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Plasmid#228906PurposeExpression of mtPyronicSF in neurons targeted to the mitochondria serving as an optical sensor for mitochondrial pyruvate uptake. Backbone from Addgene plasmid 100834DepositorInsertpyronic SF
UseAAVTagsCox8 targettingExpressionMammalianMutationPromoterCamKIIAvailable sinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pX552-hsyn DIO GCaMP8f(with gRNA scaffold)
Plasmid#199580PurposeExpresses GCaMP8f in a Cre-dependent mannerDepositorInsertHis-DIO GCaMP8f
UseAAV, CRISPR, and Cre/LoxTags6xHisExpressionMammalianMutationPromoterhsynAvailable sinceOct. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pX552-EF1a-DIO Chronos-eGFP(with gRNA scaffold)
Plasmid#199582PurposeExpresses Chronos-eGFP in a Cre-dependent mannerDepositorInsertChronos eGFP
UseAAV, CRISPR, and Cre/LoxTagsGFPExpressionMammalianMutationPromoterEF1a intron AAvailable sinceOct. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
RNF130 shRNA
Plasmid#225340PurposeshRNAmir backbone for RNA interference, with YFPDepositorInsertRNF130 shRNA (Rnf130 Mouse)
UseAAV and RNAiTagsExpressionMammalianMutationPromoterCBAAvailable sinceSept. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
USP7 shRNA-TRE
Plasmid#225338PurposeshRNAmir backbone under tet-operator for RNA interference, with YFPDepositorInsertUSP7 shRNA (Usp7 Mouse)
UseAAV and RNAiTagsExpressionMammalianMutationPromoterTRE (tetracycline-responsive element)Available sinceSept. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEF1α:flex-mcherry
Plasmid#222618PurposeCre-dependent expression of the red fluorescent protein mCherry under control of the EF1a promoterDepositorInsertCre-dependent mCherry
UseAAVTagsExpressionMutationPromoterAvailable sinceJuly 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRDA_889
Plasmid#216080PurposeITRs from AAV2, destination vectorDepositorHas ServiceCloning Grade DNAInsertITRs from AAV2
UseAAV; DestinationTagsExpressionMammalianMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
U6-sgRNAs-Crym
Plasmid#200067PurposeFor the knock down of Crym with an astrocyte specific mCherry expressionDepositorInsertU6-3xsgRNA-Crym
UseAAV, CRISPR, and Mouse TargetingTagsGfaABC1D-mCherryExpressionMutationPromoterU6, GfaABC1DAvailable sinceApril 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
CAG-mCherry-sesRNA-GFP-WPRE-pA
Plasmid#192065PurposeExpression of mCherry, sesRNA in mammalian cells, with GFP as efRNA.DepositorInsertsesRNA for Cheta
UseAAVTagsExpressionMammalianMutationPromoterCAGAvailable sinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
IL1RN gRNA4
Plasmid#113133PurposeExpresses gRNA targeting the IL1RN promoter (SpyCas9 scaffold)DepositorInsertIL1RN guideRNA 4 (SpCas9 scaffold), co-expressed GFP (transfection marker)
UseAAV, Synthetic Biology, and TALENTagsExpressionMammalianMutationPromoterAvailable sinceNov. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
IL1RN gRNA2
Plasmid#113131PurposeExpresses gRNA targeting the IL1RN promoter (SpyCas9 scaffold)DepositorInsertIL1RN guideRNA 1 (SpCas9 scaffold), co-expressed GFP (transfection marker)
UseAAV, CRISPR, and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceNov. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pENN239_CRISPRcharm_Kv1
Plasmid#220838PurposeCRISPRcharm Kv1 expression in mammalian cellsDepositorInsertCRISPRcharm Kv1
UseCRISPRTagsP2A-TagBFPExpressionMammalianMutationN/APromoterCAGAvailable sinceJune 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pENN236_CRISPRcharm_Kv2
Plasmid#220839PurposeCRISPRcharm Kv2 expression in mammalian cellsDepositorInsertCRISPRcharm Kv2
UseCRISPRTagsP2A-TagBFPExpressionMammalianMutationN/APromoterCAGAvailable sinceJune 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
Cas12j-8-puro-crRNA
Plasmid#194965PurposeThe plasmid expresses human codon-optimized Cas12j-8, crRNA expression elements and puromycin resistence gene.DepositorTypeEmpty backboneUseAAVTagsExpressionMammalianMutationPromoterAvailable sinceApril 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
Cas12j-8-puro
Plasmid#194966PurposeThe plasmid expresses human codon-optimized Cas12j-8 and puromycin resistence gene.DepositorTypeEmpty backboneUseAAVTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEMS2115
Plasmid#49140PurposeThe plasmid contains the Pleaides (Ple) MiniPromoter Ple155 derived from the human PCP2 gene and designed for expression in ON Bipolar cells of the retina.DepositorInsertssAAV-Ple155-emGFP-WPRE
UseAAVTagsExpressionMammalianMutationPromoterMiniPromoter Ple155 from the human PCP2 geneAvailable sinceMay 10, 2016AvailabilityIndustry, Academic Institutions, and Nonprofits -
pENN226_CRISPRcharm
Plasmid#220834PurposeCRISPRcharm expression in mammalian cellsDepositorInsertCRISPRcharm
UseCRISPRTagsP2A-TagBFPExpressionMammalianMutationN/APromoterCAGAvailable sinceJune 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pENN496_miniCRISPRcharm_dSaCas9
Plasmid#220837Purposemini CRISPRcharm expression in mammalian cellsDepositorInsertmini CRISPRcharm
UseCRISPRTagsP2A-TagBFPExpressionMammalianMutationN/APromoterEFSAvailable sinceJune 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
GfaABC1D BARK D110A p2A mCherry
Plasmid#117692PurposeGfaABC1D-iBARK(D110A)-p2A-mCherry: Negative control viral expression vector for astrocyte Gaq silencingDepositorInsertBARKrgs D110A peptide
UseAAVTagsHA / FLAGExpressionMutationD110APromoterAvailable sinceMay 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
SlugCas9-HF
Plasmid#163796PurposeExpresses highly specific SlugCas9, and cloning backbone for sgRNADepositorTypeEmpty backboneUseAAV and CRISPRTagsExpressionMammalianMutationSlugCas9 (R247A, N415A, T421A, R656A)PromoterAvailable sinceMarch 18, 2021AvailabilityAcademic Institutions and Nonprofits only