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plko-tet-on-puromycin-shTRX1-259

Plasmid

#110920

Purpose

To knockdown Thioredoxin-1 following doxycycline addition

Depositor

Priyamvada Rai

Article

Samaranayake et al Nat Commun. 2017 Oct 31;8(1):1204. doi:

Insert

TRX1 (TXN Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

unknown

Available since

June 14, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_GTF2F1_TFIIF

Plasmid

#110037

Purpose

Protein expression and purification of GTF2F1_TFIIF

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

GTF2F1_TFIIF (GTF2F1 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pBABE.puro/TO/Flag/tGFP.HuR.S221D

Plasmid

#110351

Purpose

Doxycycline inducible mammalian retroviral expression of HuR (S221D) with FLAG and turboGFP tags

Depositor

Sandra Martha Gomes Dias

Insert

ELAV like RNA binding protein 1 (ELAVL1 Human)

Use

Retroviral

Tags

FLAG and tGFP

Expression

Mammalian

Mutation

S221D, P237S

Promoter

gag

Available since

Nov. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pBABE hygro MEN1 T344R

Plasmid

#11019

Depositor

Matthew Meyerson

Article

Hughes et al Mol Cell. 2004 Feb 27. 13(4):587-97.

Insert

MEN1 T344R (MEN1 Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

T344R

Promoter

Available since

Dec. 2, 2005

Availability

Academic Institutions and Nonprofits only
pQC V5 MCS IRES G418

Plasmid

#110345

Purpose

γ-Retroviral transfer vector for cloning and expressing your gene of interest. V5 N-Terminal tag, IRES-driven Geneticin selection.

Depositor

Sandra Martha Gomes Dias

Type

Empty backbone

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 22, 2018

Availability

Academic Institutions and Nonprofits only

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EGFP-PLEKHS1

Plasmid

#110509

Purpose

Fluorescent PLEKHS1

Depositor

Bernardo Mainou

Insert

PLEKHS1 (C10orf81 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

May 15, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3 HRPT2 L64P

Plasmid

#11052

Depositor

Matthew Meyerson

Article

Rozenblatt-Rosen et al Mol Cell Biol. 2005 Jan . 25(2):612-20.

Insert

HRPT2 L64P (CDC73 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

L64P

Promoter

Available since

Dec. 6, 2005

Availability

Academic Institutions and Nonprofits only
pRS316Nab3

Plasmid

#110533

Purpose

Expresses yeast NAB3 using the endogenous promoter

Depositor

Daniel Reines

Article

Loya et al Nucleic Acids Res. 2012 Aug;40(15):7476-

Insert

NAB3 (NAB3 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

June 19, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti-VQRRA-P2A-GFP-PGK-Puro

Plasmid

#110864

Purpose

Lentiviral vector for constitutive expression of Cas9-VQRRA-P2A-GFP in mammalian cells (codon optimized)

Depositor

Lukas Dow

Article

Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

Insert

Cas9-VQRRA

Use

Lentiviral

Tags

FLAG

Expression

Mutation

D1135V, R1335Q, T1337R and NLS sequence at the N-…

Promoter

EF1s

Available since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_APLP2_Kunitz-BPTI

Plasmid

#110041

Purpose

Protein expression and purification of APLP2_Kunitz-BPTI

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

APLP2_Kunitz-BPTI (APLP2 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pLenti-HF1-PGK-Puro

Plasmid

#110857

Purpose

Lentiviral vector for constitutive expression of Cas9-HF1 (not codon optimized)

Depositor

Lukas Dow

Article

Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

Insert

Cas9-HF1

Use

Lentiviral

Tags

3X FLAG

Expression

Mutation

N497A, R661A, Q695A, Q926A

Promoter

EF1s

Available since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_UBA52_Ubiquitin

Plasmid

#110043

Purpose

Protein expression and purification of UBA52_Ubiquitin

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

UBA52_Ubiquitin (UBA52 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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C. difficile 16S toehold switch sensor

Plasmid

#110704

Purpose

Toehold switch sensor to detect the V3 hypervariable region of the 16S rRNA with GFP output

Depositor

James Collins

Article

Takahashi et al Nat Commun. 2018 Aug 21;9(1):3347. doi:

Insert

C. difficile 16S toehold switch sensor

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

T7

Available since

Sept. 4, 2018

Availability

Academic Institutions and Nonprofits only

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pBABE hygro MEN1 W436R

Plasmid

#11018

Depositor

Matthew Meyerson

Article

Hughes et al Mol Cell. 2004 Feb 27. 13(4):587-97.

Insert

MEN1 W436R (MEN1 Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

W436R

Promoter

Available since

Dec. 2, 2005

Availability

Academic Institutions and Nonprofits only
Hy_pMT Flag-UAP56 MUT

Plasmid

#110124

Purpose

Expresses Flag-tagged human UAP56 (DDX39B) with 4 mutations that impact circRNA nuclear export activity

Depositor

Jeremy Wilusz

Article

Huang et al Genes Dev. 2018 May 17. pii: gad.314856.

Insert

UAP56 (DDX39B Human)

Use

Tags

Flag

Expression

Insect

Mutation

4 mutations (KSLN motif changed to RSFS)

Promoter

Metallothionein Promoter (pMT)

Available since

Sept. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3 HRPT2 227X

Plasmid

#11050

Depositor

Matthew Meyerson

Article

Rozenblatt-Rosen et al Mol Cell Biol. 2005 Jan . 25(2):612-20.

Insert

HRPT2 227X (CDC73 Human)

Use

Tags

flag

Expression

Mammalian

Mutation

truncation mutant with a stop codon introduced at…

Promoter

Available since

Dec. 6, 2005

Availability

Academic Institutions and Nonprofits only
pET27b-ecDHFR-N23PP/S148A

Plasmid

#110829

Purpose

Bacterial expression of E.coli DHFR N23PP/S148A mutant

Depositor

Stephen Benkovic

Article

Bhabha et al Science. 2011 Apr 8;332(6026):234-8. doi

Insert

ecDHFR

Use

Tags

Expression

Bacterial

Mutation

N23 mutated to P23; a proline residue inserted be…

Promoter

T7

Available since

June 6, 2018

Availability

Academic Institutions and Nonprofits only

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pBABE.puro/TO/Flag/mKO2

Plasmid

#110353

Purpose

Doxycycline inducible mammalian retroviral expression of mKO2 with FLAG tag

Depositor

Sandra Martha Gomes Dias

Insert

monomeric Kusabira Orange 2

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

gag

Available since

Nov. 6, 2018

Availability

Academic Institutions and Nonprofits only

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ISG15 C-term. Domain deltaC intein/chitin binding domain (79-154)

Plasmid

#110761

Purpose

Bacterial expression of proISG15

Depositor

David Komander

Article

Swatek et al Proc Natl Acad Sci U S A. 2018 Mar 6;115

Insert

ISG15 (ISG15 Human)

Use

Tags

intein/chitin binding domain

Expression

Bacterial

Mutation

Promoter

T7

Available since

June 13, 2018

Availability

Academic Institutions and Nonprofits only

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p180 pCIG-HDAC1 mut

Plasmid

#11053

Depositor

Ramesh Shivdasani

Article

Tou et al Mol Cell Biol. 2004 Apr . 24(8):3132-9.

Insert

HDAC1 (Hdac1 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Deletion of aa 1-56: deacetylase domain deletion

Promoter

Available since

Dec. 6, 2005

Availability

Academic Institutions and Nonprofits only