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  3. GPER-DuET

    Plasmid
    #213256
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    GPER (GPER1 Human)
    Use
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, T7
    Available since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213256-plasmid-map-sequence-id-430806

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  5. pCHAP3_Sap

    Plasmid
    #206849
    Purpose
    A plasmid containing a 17,271 bp fragment of the P. tricornutum chloroplast genome. The yeast marker URA3 was domesticated for SapI and integrated into the chloroplast fragment.
    Depositor
    Bogumil Karas
    Article
    Walker et al Plant Physiol. 2023 Dec 19:kiad670. doi:
    Type
    Empty backbone
    Use
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    Promoter
    Available since
    Jan. 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    206849-plasmid-map-sequence-id-419973

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  6. pCHAP2

    Plasmid
    #206847
    Purpose
    A plasmid containing a 15,249 bp fragment of the P. tricornutum chloroplast genome. The fragment is flanked by SapI recognition sites, allowing it to be released from the cloning vector pSAP1.
    Depositor
    Bogumil Karas
    Article
    Walker et al Plant Physiol. 2023 Dec 19:kiad670. doi:
    Type
    Empty backbone
    Use
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    Promoter
    Available since
    Jan. 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    206847-plasmid-map-sequence-id-419939

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  7. pCHAP1

    Plasmid
    #206846
    Purpose
    A plasmid containing a 14,519 bp fragment of the P. tricornutum chloroplast genome. The fragment is flanked by SapI recognition sites, allowing it to be released from the cloning vector pSAP1.
    Depositor
    Bogumil Karas
    Article
    Walker et al Plant Physiol. 2023 Dec 19:kiad670. doi:
    Type
    Empty backbone
    Use
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    Promoter
    Available since
    Jan. 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    206846-plasmid-map-sequence-id-419938

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  10. TFORF3334

    Plasmid
    #144810
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    SP100 (SP100 Human)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Aug. 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    144810-plasmid-map-sequence-id-404394

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  12. pPANI2A2E2-Crimson

    Plasmid
    #193758
    Purpose
    Expresses E2-Crimson under the control of PANI2 synthetic promoter, ColE1 origin of replication, Ampicillin selection
    Depositor
    Sangram Bagh
    Article
    Sarkar et al Chem Sci. 2021 Nov 9;12(48):15821-15832.
    Insert
    E2-Crimson
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PANI2 synthetic promoter carrying binding sites f…
    Available since
    April 7, 2023
    Availability
    Academic Institutions and Nonprofits only
    193758-plasmid-map-sequence-id-389741

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  14. pD649-HAsp-PDCD1LG2-Fc(DAPA)-AviTag-6xHis

    Plasmid
    #156612
    Purpose
    Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.
    Depositor
    Chris Garcia
    Article
    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.
    Insert
    PDCD1LG2 (PDCD1LG2 Human)
    Use
    Tags
    Fc(DAPA)-AviTag-6xHis
    Expression
    Mammalian
    Mutation
    Fc region of human IgG contains D265A and P329A m…
    Promoter
    CMV/SP6
    Available since
    March 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    156612-plasmid-map-sequence-id-302333

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  16. pDUAL_tacI

    Plasmid
    #155170
    Purpose
    BaSP and CuCBP co-expression: tacI promoter controls BaSP expression
    Depositor
    Bernd Nidetzky
    Article
    Schwaiger et al Biotechnol J. 2020 Jul 15:e2000063. doi:
    Inserts
    Sucrose Phosphorylase from Bifidobacterium adolescentis (BAD_RS00415 Bifidobacterium adolescentis)
    Cellobiose phosphorylase from Cellulomonas uda
    Use
    Synthetic Biology
    Tags
    His Tag and Strep - Tag II
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Sept. 22, 2020
    Availability
    Academic Institutions and Nonprofits only
    155170-plasmid-map-sequence-id-311666

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  25. pLV-mScarlet-I

    Plasmid
    #159172
    Purpose
    To express a bright monomeric red FP for labelling and detection of mammalian cells
    Depositor
    Pantelis Tsoulfas
    Insert
    mScarlet-I
    Use
    Lentiviral and Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Codons were optimized to Human codon usage
    Promoter
    CMV
    Available since
    Sept. 16, 2020
    Availability
    Academic Institutions and Nonprofits only
    159172-plasmid-map-sequence-id-311154

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  26. pAAV-hSyn-GRAB_ACh3.0 (AAV9)

    Viral Prep
    #121922-AAV9
    Purpose
    Ready-to-use AAV9 particles produced from pAAV-hSyn-GRAB_ACh3.0 (#121922). In addition to the viral particles, you will also receive purified pAAV-hSyn-GRAB_ACh3.0 plasmid DNA. Syn-driven expression of the genetically-encoded fluorescent acethycholine(ACh) sensor GRAB-ACh3.0. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Yulong Li
    Article
    Jing et al Nat Methods. 2020 Sep 28. pii: 10.1038/s
    Promoter
    Syn
    Tags
    None
    Available since
    June 21, 2024
    Availability
    Academic Institutions and Nonprofits only

  27. pAAV-EF1a-double floxed-hChR2(H134R)-EYFP-WPRE-HGHpA (AAV9)

    Viral Prep
    #20298-AAV9
    Purpose
    Ready-to-use AAV9 particles produced from pAAV-EF1a-double floxed-hChR2(H134R)-EYFP-WPRE-HGHpA (#20298). In addition to the viral particles, you will also receive purified pAAV-EF1a-double floxed-hChR2(H134R)-EYFP-WPRE-HGHpA plasmid DNA. EF1a-driven, Cre-dependent, humanized channelrhodopsin H134R mutant fused to EYFP, for optogenetic activation. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Karl Deisseroth
    Promoter
    EF1a
    Tags
    EYFP (Cre-dependent)
    Available since
    March 30, 2018
    Availability
    Academic Institutions and Nonprofits only

  28. pENN.AAV.hSyn.Cre.WPRE.hGH (AAV1)

    Viral Prep
    #105553-AAV1
    Purpose
    Ready-to-use AAV1 particles produced from pENN.AAV.hSyn.Cre.WPRE.hGH (#105553). In addition to the viral particles, you will also receive purified pENN.AAV.hSyn.Cre.WPRE.hGH plasmid DNA. hSyn-driven Cre expression. These AAV preparations are suitable purity for injection into animals.
    Depositor
    James M. Wilson
    Promoter
    Syn
    Tags
    None
    Available since
    May 15, 2018
    Availability
    Academic Institutions and Nonprofits only

  29. pAAV-hSyn-DIO-hM3D(Gq)-mCherry (AAV5)

    Viral Prep
    #44361-AAV5
    Purpose
    Ready-to-use AAV5 particles produced from pAAV-hSyn-DIO-hM3D(Gq)-mCherry (#44361). In addition to the viral particles, you will also receive purified pAAV-hSyn-DIO-hM3D(Gq)-mCherry plasmid DNA. Syn-driven, Cre-dependent, hM3D(Gq) receptor with an mCherry reporter for CNO-induced neuronal activation. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Bryan Roth
    Article
    Krashes et al J Clin Invest. 2011 Apr;121(4):1424-8. d
    Promoter
    Syn
    Tags
    mCherry (Cre-dependent)
    Available since
    Dec. 1, 2016
    Availability
    Academic Institutions and Nonprofits only
Showing: 721 - 750 of 158784 results