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Plasmid#106367PurposeC. difficile inducible expression system. Shuttle plasmid containing a tetracycline-inducible gusADepositorInsertTerm(fdx)-Ptet-gusA-Term(slpA) (gusA )
UseE. coli - c. difficile shuttle vectorTagsExpressionMutationcodon optimised for C. difficilePromoterPtetAvailable sinceMarch 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
A2_uSFFV
Plasmid#153415PurposeExpresses HLA-A*02:01 MHCI gene by the UCOE-SFFV promoterDepositorInsertA*02:01-P2A-human beta2 microglobulin (B2M Human)
UseLentiviralTagsExpressionMammalianMutationPromoterSFFVAvailable sinceJuly 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-ARAP3
Plasmid#39484DepositorInsertARAP3 (ARAP3 Human)
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceAug. 22, 2012AvailabilityAcademic Institutions and Nonprofits only -
pMH610
Plasmid#131862PurposeProduces holo-PhyB* in E. coliDepositorInsertsPhyB(1-651)-RGD-His6-Cys
HO1
PcyA
UseTagsExpressionBacterialMutationPromoterT7 promoter-1 and T7 promoter-2Available sinceOct. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pU6-BbsI-chiRNA
Plasmid#45946PurposePlasmid for expression of chiRNA under the control of the Drosophila snRNA:U6:96Ab promoter.DepositorInsertU6-BbsI-chiRNA
UseCRISPRTagsExpressionInsectMutationPromoterDm-snRNA:U6:96AbAvailable sinceJune 24, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAAV hSyn FLEx mGFP-2A-Synaptophysin-mRuby
Plasmid#71760PurposemGFP and Synaptophysin-mRuby expression from Cre-expressing neuronsDepositorHas ServiceAAV1InsertsmGFP
Synaptophysin
UseAAV and Cre/LoxTagsPalmitoylation signal and mRubyExpressionMutationPromoterAvailable sinceDec. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
Lenti-luciferase-P2A-Neo
Plasmid#105621Purpose3rd generation lentiviral plasmid for expression of luciferase with Neo resistanceDepositorInsertLuciferase
UseLentiviral and LuciferaseTagsExpressionMammalianMutationPromoterEFSAvailable sinceFeb. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJ251-GERC
Plasmid#47441PurposeExpresses mCherry and sfGFP from divergent promoters. The backbone is cloned into DNA2.0 pJ251, and the design is based off of pZS2-123.DepositorInsertGene Expression Reporter Construct
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 27, 2013AvailabilityAcademic Institutions and Nonprofits only -
pTRE-tight2-Tmem98-FLAG
Plasmid#124506PurposeExpresses FLAG-tagged mouse TMEM98 in mammalian cells in a doxycycline-dependent manner (C-terminal tag)DepositorInsertTmem98 (Tmem98 Mouse)
UseTagsFlagExpressionMammalianMutationPromotertetO-CMVAvailable sinceMay 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CMV-Cas9C-VPR
Plasmid#80933PurposeExpresses Cas9C-VPR in mammalian cells.DepositorInsertCas9C-VPR
UseAAV, CRISPR, and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceOct. 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
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pDONR223-PLK1
Plasmid#23867DepositorInsertPLK1 (PLK1 Human)
UseGateway donor vectorTagsExpressionMutationA3PPromoterAvailable sinceJuly 30, 2010AvailabilityAcademic Institutions and Nonprofits only -
AAV-DeltaJunD
Plasmid#68547PurposeAAV expression of DeltaJunDDepositorInsertDeltaJunD-IRES-GFP
UseAAVTagsExpressionMammalianMutationtruncated splice variantPromoterCMVAvailable sinceJuly 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGAPZa-STE2-1D4
Plasmid#133422PurposeMutant form of yeast STE2 coding sequence in vector for protein expression in the methanogenic yeast Pichia pastoris, with GAP promoter.DepositorInsertSTE2 (STE2 Budding Yeast)
UseTagsExpressionYeastMutationC59S, C252APromoterAvailable sinceJan. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
lenti-sgRNA puro
Plasmid#104990PurposeThis 3rd generation lentiviral plasmid expresses a S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from a U6 promoter and puromycin resistance from an EF-1a promoter.DepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDsRed Monomer C1-YAP2-S127A mutant
Plasmid#19058DepositorInsertYes-kinase associated protein (YAP1 Human)
UseTagsRed-FPPExpressionMammalianMutationSerine 127 changed to AlaninePromoterAvailable sinceSept. 8, 2008AvailabilityAcademic Institutions and Nonprofits only -
Keratin 18 (pcDNA3)
Plasmid#18064DepositorInsertKeratin 18 (KRT18 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 16, 2008AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_PAsfCherry2(1-10)
Plasmid#82612PurposeExpress PAsfCherry2(1-10) in mammalian cellsDepositorInsertPAsfCherry2(1-10)
UseTagsExpressionMammalianMutationchanged Glu 118 to Gln, changed Thr 128 to IlePromoterCMVAvailable sinceJan. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLX304 CMV HA-HaloTag-FRB-sTurboID (C)
Plasmid#153003PurposeExpresses low-affinity split-TurboID C-terminal fragment in mammalian cellsDepositorInsertsTurboID (C) (G74)
UseLentiviralTagsFRB, HA, and HaloTagExpressionMammalianMutationPromoterCMVAvailable sinceJune 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Neo-Bam
Plasmid#16440PurposeContains two independent transcription units--a CMV promoter/enhancer upstream of a MCS and a HSV thymidine kinase promoter/enhancer upstream of the neomycin resistance gene.DepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceApril 7, 2008AvailabilityAcademic Institutions and Nonprofits only