Addgene

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piHGpd/G170D

Plasmid

#1328

Depositor

Susan Lindquist

Article

Nathan et al Proc Natl Acad Sci U S A 1997 Nov 25;94(

Insert

GPD promoter-hsp82 ts mutant (G170D) (HSP82 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

G170D

Promoter

Available since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only
R620-M15-303: CMV51p> SARS-CoV-2 S(1-1213)-2P-T4f-His6 D614G

Plasmid

#170201

Purpose

Mammalian expression of SARS-CoV-2 soluble spike trimer protein (D614G mutant)

Depositor

Dominic Esposito

Article

Esposito et al Protein Expr Purif. 2020 Jun 4;174:10568

Insert

SARS-CoV-2 S (spike) D614G (S SARS-CoV-2)

Use

Tags

His6

Expression

Mammalian

Mutation

D614G

Promoter

CMV51

Available since

Feb. 19, 2022

Availability

Academic Institutions and Nonprofits only

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R620-M07-303: CMV51p> TPAss-SARS-CoV-2 S-RBD(319-541)-His6 E484K

Plasmid

#170193

Purpose

Mammalian expression of SARS-CoV-2 spike receptor-binding domain (RBD), E484K mutant

Depositor

Dominic Esposito

Article

Mehalko et al Protein Expr Purif. 2021 Mar;179:105802.

Insert

SARS-CoV-2 RBD (S SARS-CoV-2)

Use

Tags

His6

Expression

Mammalian

Mutation

E484K

Promoter

CMV51

Available since

May 11, 2022

Availability

Academic Institutions and Nonprofits only

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R620-M03-303: CMV51p> TPAss-SARS-CoV-2 S-RBD(319-541)-His6 N501Y

Plasmid

#170189

Purpose

Mammalian expression of SARS-CoV-2 spike receptor-binding domain (RBD), N501Y mutant from B.1.1.7 variant

Depositor

Dominic Esposito

Article

Mehalko et al Protein Expr Purif. 2021 Mar;179:105802.

Insert

SARS-CoV-2 RBD (S SARS-CoV-2)

Use

Tags

His6

Expression

Mammalian

Mutation

N501Y

Promoter

CMV51

Available since

May 11, 2022

Availability

Academic Institutions and Nonprofits only

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pLENTI_hACE2_HygR

Plasmid

#155296

Purpose

Lentiviral vector to generate hACE2 stable expressing cell line, Hygromycin resistance

Depositor

Raffaele De Francesco

Article

Notarbartolo et al Sci Immunol. 2021 Aug 10;6(62). pii: 6/6

Insert

hACE2 (ACE2 Human)

Use

Lentiviral

Tags

C9

Expression

Mutation

Promoter

CMV

Available since

Aug. 5, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pCW-eGFP-FHA-SHLD2-m1

Plasmid

#114122

Purpose

Lentiviral vector for inducible expression of N-terminally tagged eGFP-RNF8 FHA domain-SHLD2, m1 mutant; 53BP1-independent recruitment to damaged chromatin

Depositor

Daniel Durocher

Article

Noordermeer et al Nature. 2018 Aug;560(7716):117-121. doi:

Insert

SHLD2 (m1 mutant) (SHLD2 Human)

Use

Lentiviral; Doxycycline inducible

Tags

RNF8 FHA domain (aa 2-160) and eGFP

Expression

Mammalian

Mutation

W489A, F494A, W495A mutations introduced

Promoter

TRE promoter, Tet ON

Available since

Aug. 28, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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pDEST-FRT/TO-3xFLAG-REV7

Plasmid

#114127

Purpose

Expresses N-terminally tagged 3x FLAG-REV7 in mammalian cells

Depositor

Daniel Durocher

Article

Noordermeer et al Nature. 2018 Aug;560(7716):117-121. doi:

Insert

REV7 (MAD2L2 Human)

Use

Genomic integration, tetracycline inducible

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

CMV/TetO2

Available since

Aug. 16, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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delta DE OCT4-GFP-2A-PURO transgenic reporter

Plasmid

#52381

Purpose

BAC recombineering generated construct, used as a reporter for OCT4 expression

Depositor

Jacob Hanna

Article

Gafni et al Nature. 2013 Dec 12;504(7479):282-6. doi

Inserts

GFP-2A-puro
OCT4 (POU5F1 Human)

Use

Tags

Expression

Mammalian

Mutation

deleted -3194 - -1768 upstream from OCT4 ATG

Promoter

OCT4

Available since

June 6, 2014

Availability

Academic Institutions and Nonprofits only

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delta PE OCT4-GFP-2A-PURO transgenic reporter

Plasmid

#52380

Purpose

BAC recombineering generated construct, used as a reporter for OCT4 expression

Depositor

Jacob Hanna

Article

Gafni et al Nature. 2013 Dec 12;504(7479):282-6. doi

Inserts

GFP-2A-puro
OCT4 (POU5F1 Human)

Use

Tags

Expression

Mammalian

Mutation

deleted -1620 - -1011 upstream from OCT4 ATG

Promoter

Available since

June 6, 2014

Availability

Academic Institutions and Nonprofits only

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mouse deltaPE Oct4-GFP transgenic reporter

Plasmid

#52382

Purpose

BAC recombineering generated construct, used as a reporter for mouse Oct4 expression

Depositor

Jacob Hanna

Article

Gafni et al Nature. 2013 Dec 12;504(7479):282-6. doi

Inserts

GFP pA
Oct4 (Pou5f1 Mouse)
PGK-gb2-Neo

Use

Tags

Expression

Mammalian

Mutation

deleted -300 - -1300bp fragment upstream from Oct…

Promoter

Oct4 and PGK+gb2

Available since

Sept. 8, 2014

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1 hMOG-beta2-EGFP

Plasmid

#160979

Purpose

Expresses a human myelin oligodendrocyte glycoprotein isoform beta2 - EGFP fusion protein in mammalian cells

Depositor

Markus Reindl

Article

Schanda et al Neurol Neuroimmunol Neuroinflamm. 2021 J

Insert

Homo sapiens myelin oligodendrocyte glycoprotein (MOG), transcript variant beta2 (MOG Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pGGG-Sr43

Plasmid

#186974

Purpose

Sr43 is a wheat stem rust resistance gene transferred from tall wheat grass (Thinopyrum ponticum) into bread wheat (Triticum aestivum).

Depositor

Brande Wulff

Article

Yu et al Nat Genet. 2023 Jun;55(6):921-926. doi:

Insert

Sr43

Use

Tags

None

Expression

Bacterial and Plant

Mutation

None

Promoter

Natice

Available since

April 4, 2023

Availability

Academic Institutions and Nonprofits only

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pCAGGS-PPP1CB-miRFP703

Plasmid

#178524

Purpose

Expresses PP1c-miRFP703 in mammalian cells

Depositor

Kazuhiro Aoki

Article

Yamamoto et al Nat Commun. 2021 Dec 8;12(1):7145. doi:

Insert

PP1c-miRFP703 (PPP1CB Synthetic, Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Dec. 21, 2021

Availability

Academic Institutions and Nonprofits only

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LentiV_Cas9_Blast

Plasmid

#125592

Purpose

Lentiviral expression of spCas9 with blasticidin resistance gene

Depositor

Christopher Vakoc

Article

Tarumoto et al Blood. 2019 Nov 1. pii: 422697. doi: 10.

Insert

Cas9

Use

CRISPR and Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

EFS promoter

Available since

June 10, 2019

Availability

Academic Institutions and Nonprofits only

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pEGFP hsp70

Plasmid

#15215

Depositor

Lois Greene

Article

Zeng et al J Cell Sci. 2004 Oct 1. 117(Pt 21):4991-

Insert

hsp70 (HSPA1A Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

June 15, 2007

Availability

Academic Institutions and Nonprofits only

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pAAV-TRE-DIO-FLPo

Plasmid

#118027

Purpose

AAV vector for sparse and bright labeling in a cell-type specific manner

Depositor

Minmin Luo

Article

Lin et al Nat Methods. 2018 Nov 19. pii: 10.1038/s

Insert

TRE-DIO-FLPo

Use

AAV

Tags

Expression

Mutation

Promoter

TRE

Available since

Feb. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pET14b-PITPalpha

Plasmid

#58256

Purpose

bacterial expression of human PITP alpha

Depositor

Shamshad Cockcroft

Article

Hara et al J Biol Chem. 1997 Jun 6;272(23):14908-13

Insert

PITP alpha (PITPNA Human)

Use

Tags

HIS

Expression

Bacterial

Mutation

Promoter

T7

Available since

July 28, 2014

Availability

Academic Institutions and Nonprofits only
HP21-TPI-WT-no_introns

Plasmid

#131044

Purpose

Expresses TPI-Wildtype NMD reporter without introns and with a 21 nt hairpin in 5'UTR

Depositor

Marvin Tanenbaum

Article

Hoek et al Mol Cell. 2019 May 23. pii: S1097-2765(1

Insert

TPI-Wildtype-no introns (TPI1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Pcmv

Available since

Sept. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pGL3-U6-pegRNA_SRD5A3-EGFP

Plasmid

#159781

Purpose

S. pyogenes pegRNA for C to A mutation at the SRD5A3 site of human cells using prime editing

Depositor

Xiaolong Wang

Article

Liu et al Cell Discov. 2020 Apr 28;6:27. doi: 10.1

Insert

spacer of pegRNA targeting SRD5A3 gene (SRD5A3 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pGL3-U6-sgRNA_SRD5A3-mcherry

Plasmid

#159782

Purpose

S. pyogenes sgRNA collocated with pegRNA targeting human SRD5A3 gene

Depositor

Xiaolong Wang

Article

Liu et al Cell Discov. 2020 Apr 28;6:27. doi: 10.1

Insert

spacer of sgRNA targeting SRD5A3 gene (SRD5A3 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 20, 2020

Availability

Academic Institutions and Nonprofits only

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