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Plasmid#204516PurposeBacterial expression of COH-3 C-terminal fragment with 6xHis and maltose binding protein tags for use as an antigenDepositorInsertC-terminal fragment of coh-3 (C. elegans)
UseTags6xHis, MBPExpressionBacterialMutationPromoterT7lacAvailable sinceAug. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHIT186
Plasmid#204513PurposeBacterial expression of CSR-1 PAZ domain with 6xHis and maltose binding protein tags for use as an antigenDepositorInsertPAZ domain of csr-1 (C. elegans) (csr-1 Nematode)
UseTags6xHis, MBPExpressionBacterialMutationPromoterT7lacAvailable sinceAug. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHIT521
Plasmid#204520PurposeBacterial expression of CEC-5 N-terminal fragment with 6xHis and maltose binding protein tags for in vitro assaysDepositorInsertN-terminal fragment of cec-5 (C. elegans)
UseTags6xHis, MBPExpressionBacterialMutationPromoterT7lacAvailable sinceAug. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHIT182
Plasmid#204511PurposeBacterial expression of catalytically defective CSR-1a with 6xHis and maltose binding protein tags for in vitro assaysDepositorInsertcsr-1a with D769A mutation (C. elegans) (csr-1 Nematode)
UseTags6xHis, MBPExpressionBacterialMutationD769APromoterT7lacAvailable sinceAug. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHIT520
Plasmid#204519PurposeBacterial expression of CEC-8 N-terminal fragment with 6xHis and maltose binding protein tags for in vitro assaysDepositorInsertN-terminal fragment of cec-8 (C. elegans) (Y55B1BR.3 Nematode)
UseTags6xHis, MBPExpressionBacterialMutationPromoterT7lacAvailable sinceAug. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHIT342
Plasmid#204514PurposeBacterial expression of SYP-1 N-terminal fragment with 6xHis and maltose binding protein tags for use as an antigenDepositorInsertN-terminal fragment of syp-1 (C. elegans)
UseTags6xHis, MBPExpressionBacterialMutationPromoterT7lacAvailable sinceAug. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHIT320
Plasmid#204517PurposeBacterial expression of HIM-1 internal fragment with 6xHis and maltose binding protein tags for use as an antigenDepositorInsertInternal fragment of him-1 (C. elegans) (him-1 Nematode)
UseTags6xHis, MBPExpressionBacterialMutationPromoterT7lacAvailable sinceAug. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSLS.539
Plasmid#184958PurposeTest effect of a1/a2 length on editing fabH using retron recombineeringDepositorInsertEco1 RT and recombineering ncRNA, fabH G954A, a1/a2 length: 12
UseTagsExpressionBacterialMutationfabH donor G954APromoterT7/lacAvailable sinceJan. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
PRPF4.001.169
Plasmid#137233PurposeExpresses N-terminal (His)6-TEV cleavage site fusion of human gene or portion of gene in bacterial strains. pET based vector.DepositorInsertPRPF4 (PRPF4 Human)
UseTags(His)6-TEVExpressionBacterialMutation001-169PromoterAvailable sinceNov. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSLS.543
Plasmid#184960PurposeTest effect of a1/a2 length on editing murF using retron recombineeringDepositorInsertEco1 RT and recombineering ncRNA, murF G1359A, a1/a2 length: 12
UseTagsExpressionBacterialMutationmurF donor G1359APromoterT7/lacAvailable sinceNov. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSLS.545
Plasmid#184962PurposeTest effect of a1/a2 length on editing priB using retron recombineeringDepositorInsertEco1 RT and recombineering ncRNA, priB G1069A, a1/a2 length: 12
UseTagsExpressionBacterialMutationpriB donor G1069APromoterT7/lacAvailable sinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
ppwd1.473.646.W601A
Plasmid#137660PurposeExpresses N-terminal (His)6-Thrombin cleavage site fusion of human gene or portion of gene with point mutation in bacterial strains. pET based vector.DepositorInsertppwd1 (PPWD1 Human)
UseTags(His)6-thrombinExpressionBacterialMutation473-646-W601APromoterAvailable sinceAug. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pPD3cC
Plasmid#86972PurposeExpresses PCNA3 variant (R112C/T180C) fused phosphite dehydrogenase and P450cam in E. coliDepositorInsertPCNA3 variant (R112C/T180C) fused to phosphite dehydrogenase and P450cam
UseTagsHis6 tagExpressionBacterialMutationE175A/A176R in PTDH, N106R/R112C/T180C in PCNA3, …PromoterT7 promoterAvailable sinceMarch 15, 2017AvailabilityAcademic Institutions and Nonprofits only -
p1G108CP20X
Plasmid#85096PurposeExpresses PCNA1 variant (G108C) fused to putidaredoxin in E. coliDepositorInsertThe G108C variant of PCNA1 fused to putidaredoxin
UseTagsHis6 tagExpressionBacterialMutationG108C in PCNA1PromoterT7 promoterAvailable sinceNov. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGS-3R112C/T180C
Plasmid#85095PurposeHost vector to fuse a gene at the N-terminus of PCNA3 variant (R112C/T180C)DepositorInsertDNA polymerase sliding clamp 3
UseTagsHis6 tagExpressionBacterialMutationchanged Arginine 112 to Cysteine and Threonine 18…PromoterT7 promoterAvailable sinceNov. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
p1G108C-GG
Plasmid#85092PurposeHost vector to fuse a gene at the C-terminus of PCNA1 variant (G108C)DepositorInsertDNA polymerase sliding clamp 1
UseTagsHis6 tagExpressionBacterialMutationchanged Glycine 108 to CysteinePromoterT7 promoterAvailable sinceNov. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
Danio rerio cryaa V62T
Plasmid#47630PurposeRecombinant protein productionDepositorInsertAlpha A-crystallin (cryaa Zebrafish)
UseTagsExpressionBacterialMutationChanged Valine 62 to ThreoninePromoterT7Available sinceOct. 21, 2013AvailabilityAcademic Institutions and Nonprofits only -
Danio rerio cryaa T147V
Plasmid#47628PurposeRecombinant protein productionDepositorInsertAlpha A-crystallin (cryaa Zebrafish)
UseTagsExpressionBacterialMutationChanged Theronine 147 to ValinePromoterT7Available sinceSept. 30, 2013AvailabilityAcademic Institutions and Nonprofits only -
Danio rerio cryaa C143S
Plasmid#47619PurposeRecombinant protein productionDepositorInsertAlpha A-crystallin (cryaa Zebrafish)
UseTagsExpressionBacterialMutationCysteine 143 to SerinePromoterT7Available sinceSept. 30, 2013AvailabilityAcademic Institutions and Nonprofits only -
Lambda Phosphatase
Plasmid#79748PurposeIntended for co-expression with human Ser/Thr kinase plasmids to enhance bacterial kinase expression.DepositorInsertLambda Phosphatase
UseTagsExpressionBacterialMutationPromoterT7Available sinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only