Addgene

We narrowed to 11,264 results for: shRNA

Showing: 481 - 500 of 11264 results

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AAV-actin prom-dsRed-scramble shRNA

Plasmid

#67879

Purpose

AAV vector to express dsRed sh RNA (scramble)

Depositor

William Wisden

Article

Zhang et al Nat Neurosci. 2015 Apr;18(4):553-61. doi

Insert

actin prom-dsRed-scramble shRNA

Use

AAV and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

chicken beta actin

Available since

Aug. 10, 2015

Availability

Academic Institutions and Nonprofits only
AAV-phsyn-FLEX-KASH-EGFP-U6-shRNA

Plasmid

#129708

Purpose

Express cre-dependent KASH-EGFP and scramble shRNA

Depositor

Yi Zhang

Article

Tuesta et al Nat Commun. 2019 Jun 7;10(1):2508. doi:

Insert

KASH-EGFP

Use

AAV

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

U6

Available since

Aug. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pSUPER-retro-puro-tetO-CTR1-shRNA

Plasmid

#53180

Purpose

Expresses shRNA against human CTR1 from puromycin resistance retroviral vector

Depositor

Christopher Counter

Article

Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature1

Insert

CTR1 (SLC31A1 Human)

Use

RNAi and Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 19, 2014

Availability

Academic Institutions and Nonprofits only
pBS/U6/RNAi gamma-tubulin shRNA mouse

Plasmid

#70666

Purpose

Reduced the expression of gamma-tubulin in murine cells

Depositor

Maria Alvarado-Kristensson

Article

Hoog et al FASEB J. 2011 Nov;25(11):3815-27. doi: 1

Insert

5´-GGCTCATGATGGCCAACCACCAAGCTTGGTGGTTGGCCATCATGAGCCCTTTTTC-3´ 5´-CCGAGTACTACCGGTTGGTGGTTCGAACCACCAACCGGTAGTACTCGGGAAAAAGTTAA-3´ (Tubg1, Tubg2 Mouse)

Use

U6 promoter

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Dec. 17, 2015

Availability

Academic Institutions and Nonprofits only

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PLL5.0-Anillin ShRNA-Cherry-AnillinA740D, E758K

Plasmid

#187273

Purpose

Lentiviral expression of shRNA targeting Anillin + reconstitution of Anillin A740D,E758K

Depositor

Alpha Yap

Article

Budnar et al Dev Cell. 2019 Jun 17;49(6):894-906.e12.

Insert

Anillin ,hsAnillin, Entrez Gene ANLN (a.k.a. FSGS8, Scraps, scra) (ANLN Human)

Use

Lentiviral

Tags

mCherry

Expression

Mutation

Anillin A740D, E758K

Promoter

U6, CMV

Available since

Sept. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pSUPER-retro-puro-tetO-Mek1-shRNA

Plasmid

#53179

Purpose

Expresses shRNA against mouse Mek1 from puromycin resistance retroviral vector

Depositor

Christopher Counter

Article

Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature1

Insert

MEK1 (Map2k1 Mouse)

Use

RNAi and Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 19, 2014

Availability

Academic Institutions and Nonprofits only
pLVX-shRNA2-zsGreen-PGK-puro_HSALNG0104472-163

Plasmid

#197902

Purpose

For the stable knockdown experiments using shRNA, a complementary sequence (HSALNG0104472-163) targeting the HSALNG0104472 transcript was inserted.

Depositor

Bo Wang

Article

Lu et al Commun Biol. 2023 Feb 17;6(1):189. doi:

Insert

HSALNG0104472-163 (HSALNG0104472-scramble-sequence)

Use

Lentiviral

Tags

zsGreen1

Expression

Mammalian

Mutation

15q11.2 deletion

Promoter

Available since

Nov. 19, 2024

Availability

Academic Institutions and Nonprofits only

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SVA-lncRNA AK057321 shRNA 1A scramble control

Plasmid

#202835

Purpose

Lentiviral vector that expresses a scrambled control shRNA for SVA-lncRNA AK057321 shRNA 1A

Depositor

Matthew Anderson

Article

Nadler et al Commun Biol. 2023 Mar 30;6(1):347. doi:

Insert

pLVX-SVA-lncRNA shRNA 1A scramble control

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

July 6, 2023

Availability

Academic Institutions and Nonprofits only

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pCIneo-V5-SBP-MBP-HsNot9-V181E-shRNA2res_AG

Plasmid

#148829

Purpose

Mammalian Expression of HsNot9-V181E-shRNA2res

Depositor

Elisa Izaurralde

Article

Sgromo et al RNA. 2018 Mar;24(3):381-395. doi: 10.126

Insert

HsNot9-V181E-shRNA2res (CNOT9 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pCIneo-lambdaN-HA-HsSmg7-G100E-siRNAres-shRNAres_S

Plasmid

#147461

Purpose

Mammalian Expression of HsSmg7-G100E-siRNAres-shRNAres

Depositor

Elisa Izaurralde

Article

Jonas et al Genes Dev. 2013 Jan 15;27(2):211-25. doi

Insert

HsSmg7-G100E-siRNAres-shRNAres (SMG7 Human)

Use

Tags

Expression

Mammalian

Mutation

three silent mutation G216A, T1185C and A1521G an…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pCIneo-lambdaN-HA-HsSmg7-K66ER163E-siRNAres-shRNAres_S

Plasmid

#147462

Purpose

Mammalian Expression of HsSmg7-K66ER163E-siRNAres-shRNAres

Depositor

Elisa Izaurralde

Article

Jonas et al Genes Dev. 2013 Jan 15;27(2):211-25. doi

Insert

HsSmg7-K66ER163E-siRNAres-shRNAres (SMG7 Human)

Use

Tags

Expression

Mammalian

Mutation

three silent mutation G216A, T1185C and A1521G an…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pLNHA-C1-HsNot1iso1-del1097-1110-R1138A-shRNAres_W

Plasmid

#147881

Purpose

Mammalian Expression of HsNot1iso1-del1097-1110-R1138A-shRNAres

Depositor

Elisa Izaurralde

Article

Chen et al Mol Cell. 2014 Jun 5;54(5):737-50. doi:

Insert

HsNot1iso1-del1097-1110-R1138A-shRNAres (CNOT1 Human)

Use

Tags

Expression

Mammalian

Mutation

1 silent mutation compared to the sequence of iso…

Promoter

Available since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsNot1iso1_1-1605-IKGRQtoDSYAA-shRNAres_W

Plasmid

#147888

Purpose

Mammalian Expression of HsNot1iso1_1-1605-IKGRQtoDSYAA-shRNAres

Depositor

Elisa Izaurralde

Article

Chen et al Mol Cell. 2014 Jun 5;54(5):737-50. doi:

Insert

HsNot1iso1_1-1605-IKGRQtoDSYAA-shRNAres (CNOT1 Human)

Use

Tags

Expression

Mammalian

Mutation

1 silent mutation compared to the sequence of iso…

Promoter

Available since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsNot1iso1_1-1840-IKGRQtoDSYAA-shRNAres_W

Plasmid

#147890

Purpose

Mammalian Expression of HsNot1iso1_1-1840-IKGRQtoDSYAA-shRNAres

Depositor

Elisa Izaurralde

Article

Chen et al Mol Cell. 2014 Jun 5;54(5):737-50. doi:

Insert

HsNot1iso1_1-1840-IKGRQtoDSYAA-shRNAres (CNOT1 Human)

Use

Tags

Expression

Mammalian

Mutation

1 silent mutation compared to the sequence of iso…

Promoter

Available since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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PLL5.0-Anillin ShRNA-GFP-AnillinA740D, E758K

Plasmid

#187274

Purpose

Lentiviral expression of shRNA targeting Anillin + reconstitution of Anillin A740D,E758K

Depositor

Alpha Yap

Article

Budnar et al Dev Cell. 2019 Jun 17;49(6):894-906.e12.

Insert

Anillin ,hsAnillin, Entrez Gene ANLN (a.k.a. FSGS8, Scraps, scra) (ANLN Human)

Use

Lentiviral

Tags

EGFP

Expression

Mutation

Anillin A740D, E758K

Promoter

U6, 5'LTR

Available since

Sept. 26, 2022

Availability

Academic Institutions and Nonprofits only

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Lentiviral human Citron shRNA 1 RFP i670

Plasmid

#155345

Purpose

Lentiviral expression of humnan CIT shRNA, RFP i670 expression, based on Addgene 12247

Depositor

Martin Carroll

Insert

Citron (CIT Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pRFP shRNA-resistant TrkB F616A -S478A

Plasmid

#72718

Purpose

Mammalian expression of TrkB fused to RFP in C-terminus, shRNA resistant and lacking TIAM1-binding site

Depositor

Freddy Jeanneteau

Article

Arango-Lievano et al Proc Natl Acad Sci U S A. 2015 Nov 16. p

Insert

TrkB

Use

Tags

RFP

Expression

Mammalian

Mutation

shRNA-resistant TrkB (T300C, T301A, C302G, C306A,…

Promoter

CMV

Available since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only
pAAV CMV-DIO-(mCherry-U6)-shRNA(anti-Crh)

Plasmid

#214732

Purpose

Expresses shRNA against CRH, marked with mCherry, in infected and cre positive cells

Depositor

Félix Leroy

Article

de Leon Reyes et al Cell. 2023 Sep 14;186(19):4152-4171.e31.

Insert

anti-Crh shRNA / mCherry

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

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AAV-actin prom-dsRed-adra2a.1227.shRNA

Plasmid

#67880

Purpose

expresses dsRed and shRNA to knock down adra2a receptors

Depositor

William Wisden

Article

Zhang et al Nat Neurosci. 2015 Apr;18(4):553-61. doi

Insert

dsRed and shRNA to knock down the mouse adra2 receptor

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

chicken beta actin

Available since

Sept. 11, 2015

Availability

Academic Institutions and Nonprofits only
pcDNA4 h-Stx3 resistant to shRNA #304-2xMyc/His

Plasmid

#99746

Purpose

Expresses human Stx3 with a C-term myc-myc-his that is resistant to shRNA #304 from Sigma Aldrich.

Depositor

Thomas Weimbs

Article

Giovannone et al Mol Biol Cell. 2017 Oct 15;28(21):2843-2

Insert

Stx3 (STX3 Human)

Use

Tags

myc-myc-his

Expression

Mammalian

Mutation

Made six (6) silent mutations to confer resistanc…

Promoter

CMV/TO

Available since

April 17, 2018

Availability

Academic Institutions and Nonprofits only

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