2):115-122. d

    Insert

    cyclic luciferase with PPVp cleavage site
    

    Use

    Luciferase

    Tags

    AU1 tag

    Expression

    Mutation

    Promoter

    CMV

    Available since

    June 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET41a iGluh

    Plasmid

    #119830

    Purpose

    Production of recombinant iGluSnFR E25A high affinity variant, a single-wavelength glutamate indicator, in E. coli

    Depositor

    Katalin Torok

    Article

    Helassa et al Proc Natl Acad Sci U S A. 2018 May 7. pi

    Insert

    iGluh
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    E25A

    Promoter

    T7

    Available since

    Aug. 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV-Nd1

    Plasmid

    #191523

    Purpose

    Expression of mitochondrial v8(HS1) ZF-DdCBE pair in mammalian cells (LB510-Nd1+RB54-Nd1); AAV packaging vector

    Depositor

    David Liu

    Article

    Willis et al Nat Commun. 2022 Nov 23;13(1):7204. doi:

    Insert

    v8(HS1) ZF-DdCBE pair LB510-Nd1+RB54-Nd1 AGKS
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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