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Plasmid#111513PurposeExpresses M. tuberculosis Trigger Factor with a C-terminal His tagDepositorInsertTrigger Factor
UseTagsHis tagExpressionBacterialMutationPromoterT7Available sinceJune 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a-HA-CPDSalI
Plasmid#38254DepositorTypeEmpty backboneUseTags6xHis, HA, and Vibrio cholerae MARTX toxin cystei…ExpressionBacterialMutationPromoterT7Available sinceAug. 2, 2012AvailabilityAcademic Institutions and Nonprofits only -
pET28a-UVRAG CC
Plasmid#24400DepositorInsertUVRAG (UVRAG Human)
UseTagsHisExpressionBacterialMutationContains on the CC domain, amino acids 200-270PromoterAvailable sinceApril 30, 2010AvailabilityAcademic Institutions and Nonprofits only -
pET21-MRPP1_native+
Plasmid#67865Purposebacterial expression of TRMT10C, full coding sequence (mitoch. form)DepositorInsertMRPP1 (TRMT10C Human)
UseTagsExpressionBacterialMutationmitoch. form (contains aa 40-403)PromoterT7Available sinceAug. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET21-tVYN-Nt∆
Plasmid#154431PurposePlasmid used to express the tVYN-Nt∆ luminescent biosensor for use in live cell cyclic di-GMP detectionDepositorInsertVenusΔC12-NtYcgRΔ-NLucΔ4
UseTagsExpressionBacterialMutationPromoterT7Available sinceAug. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
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pET22b-HA-CPDSalI
Plasmid#38253DepositorTypeEmpty backboneUseTags6xHis, HA, and Vibrio cholerae MARTX toxin cystei…ExpressionBacterialMutationPromoterT7Available sinceJuly 19, 2012AvailabilityAcademic Institutions and Nonprofits only -
pET28a-HsSENP5
Plasmid#71468PurposeEncodes human full length SENP5 optimised for E. coli expression. Suitable for in vitro transcription/translation (T7).DepositorInsertSUMO1/sentrin specific peptidase 5 (SENP5 Human)
UseTagsHexahistidineExpressionBacterialMutationGene optimised for E. coli expression (Genscript)PromoterT7Available sinceDec. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET21a - CPX
Plasmid#89620Purpose[Edit] Expresses recombinant 6HIS tagged T. cruzi cytoplasmic tryparedoxin peroxidase protein in E. coli upon IPTG inductionDepositorInsertCytosolic Tryparedoxin peroxidase
UseTags6HIS and T7 tag (gene 10 leader)ExpressionBacterialMutationPromoterAvailable sinceMay 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET28b-Sad1
Plasmid#90297Purposeprotein expressionDepositorInsertSAD1
UseTagsHis tagExpressionBacterialMutationPromoterT7Available sinceAug. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET23a-MmRPS27a
Plasmid#69559PurposeBacterial expression; in vitro transcription/translation (T7)DepositorInsertRPS27A (RPS27A Human, Mouse)
UseTagsExpressionBacterialMutationChanged Alanine 133 (human RPS27a) to Glycine (mo…PromoterT7Available sinceDec. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET28a-HsSENP7
Plasmid#71470PurposeEncodes human full length SENP7. Suitable for in vitro transcription/translation (T7).DepositorInsertSUMO1/sentrin specific peptidase 7 (SENP7 Human)
UseTagsHexahistidineExpressionBacterialMutationGene optimised for E. coli expression (Genscript)PromoterT7Available sinceJan. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET28a Gx-NL2
Plasmid#169107PurposeExpresses protein G fused to 2 copies of NanoLuc. The protein G domain contains an amber stop-codon at position 24 for incorporation of the unnatural amino acid para-benzoylphenylalanine.DepositorInsertprotein G - NanoLuc - NanoLuc
UseTagshis-tag and strep-tagExpressionBacterialMutationAla 24 stopPromoterT7Available sinceJune 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET21b-RL018A
Plasmid#42137DepositorInsertmCerulean-mCherry
UseTagsExpressionBacterialMutationPromoterT7 promoterAvailable sinceJune 4, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET28a-BCA-P(11)4
Plasmid#185780PurposeEncodes enzyme bovine carbonic anhydrase linked to P(11)4 self-assembling peptide via a GS-linker sequenceDepositorInsertBCA-P(11)4
UseTagsExpressionBacterialMutationPromoterT7Available sinceJuly 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28b-API5
Plasmid#157655PurposeExpresses API5 in E.coli cellDepositorInsertApoptosis inhibitor 5 (API5 Human)
UseTagsHis tagExpressionBacterialMutationPromoterAvailable sinceAug. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET21a(+)-HpDsbG
Plasmid#39329DepositorInsertoxidoreductase
UseTagsHis-TagExpressionBacterialMutationPromoterT7Available sinceSept. 18, 2012AvailabilityAcademic Institutions and Nonprofits only -
pET26-CNTnw
Plasmid#100163Purposerecombinant expression of target protein as an MBP-His tag fusion at the N-terminus of target proteinDepositorInsertConcentrative nucleoside transporter
UseTagsMBP-HisExpressionBacterialMutationPromoterT7Available sinceOct. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET21a - SodB
Plasmid#89613PurposeExpresses recombinant 6HIS tagged T. cruzi superoxide dismutase B protein in E. coli upon IPTG inductionDepositorInsertSuperoxide dismutase B
UseTags6HIS and T7 tag (gene 10 leader)ExpressionBacterialMutationPromoterAvailable sinceApril 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET28a-T5-ASG_ClpXP
Plasmid#153299PurposeExpresses acoustic sensor genes or ClpXP-sensitive gas vesicles in E. coli Nissle 1917DepositorInsertASG
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterT5Available sinceSept. 21, 2020AvailabilityAcademic Institutions and Nonprofits only